Open Access

Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells

  • Authors:
    • Zhengfeng Xu
    • Jiajun Wu
    • Pan Cai
    • Xiaoxiao Zhou
    • Cunguo Yi
    • Bin Wang
  • View Affiliations

  • Published online on: November 14, 2018     https://doi.org/10.3892/ol.2018.9696
  • Pages: 877-882
  • Copyright: © Xu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Regulatory effects of fragile histidine triad (FHIT) gene on proliferation and apoptosis of osteosarcoma cells were studied. The hFOB1.19 and Saos2 cells were routinely cultured, pcDNA3.1‑FHIT overexpression vectors carrying FHIT gene fragments and blank pcDNA3.1 vectors were transfected into Saos2 cells, respectively, and the cells were divided into hFOB, Saos2, transfection and no‑load transfection groups. After transfection for 48 h, the cells were collected and analyzed. The expression of FHIT messenger ribonucleic acid (mRNA) was detected by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The expression of FHIT protein was detected by western blot analysis. Cell Counting Kit 8 (CCK8) was used to detect cell proliferation, and flow cytometry was used to detect apoptosis. The expression of FHIT mRNA was significantly decreased in Saos2 group compared with that in hFOB group, and the difference was statistically significant (P<0.05). The expression of FHIT mRNA was significantly increased in transfection group compared with that in Saos2 group, and the difference was statistically significant (P<0.05). The expression of FHIT protein was obviously decreased in Saos2 group compared with that in hFOB group, and there was a statistically significant difference (P<0.05). The expression of FHIT protein was obviously increased in transfection group compared with that in Saos2 group, and the difference was statistically significant (P<0.05). Compared with that in the hFOB group, the cell proliferation rate was remarkably increased in Saos2 group, while the apoptosis rate was remarkably decreased, showing statistically significant differences (P<0.05). Compared with those in Saos2 group, the cell proliferation rate was significantly decreased in transfection group, while the apoptosis rate was significantly increased, and the differences were statistically significant (P<0.05). In conclusion, FHIT gene regulates the proliferation and apoptosis of Saos2 osteosarcoma cells, inhibits the proliferation and promotes apoptosis of Saos2 osteosarcoma cells.
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January-2019
Volume 17 Issue 1

Print ISSN: 1792-1074
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Spandidos Publications style
Xu Z, Wu J, Cai P, Zhou X, Yi C and Wang B: Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells. Oncol Lett 17: 877-882, 2019.
APA
Xu, Z., Wu, J., Cai, P., Zhou, X., Yi, C., & Wang, B. (2019). Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells. Oncology Letters, 17, 877-882. https://doi.org/10.3892/ol.2018.9696
MLA
Xu, Z., Wu, J., Cai, P., Zhou, X., Yi, C., Wang, B."Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells". Oncology Letters 17.1 (2019): 877-882.
Chicago
Xu, Z., Wu, J., Cai, P., Zhou, X., Yi, C., Wang, B."Effects of FHIT gene on proliferation and apoptosis of osteosarcoma cells". Oncology Letters 17, no. 1 (2019): 877-882. https://doi.org/10.3892/ol.2018.9696