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Article

High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice

  • Authors:
    • Lijuan Xiu
    • Zhihui Yang
    • Ying Zhao
    • Xuan Liu
    • Jianpeng Jiao
    • Min Ye
    • Dazhi Sun
    • Pinkang Wei
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, Shanghai 200003, P.R. China, Department of Pathology, 81st Hospital Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210002, P.R. China
  • Pages: 1914-1920
    |
    Published online on: November 21, 2018
       https://doi.org/10.3892/ol.2018.9742
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Abstract

High‑fat diets (HFDs) are a risk factor for colorectal cancer. The present study investigated whether HFDs increase colon cancer metastasis in BALB/c mice. A total of 40 BALB/c mice were divided into four groups, including the tumor, tumor‑HFD, HFD and control groups. After 3 weeks, the tumor weights and metastases were observed. The serum levels of triglyceride, total cholesterol, lapin, interleukin‑6 (IL‑6) and tumor necrosis factor were analyzed using ELISA. The CD34, vascular endothelial growth factor (VEGF) and angiotensin 2 (ANG2) protein and mRNA levels in tumor tissues were analyzed with immunohistochemistry and reverse transcription‑polymerase chain reaction. The metastasis frequency increased in the tumor‑HFD group. However, there was no difference in the mean tumor weight between the tumor‑HFD and tumor groups. The serum cholesterol levels were increased in the tumor‑HFD and HFD groups compared with the control group. The levels of serum IL‑6 and tumor necrosis factor‑α were increased in the tumor‑HFD group compared with other groups. The CD34 protein level, and VEGF protein and mRNA levels were increased in the tumor‑HFD group compared with the tumor group. No difference was identified between the ANG2 protein and mRNA levels in of the two groups. It was concluded that HFD increased the serum level of cholesterol and cytokines, and potentially induced tumor angiogenesis, promoting transplanted orthotopic colon tumor metastasis in BALB/c mice.
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Copy and paste a formatted citation
Spandidos Publications style
Xiu L, Yang Z, Zhao Y, Liu X, Jiao J, Ye M, Sun D and Wei P: High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice. Oncol Lett 17: 1914-1920, 2019.
APA
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M. ... Wei, P. (2019). High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice. Oncology Letters, 17, 1914-1920. https://doi.org/10.3892/ol.2018.9742
MLA
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M., Sun, D., Wei, P."High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice". Oncology Letters 17.2 (2019): 1914-1920.
Chicago
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M., Sun, D., Wei, P."High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice". Oncology Letters 17, no. 2 (2019): 1914-1920. https://doi.org/10.3892/ol.2018.9742
Copy and paste a formatted citation
x
Spandidos Publications style
Xiu L, Yang Z, Zhao Y, Liu X, Jiao J, Ye M, Sun D and Wei P: High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice. Oncol Lett 17: 1914-1920, 2019.
APA
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M. ... Wei, P. (2019). High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice. Oncology Letters, 17, 1914-1920. https://doi.org/10.3892/ol.2018.9742
MLA
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M., Sun, D., Wei, P."High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice". Oncology Letters 17.2 (2019): 1914-1920.
Chicago
Xiu, L., Yang, Z., Zhao, Y., Liu, X., Jiao, J., Ye, M., Sun, D., Wei, P."High‑fat diets promote colon orthotopic transplantation tumor metastasis in BALB/c mice". Oncology Letters 17, no. 2 (2019): 1914-1920. https://doi.org/10.3892/ol.2018.9742
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