Open Access

Identification of dysregulated microRNAs in canine malignant melanoma

  • Authors:
    • Norio Ushio
    • Md Mahfuzur Rahman
    • Tadashi Maemura
    • Yu‑Chang Lai
    • Tomoko Iwanaga
    • Hiroaki Kawaguchi
    • Noriaki Miyoshi
    • Yasuyuki Momoi
    • Naoki Miura
  • View Affiliations

  • Published online on: November 12, 2018     https://doi.org/10.3892/ol.2018.9692
  • Pages: 1080-1088
  • Copyright: © Ushio et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Inhibiting aberrantly upregulated microRNAs (miR/miRNAs) has emerged as a novel focus for therapeutic intervention in human melanoma. Thus, identifying upregulated miRNAs is essential for identifying additional melanoma‑associated therapeutic targets. In the present study, microarray‑based miRNA profiling of canine malignant melanoma (CMM) tissue obtained from the oral cavity was performed and differential expression was confirmed by a reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). An analysis of the microarray data revealed 17 dysregulated miRNAs; 5 were upregulated and 12 were downregulated. RT‑qPCR analysis was performed for 2 upregulated (miR‑204 and miR‑383), 3 downregulated (miR‑122, miR‑143 and miR‑205) and 6 additional oncogenic miRNAs (oncomiRs; miR‑16, miR‑21, miR‑29b, miR‑92a, miR‑125b and miR‑222). The expression levels of seven of the miRNAs, miR‑16, miR‑21, miR‑29b, miR‑122, miR‑125b, miR‑204 and miR‑383 were significantly upregulated; however, the expression of miR‑205 was downregulated in CMM tissues compared with normal oral tissues. The microarray and RT‑qPCR analyses validated the upregulation of two potential oncomiRs miR‑204 and miR‑383. The present study additionally constructed a protein interaction network and a miRNA‑target regulatory interaction network using STRING and Cytoscape. In the proposed network, cyclin dependent kinase 2 was a target for miR‑383, sirtuin 1 and tumor protein p53 were targets for miR‑204 and ATR serine/threonine kinase was a target for both. It was concluded that miR‑383 and miR‑204 were potential oncomiRs that may be involved in regulating melanoma development by evading DNA repair and apoptosis.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Ushio N, Rahman MM, Maemura T, Lai YC, Iwanaga T, Kawaguchi H, Miyoshi N, Momoi Y and Miura N: Identification of dysregulated microRNAs in canine malignant melanoma. Oncol Lett 17: 1080-1088, 2019
APA
Ushio, N., Rahman, M.M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H. ... Miura, N. (2019). Identification of dysregulated microRNAs in canine malignant melanoma. Oncology Letters, 17, 1080-1088. https://doi.org/10.3892/ol.2018.9692
MLA
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17.1 (2019): 1080-1088.
Chicago
Ushio, N., Rahman, M. M., Maemura, T., Lai, Y., Iwanaga, T., Kawaguchi, H., Miyoshi, N., Momoi, Y., Miura, N."Identification of dysregulated microRNAs in canine malignant melanoma". Oncology Letters 17, no. 1 (2019): 1080-1088. https://doi.org/10.3892/ol.2018.9692