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Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells

  • Authors:
    • Pengfei Tao
    • Jing Feng
    • Qiong Li
    • Wei Liu
    • Lin Yang
    • Xiaolong Zhao
    • Huan Ni
    • Ping Xia
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, P.R. China, Department of Radiology, Taihe Hospital Hubei University of Medicine, Shiyan, Hubei 442000, P.R. China
    Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1791-1797
    |
    Published online on: November 20, 2018
       https://doi.org/10.3892/ol.2018.9739
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Abstract

The expression of micro-ribonucleic acid miR-664 and miR-184 on the biological characteristics of osteosarcoma (OS) SOSP-9607 cells was investigated. Eighteen surgical specimens of OS and 18 normal tissue specimens were collected. The expression of miR-664 and miR-184 was detected via fluorescence reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The OS cell line SOSP-9607 was selected as the object of study, and miR-664 blank group, miR-664 mimic group, miR-664 inhibitor group, miR-184 blank group, miR-184 mimic group and miR-184 inhibitor group were established through transfection. Changes in apoptosis were detected via flow cytometry, the cell proliferation capacity was detected via Cell Counting Kit-8 assay, and the cell migration was observed via wound healing assay. In cancer tissues of OS patients, the relative expression of miR-664 and miR-184 was significantly higher than that in para-carcinoma tissues (P<0.05). The cell growth in miR-664 inhibitor group was obviously decreased compared with those in miR-664 blank and mimic groups (P<0.05). There were differences in the cell migration capacity among groups (P<0.01), and the cell scratch areas in miR-664 and miR-184 mimic groups were significantly decreased compared with those in miR-664 and miR-184 blank groups (P<0.05), while they were significantly increased in miR-664 and miR-184 inhibitor groups compared with those in miR-664 and miR-184 blank and mimic groups (P<0.05, P<0.01). There were differences in the apoptosis rate among groups (P<0.01) and apoptosis in miR-664 and miR-184 inhibitor groups was remarkably increased compared with those in miR-664 and miR-184 blank and mimic groups (P<0.05). Downregulating the expression of miR-664 and miR-184 may promote apoptosis, inhibit the proliferation and reduce the migration capacity of SOSP-9607 cells. Therefore, miR-664 and miR-184 may provide a theoretical basis for the target selection in clinical targeted therapy and drug development for OS.
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Copy and paste a formatted citation
Spandidos Publications style
Tao P, Feng J, Li Q, Liu W, Yang L, Zhao X, Ni H and Xia P: Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells. Oncol Lett 17: 1791-1797, 2019.
APA
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X. ... Xia, P. (2019). Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells. Oncology Letters, 17, 1791-1797. https://doi.org/10.3892/ol.2018.9739
MLA
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X., Ni, H., Xia, P."Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 17.2 (2019): 1791-1797.
Chicago
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X., Ni, H., Xia, P."Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 17, no. 2 (2019): 1791-1797. https://doi.org/10.3892/ol.2018.9739
Copy and paste a formatted citation
x
Spandidos Publications style
Tao P, Feng J, Li Q, Liu W, Yang L, Zhao X, Ni H and Xia P: Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells. Oncol Lett 17: 1791-1797, 2019.
APA
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X. ... Xia, P. (2019). Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells. Oncology Letters, 17, 1791-1797. https://doi.org/10.3892/ol.2018.9739
MLA
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X., Ni, H., Xia, P."Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 17.2 (2019): 1791-1797.
Chicago
Tao, P., Feng, J., Li, Q., Liu, W., Yang, L., Zhao, X., Ni, H., Xia, P."Expression of miR-664 and miR-184 on proliferation, apoptosis and migration of osteosarcoma cells". Oncology Letters 17, no. 2 (2019): 1791-1797. https://doi.org/10.3892/ol.2018.9739
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