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Identification of dysregulated microRNAs in canine malignant melanoma

  • Authors:
    • Norio Ushio
    • Md Mahfuzur Rahman
    • Tadashi Maemura
    • Yu‑Chang Lai
    • Tomoko Iwanaga
    • Hiroaki Kawaguchi
    • Noriaki Miyoshi
    • Yasuyuki Momoi
    • Naoki Miura
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Veterinary Science, United Graduate School of Veterinary Science, Yamaguchi University, Yamaguchi 753‑8511, Japan, Kagoshima University Veterinary Teaching Hospital, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890‑0065, Japan, Department of Hygiene and Health Promotion Medicine, Graduate School of Medicine and Dental Sciences, Kagoshima University, Korimoto, Kagoshima 890‑0065, Japan, Department of Veterinary Histopathology, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890‑0065, Japan, Department of Veterinary Diagnostic Imaging, Joint Faculty of Veterinary Medicine, Kagoshima University, Korimoto, Kagoshima 890‑0065, Japan
    Copyright: © Ushio et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1080-1088
    |
    Published online on: November 12, 2018
       https://doi.org/10.3892/ol.2018.9692
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Abstract

Inhibiting aberrantly upregulated microRNAs (miR/miRNAs) has emerged as a novel focus for therapeutic intervention in human melanoma. Thus, identifying upregulated miRNAs is essential for identifying additional melanoma‑associated therapeutic targets. In the present study, microarray‑based miRNA profiling of canine malignant melanoma (CMM) tissue obtained from the oral cavity was performed and differential expression was confirmed by a reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). An analysis of the microarray data revealed 17 dysregulated miRNAs; 5 were upregulated and 12 were downregulated. RT‑qPCR analysis was performed for 2 upregulated (miR‑204 and miR‑383), 3 downregulated (miR‑122, miR‑143 and miR‑205) and 6 additional oncogenic miRNAs (oncomiRs; miR‑16, miR‑21, miR‑29b, miR‑92a, miR‑125b and miR‑222). The expression levels of seven of the miRNAs, miR‑16, miR‑21, miR‑29b, miR‑122, miR‑125b, miR‑204 and miR‑383 were significantly upregulated; however, the expression of miR‑205 was downregulated in CMM tissues compared with normal oral tissues. The microarray and RT‑qPCR analyses validated the upregulation of two potential oncomiRs miR‑204 and miR‑383. The present study additionally constructed a protein interaction network and a miRNA‑target regulatory interaction network using STRING and Cytoscape. In the proposed network, cyclin dependent kinase 2 was a target for miR‑383, sirtuin 1 and tumor protein p53 were targets for miR‑204 and ATR serine/threonine kinase was a target for both. It was concluded that miR‑383 and miR‑204 were potential oncomiRs that may be involved in regulating melanoma development by evading DNA repair and apoptosis.
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Spandidos Publications style
Ushio N, Rahman MM, Maemura T, Lai YC, Iwanaga T, Kawaguchi H, Miyoshi N, Momoi Y and Miura N: Identification of dysregulated microRNAs in canine malignant melanoma. Oncol Lett 17: 1080-1088, 2019.
APA
Ushio, N., Rahman, M.M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H. ... Miura, N. (2019). Identification of dysregulated microRNAs in canine malignant melanoma. Oncology Letters, 17, 1080-1088. https://doi.org/10.3892/ol.2018.9692
MLA
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17.1 (2019): 1080-1088.
Chicago
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17, no. 1 (2019): 1080-1088. https://doi.org/10.3892/ol.2018.9692
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Spandidos Publications style
Ushio N, Rahman MM, Maemura T, Lai YC, Iwanaga T, Kawaguchi H, Miyoshi N, Momoi Y and Miura N: Identification of dysregulated microRNAs in canine malignant melanoma. Oncol Lett 17: 1080-1088, 2019.
APA
Ushio, N., Rahman, M.M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H. ... Miura, N. (2019). Identification of dysregulated microRNAs in canine malignant melanoma. Oncology Letters, 17, 1080-1088. https://doi.org/10.3892/ol.2018.9692
MLA
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17.1 (2019): 1080-1088.
Chicago
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17, no. 1 (2019): 1080-1088. https://doi.org/10.3892/ol.2018.9692
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