miR‑205 targets runt‑related transcription factor 2 to inhibit human pancreatic cancer progression

Zhuang,Lu; Guo,Jia; Yao,Yao; Li,Zhaoshen

doi:10.3892/ol.2018.9689

miR‑205 targets runt‑related transcription factor 2 to inhibit human pancreatic cancer progression

Authors:
- Lu Zhuang
- Jia Guo
- Yao Yao
- Zhaoshen Li
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Affiliations: Department of Gastroenterology, Changhai Hospital, Second Military Medical University, Shanghai 200433, P.R. China, Department of Cancer Biology, Mayo Clinic Florida, Jacksonville, FL 32224, USA
Published online on: November 12, 2018 https://doi.org/10.3892/ol.2018.9689
Pages: 843-848
Copyright: © Zhuang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have demonstrated that microRNAs (miRs) serve important roles in the progression of human cancer types, including pancreatic cancer (PC), a highly lethal malignancy. In the past few decades, several miRs have been identified to be associated with the overall survival of patients with PC and have been demonstrated to be potential therapeutic targets. However, to the best of our knowledge, the association between miR‑205 expression and the progression of PC has rarely been investigated. In the current study, low miR‑205 expression was revealed in PC tumor tissues and indicated poor prognosis in patients with PC. In addition, miR‑205 overexpression reduced and miR‑205 depletion enhanced PC cell proliferation and migration in vitro. Using bioinformatics, a luciferase reporter assay and western blot analyses, the current study identified that runt‑related transcription factor 2 (RUNX2) was a target of miR‑205 in PC and overexpression of miR‑205 suppressed the expression of RUNX2. Notably, overexpression of RUNX2 partially reversed the inhibitory effect of miR‑205 on PC cell proliferation and migration in vitro. Therefore, the results of the present study revealed that miR‑205 functions as a tumor suppressor in PC by targeting RUNX2.

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