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Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R

  • Authors:
    • Kai Zhang
    • Weiwei Wang
    • Yi Liu
    • Aijun Guo
    • Donghui Yang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Linyi Central Hospital, Linyi, Shandong 276400, P.R. China, Department of Obstetrics, Linyi Central Hospital, Linyi, Shandong 276400, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1646-1654
    |
    Published online on: December 4, 2018
       https://doi.org/10.3892/ol.2018.9793
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Abstract

MicroRNAs serve crucial functions in cancer progression by inhibiting the translation of target genes and causing mRNA degradation. However, the underlying regulatory mechanism of Let‑7b in osteosarcoma (OS) has not, to the best of our knowledge, been comprehensively elucidated. The aim of the present study was to investigate the function of Let‑7b in OS and clarify the regulation of insulin‑like growth factor 1 receptor (IGF1R) by Let‑7b. It was observed that Let‑7b was significantly downregulated in OS tissues and cell lines compared with the matched adjacent non‑tumorous tissues and human normal osteoblastic cell line hFOB 1.19. Overexpression of Let‑7b significantly inhibited the proliferation and invasion of U2OS and SAOS‑2 cells. A luciferase reporter assay validated that IGF1R was a downstream and functional target of Let‑7b. Let‑7b was also able to decrease the expression levels of IGF1R protein. Functional studies revealed that the antitumor effect of Let‑7b was probably due to targeting and suppressing IGF1R expression. Furthermore, in OS tissues, IGF1R was identified to be significantly upregulated and negatively correlated with Let‑7b levels. In conclusion, the results of the present study indicated that Let‑7b suppresses OS cellular proliferation and invasion via targeting IGF1R. A novel candidate prognostic factor was identified and it is suggested that the Let‑7b/IGF1R axis may represent a novel anti‑metastasis therapeutic target in OS.
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1 

Valery PC, Laversanne M and Bray F: Bone cancer incidence by morphological subtype: A global assessment. Cancer Causes Control. 26:1127–1139. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Mirabello L, Troisi RJ and Savage SA: Osteosarcoma incidence and survival rates from 1973 to 2004: Data from the surveillance, epidemiology, and end results program. Cancer. 115:1531–1543. 2009. View Article : Google Scholar : PubMed/NCBI

3 

Broadhead ML, Clark JC, Myers DE, Dass CR and Choong PF: The molecular pathogenesis of osteosarcoma: A review. Sarcoma. 2011:9592482011. View Article : Google Scholar : PubMed/NCBI

4 

Chou AJ, Geller DS and Gorlick R: Therapy for osteosarcoma: Where do we go from here? Paediatric Drugs. 10:315–327. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Friedman RC, Farh KK, Burge CB and Bartel DP: Most mammalian mRNAs are conserved targets of microRNAs. Genome Res. 19:92–105. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Kloosterman WP and Plasterk RH: The diverse functions of microRNAs in animal development and disease. Developmental Cell. 11:441–450. 2006. View Article : Google Scholar : PubMed/NCBI

7 

Croce CM and Calin GA: miRNAs, cancer, and stem cell division. Cell. 122:6–7. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Liang J, Liu C, Qiao A, Cui Y, Zhang H, Cui A, Zhang S, Yang Y, Xiao X, Chen Y, et al: MicroRNA-29a-c decrease fasting blood glucose levels by negatively regulating hepatic gluconeogenesis. J Hepatol. 58:535–542. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Chen L, Wang Q, Wang GD, Wang HS, Huang Y, Liu XM and Cai XH: miR-16 inhibits cell proliferation by targeting IGF1R and the Raf1-MEK1/2-ERK1/2 pathway in osteosarcoma. FEBS Lett. 587:1366–1372. 2013. View Article : Google Scholar : PubMed/NCBI

10 

Ji F, Zhang H, Wang Y, Li M, Xu W, Kang Y, Wang Z, Wang Z, Cheng P, Tong D, et al: MicroRNA-133a, downregulated in osteosarcoma, suppresses proliferation and promotes apoptosis by targeting Bcl-xL and Mcl-1. Bone. 56:220–226. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Kim HR, Shin CH, Lee H, Choi KH, Nam DH, Ohn T and Kim HH: MicroRNA-1908-5p contributes to the oncogenic function of the splicing factor SRSF3. Oncotarget. 8:8342–8355. 2017.PubMed/NCBI

12 

Reinhart BJ, Slack FJ, Basson M, Pasquinelli AE, Bettinger JC, Rougvie AE, Horvitz HR and Ruvkun G: The 21-nucleotide let-7 RNA regulates developmental timing in Caenorhabditis elegans. Nature. 403:901–906. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Pasquinelli AE, Reinhart BJ, Slack F, Martindale MQ, Kuroda MI, Maller B, Hayward DC, Ball EE, Degnan B, Müller P, et al: Conservation of the sequence and temporal expression of let-7 heterochronic regulatory RNA. Nature. 408:86–89. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Lan FF, Wang H, Chen YC, Chan C, Ng SS, Li K, Xie D, He ML, Lin MC and Kung HF: Hsa-let-7g inhibits proliferation of hepatocellular carcinoma cells by downregulation of c-Myc and upregulation of p16(INK4A). Int J Cancer. 128:319–331. 2011. View Article : Google Scholar : PubMed/NCBI

15 

Zhao C, Sun G, Li S, Lang MF, Yang S, Li W and Shi Y: MicroRNA let-7b regulates neural stem cell proliferation and differentiation by targeting nuclear receptor TLX signaling. Proc Nati Acad Sci USA. 107:1876–1881. 2010. View Article : Google Scholar

16 

Fukuma H, Beppu Y, Yokoyama R and Omiya K: TNM classification of bone and soft tissue sarcomas. Gan to kagaku Ryoho (Japanese). 24:1035–1040. 1997.

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Leichter AL, Sullivan MJ, Eccles MR and Chatterjee A: MicroRNA expression patterns and signalling pathways in the development and progression of childhood solid tumours. Mol Cancer. 16:152017. View Article : Google Scholar : PubMed/NCBI

19 

Tang N, Song WX, Luo J, Haydon RC and He TC: Osteosarcoma development and stem cell differentiation. Clin Orthop Relat Res. 466:2114–2130. 2008. View Article : Google Scholar : PubMed/NCBI

20 

Li S, Ferber A, Miura M and Baserga R: Mitogenicity and transforming activity of the insulin-like growth factor-I receptor with mutations in the tyrosine kinase domain. J Biol Chem. 269:32558–32564. 1994.PubMed/NCBI

21 

Kirschner LS: Inhibition of IGF-1R in adrenocortical carcinoma. Lancet Oncol. 16:356–357. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Maniscalco L, Iussich S, Morello E, Martano M, Gattino F, Miretti S, Biolatti B, Accornero P, Martignani E, Sánchez-Céspedes R, et al: Increased expression of insulin-like growth factor-1 receptor is correlated with worse survival in canine appendicular osteosarcoma. Vet J. 205:272–280. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Cao Y, Roth M, Piperdi S, Montoya K, Sowers R, Rao P, Geller D, Houghton P, Kolb EA, Gill J and Gorlick R: Insulin-like growth factor 1 receptor and response to anti-IGF1R antibody therapy in osteosarcoma. PLoS One. 9:e1062492014. View Article : Google Scholar : PubMed/NCBI

24 

Han K, Zhao T, Chen X, Bian N, Yang T, Ma Q, Cai C, Fan Q, Zhou Y and Ma B: MicroRNA-194 suppresses osteosarcoma cell proliferation and metastasis in vitro and in vivo by targeting CDH2 and IGF1R. Int J Oncol. 45:1437–1449. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Chen G, Fang T, Huang Z, Qi Y, Du S, Di T, Lei Z, Zhang X and Yan W: MicroRNA-133a inhibits osteosarcoma cells proliferation and invasion via targeting IGF-1R. Cell Physiol Biochem. 38:598–608. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang K, Wang W, Liu Y, Guo A and Yang D: Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R. Oncol Lett 17: 1646-1654, 2019.
APA
Zhang, K., Wang, W., Liu, Y., Guo, A., & Yang, D. (2019). Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R. Oncology Letters, 17, 1646-1654. https://doi.org/10.3892/ol.2018.9793
MLA
Zhang, K., Wang, W., Liu, Y., Guo, A., Yang, D."Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R". Oncology Letters 17.2 (2019): 1646-1654.
Chicago
Zhang, K., Wang, W., Liu, Y., Guo, A., Yang, D."Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R". Oncology Letters 17, no. 2 (2019): 1646-1654. https://doi.org/10.3892/ol.2018.9793
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang K, Wang W, Liu Y, Guo A and Yang D: Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R. Oncol Lett 17: 1646-1654, 2019.
APA
Zhang, K., Wang, W., Liu, Y., Guo, A., & Yang, D. (2019). Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R. Oncology Letters, 17, 1646-1654. https://doi.org/10.3892/ol.2018.9793
MLA
Zhang, K., Wang, W., Liu, Y., Guo, A., Yang, D."Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R". Oncology Letters 17.2 (2019): 1646-1654.
Chicago
Zhang, K., Wang, W., Liu, Y., Guo, A., Yang, D."Let‑7b acts as a tumor suppressor in osteosarcoma via targeting IGF1R". Oncology Letters 17, no. 2 (2019): 1646-1654. https://doi.org/10.3892/ol.2018.9793
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