Open Access

Ginsenoside Rg3 suppresses the proliferation of prostate cancer cell line PC3 through ROS‑induced cell cycle arrest

  • Authors:
    • Yanfei Peng
    • Ran Zhang
    • Xu Yang
    • Zhaiyi Zhang
    • Ning Kang
    • Liying Bao
    • Yongmei Shen
    • Hao Yan
    • Fang Zheng
  • View Affiliations

  • Published online on: November 12, 2018     https://doi.org/10.3892/ol.2018.9691
  • Pages: 1139-1145
  • Copyright: © Peng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

To investigate the potential antitumor effects of ginsenoside Rg3 in prostate cancer cells, the androgen‑insensitive prostate cancer cell line PC3 was cultured and incubated with ginsenoside Rg3 in vitro. Cell number counts, cell proliferation assays and senescence‑associated β‑galactosidase (SA‑β‑gal) staining were performed to evaluate cell proliferation. The results demonstrated that ginsenoside Rg3 led to cell proliferation arrest; ginsenoside Rg3 decreased the number of cells and increased the positive SA‑β‑gal staining rate in PC3 cells. Cell cycle analysis by flow cytometry revealed that ginsenoside Rg3 interfered with the G1/S transition in PC3 cells. The mechanism involved in ginsenoside Rg3‑induced cell proliferation arrest was then further investigated. This indicated that the level of reactive oxygen species (ROS) in PC3 cells was upregulated by ginsenoside Rg3 treatment. Furthermore, pretreatment with N‑acetyl‑L‑cysteine, a scavenger of ROS, was able to reverse the effects on cell number and cell cycle arrest induced by ginsenoside Rg3 in PC3 cells. These results indicate that ginsenoside Rg3 exhibits anticancer effects on prostate cancer cells through ROS‑mediated arrest of the cell cycle.
View Figures
View References

Related Articles

Journal Cover

January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Peng Y, Zhang R, Yang X, Zhang Z, Kang N, Bao L, Shen Y, Yan H and Zheng F: Ginsenoside Rg3 suppresses the proliferation of prostate cancer cell line PC3 through ROS‑induced cell cycle arrest. Oncol Lett 17: 1139-1145, 2019
APA
Peng, Y., Zhang, R., Yang, X., Zhang, Z., Kang, N., Bao, L. ... Zheng, F. (2019). Ginsenoside Rg3 suppresses the proliferation of prostate cancer cell line PC3 through ROS‑induced cell cycle arrest. Oncology Letters, 17, 1139-1145. https://doi.org/10.3892/ol.2018.9691
MLA
Peng, Y., Zhang, R., Yang, X., Zhang, Z., Kang, N., Bao, L., Shen, Y., Yan, H., Zheng, F."Ginsenoside Rg3 suppresses the proliferation of prostate cancer cell line PC3 through ROS‑induced cell cycle arrest". Oncology Letters 17.1 (2019): 1139-1145.
Chicago
Peng, Y., Zhang, R., Yang, X., Zhang, Z., Kang, N., Bao, L., Shen, Y., Yan, H., Zheng, F."Ginsenoside Rg3 suppresses the proliferation of prostate cancer cell line PC3 through ROS‑induced cell cycle arrest". Oncology Letters 17, no. 1 (2019): 1139-1145. https://doi.org/10.3892/ol.2018.9691