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Article Open Access

Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation

  • Authors:
    • Wen‑Ya Pan
    • Jiang‑Hui Zeng
    • Dong‑Yue Wen
    • Jie‑Yu Wang
    • Peng‑Peng Wang
    • Gang Chen
    • Zhen‑Bo Feng
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Ultrasonography, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China, Department of Nursing, First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi Zhuang Autonomous Region 530021, P.R. China
    Copyright: © Pan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1695-1713
    |
    Published online on: November 22, 2018
       https://doi.org/10.3892/ol.2018.9748
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Abstract

miR‑15b‑5p has frequently been reported to function as a biomarker in some malignancies; however, the function of miR‑15b‑5p in hepatocellular carcinoma (HCC) and its molecular mechanism are still not well understood. The present study was designed to confirm the clinical value of miR‑15b‑5p and further explore its underlying molecular mechanism. A comprehensive investigation of the clinical value of miR‑15b‑5p in HCC was investigated by data mining The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets as well as literature. In addition, intersected target genes of miR‑15b‑5p were predicted using the miRWalk database and differentially expressed genes of HCC from TCGA. Furthermore, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were carried out. Then, a protein‑protein interaction network (PPI) was constructed to reveal the interactions between some hub target genes of miR‑15b‑5p. The miR‑15b‑5p expression level in HCC was predominantly overexpressed compared with non‑HCC tissues samples (SMD=0.618, 95% CI: 0.207, 1.029; P<0.0001) based on 991 HCC and 456 adjacent non‑HCC tissue samples. The pooled summary receiver operator characteristic (SROC) of miR‑15b‑5p was 0.81 (Q*=0.74), and the pooled sensitivity and specificity of miR‑15b‑5p in HCC were 72% (95% CI: 69‑75%) and 68% (95% CI: 65‑72%), respectively. Bioinformatically, 225 overlapping genes were selected as prospective target genes of miR‑15b‑5p in HCC, and profoundly enriched GO terms and KEGG pathway investigation in silico demonstrated that the target genes were associated with prostate cancer, proximal tubule bicarbonate reclamation, heart trabecula formation, extracellular space, and interleukin‑1 receptor activity. Five genes (ACACB, RIPK4, MAP2K1, TLR4 and IGF1) were defined as hub genes from the PPI network. The high expression of miR‑15b‑5p could play an essential part in hepatocarcinogenesis through diverse regulation approaches.
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Copy and paste a formatted citation
Spandidos Publications style
Pan WY, Zeng JH, Wen DY, Wang JY, Wang PP, Chen G and Feng ZB: Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation. Oncol Lett 17: 1695-1713, 2019.
APA
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., & Feng, Z. (2019). Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation. Oncology Letters, 17, 1695-1713. https://doi.org/10.3892/ol.2018.9748
MLA
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., Feng, Z."Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation". Oncology Letters 17.2 (2019): 1695-1713.
Chicago
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., Feng, Z."Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation". Oncology Letters 17, no. 2 (2019): 1695-1713. https://doi.org/10.3892/ol.2018.9748
Copy and paste a formatted citation
x
Spandidos Publications style
Pan WY, Zeng JH, Wen DY, Wang JY, Wang PP, Chen G and Feng ZB: Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation. Oncol Lett 17: 1695-1713, 2019.
APA
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., & Feng, Z. (2019). Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation. Oncology Letters, 17, 1695-1713. https://doi.org/10.3892/ol.2018.9748
MLA
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., Feng, Z."Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation". Oncology Letters 17.2 (2019): 1695-1713.
Chicago
Pan, W., Zeng, J., Wen, D., Wang, J., Wang, P., Chen, G., Feng, Z."Oncogenic value of microRNA‑15b‑5p in hepatocellular carcinoma and a bioinformatics investigation". Oncology Letters 17, no. 2 (2019): 1695-1713. https://doi.org/10.3892/ol.2018.9748
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