Open Access

Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins

  • Authors:
    • Weihua Wang
    • Leilei Zhan
    • Dongqi Guo
    • Yanju Xiang
    • Yu Zhang
    • Muxing Tian
    • Zhanjiang Han
  • View Affiliations

  • Published online on: December 6, 2018     https://doi.org/10.3892/ol.2018.9807
  • Pages: 1741-1749
  • Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Grape seed proanthocyanidins (GSPs) have been demonstrated to exhibit potential chemotherapeutic efficacy against various cancer types. To determine the underlying molecular mechanisms involved in GSP‑induced apoptosis, the present study prepared pancreatic cancer (PC) cells samples, S3, S12 and S24, which were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control cell samples, C3, C12 and C24, were also prepared. Using RNA‑sequencing, transcriptome comparisons were performed, which identified 966, 3,543 and 4,944 differentially‑expressed genes (DEGs) in S3 vs. C3, S12 vs. C12 and S24 vs. C24, respectively. Gene Ontology analysis of the DEGs, revealed that treatment with GSPs is associated with disruption of the cell cycle (CC) in PC cells. Additionally, disruption of transcription, DNA replication and DNA repair were associated with GSP‑treatment in PC cells. Network analysis demonstrated that the common DEGs involved in the CC, transcription, DNA replication and DNA repair were integrated, and served essential roles in the control of CC progression in cancer cells. In summary, GSPs may exhibit a potential chemotherapeutic effect on PC cell proliferation.
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February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Wang W, Zhan L, Guo D, Xiang Y, Zhang Y, Tian M and Han Z: Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins. Oncol Lett 17: 1741-1749, 2019.
APA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Zhang, Y., Tian, M., & Han, Z. (2019). Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins. Oncology Letters, 17, 1741-1749. https://doi.org/10.3892/ol.2018.9807
MLA
Wang, W., Zhan, L., Guo, D., Xiang, Y., Zhang, Y., Tian, M., Han, Z."Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins". Oncology Letters 17.2 (2019): 1741-1749.
Chicago
Wang, W., Zhan, L., Guo, D., Xiang, Y., Zhang, Y., Tian, M., Han, Z."Transcriptome analysis of pancreatic cancer cell response to treatment with grape seed proanthocyanidins". Oncology Letters 17, no. 2 (2019): 1741-1749. https://doi.org/10.3892/ol.2018.9807