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Article

Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants

  • Authors:
    • Tokiko Nakai
    • Akihisa Sakurada
    • Toshihide Endo
    • Hiroko Kobayashi
    • Shinobu Masuda
    • Makoto Makishima
    • Mariko Esumi
  • View Affiliations / Copyright

    Affiliations: Division of Oncologic Pathology, Department of Pathology and Microbiology, Nara Medical University, Kashihara, Nara 634‑8521, Japan, Division of Biochemistry, Department of Biomedical Sciences, Nihon University School of Medicine, Tokyo 173‑8610, Japan
  • Pages: 1883-1888
    |
    Published online on: December 7, 2018
       https://doi.org/10.3892/ol.2018.9809
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Abstract

The mitochondrial DNA (mtDNA) displacement loop (D‑loop) is often altered in various cancer types, including with regard to simple sequence repeat number variation (SSRNV), which includes the C‑tract and CA‑tract. However, because of mitochondrial heteroplasmy and slippage errors by the Taq DNA polymerase used in polymerase chain reaction (PCR) analysis, it is difficult to precisely evaluate mtDNA D‑loop SSRNV experimentally. In this study, to precisely determine cancer‑specific variants in mtDNA SSRNV, various microscopic portions of cancerous tissues and normal control tissues were obtained from a patient with breast cancer, followed by laser‑capture microdissection of formalin‑fixed paraffin‑embedded specimens. Regions containing (CA)7 repeats (positions 514‑523) and (C)8 repeats (positions 303‑315) of the mitochondria DNA D‑loop were amplified and sequenced. Variant signals of mtDNA SSRs of (CA)7 and (C)8 were observed in normal and cancerous tissues, with the content of minor alleles (CA)6 and (C)7/(C)9 differing among samples. These results were confirmed by PCR using various primers and proofreading DNA polymerases. PCR of genomic SSRs of (CA)7 in the NAALD2 gene and (C)8 in the BMP6 gene showed a simple repeat in all samples that was different from the observed mtDNA SSRNV. The present study suggests a reliable procedure for determining cancer‑specific variants in mtDNA SSRNV: Using a proofreading DNA polymerase for PCR, the background of slippage by PCR is determined by PCR of the same genomic sequence as the target. Due to the varied heteroplasmy level of mtDNA SSRNV among normal tissues, the second background of polymorphic variations should be determined by several normal tissue DNA as PCR templates. Finally, the cancer‑specific variant, including its variation frequency, is determined by subtracting the two background signals from the variant signals in cancer. However, care must be taken, as normal heteroplasmy drifts observed in mtDNA SSRNV may complicate such estimations.
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Copy and paste a formatted citation
Spandidos Publications style
Nakai T, Sakurada A, Endo T, Kobayashi H, Masuda S, Makishima M and Esumi M: Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants. Oncol Lett 17: 1883-1888, 2019.
APA
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., & Esumi, M. (2019). Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants. Oncology Letters, 17, 1883-1888. https://doi.org/10.3892/ol.2018.9809
MLA
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., Esumi, M."Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants". Oncology Letters 17.2 (2019): 1883-1888.
Chicago
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., Esumi, M."Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants". Oncology Letters 17, no. 2 (2019): 1883-1888. https://doi.org/10.3892/ol.2018.9809
Copy and paste a formatted citation
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Spandidos Publications style
Nakai T, Sakurada A, Endo T, Kobayashi H, Masuda S, Makishima M and Esumi M: Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants. Oncol Lett 17: 1883-1888, 2019.
APA
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., & Esumi, M. (2019). Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants. Oncology Letters, 17, 1883-1888. https://doi.org/10.3892/ol.2018.9809
MLA
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., Esumi, M."Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants". Oncology Letters 17.2 (2019): 1883-1888.
Chicago
Nakai, T., Sakurada, A., Endo, T., Kobayashi, H., Masuda, S., Makishima, M., Esumi, M."Caution for simple sequence repeat number variation in the mitochondrial DNA D‑loop to determine cancer‑specific variants". Oncology Letters 17, no. 2 (2019): 1883-1888. https://doi.org/10.3892/ol.2018.9809
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