Open Access

Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion

  • Authors:
    • Shang‑Long Liu
    • Shou‑Gen Cao
    • Ying Li
    • Bo Sun
    • Dong Chen
    • Dong‑Sheng Wang
    • Yan‑Bing Zhou
  • View Affiliations

  • Published online on: December 10, 2018     https://doi.org/10.3892/ol.2018.9816
  • Pages: 2057-2062
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The biological features of pancreatic cancer and the associated hypoxic environment around the cancer cells often lead to resistance to radiotherapy and chemotherapy. The present study was performed in order to explore the effect pancreatic stellate cells (PSCs) have on the proliferation of pancreatic cancer cells. In the present study, PSCs from human pancreatic cancer tissues were isolated, and the PSCs markers α‑smooth muscle actin and desmin were overexpressed in the cytoplasm of PSCs. An MTT assay revealed that PSCs promoted the viability of pancreatic cancer cells. However, the viability of pancreatic cancer cells promoted by PSCs was partially blocked by SB525334. Cellular invasion analysis demonstrated that PSCs promoted the invasion ability of pancreatic cancer cells. An apoptosis assay indicated that PSCs decreased the level of apoptosis induced by gemcitabine. In vivo experiments consisting of mice bearing MIA‑PaCa‑2 and PSCs demonstrated an increase in the rate of tumor growth compared with MIA‑PaCA‑2 alone, whereas SB525334 may delay the tumor progression induced by PSCs. The present findings indicated that PSCs promoted the viability and invasion of pancreatic cancer cells, and decreased the apoptosis of pancreatic cancer cells induced by gemcitabine.
View Figures
View References

Related Articles

Journal Cover

February-2019
Volume 17 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Liu SL, Cao SG, Li Y, Sun B, Chen D, Wang DS and Zhou YB: Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion. Oncol Lett 17: 2057-2062, 2019
APA
Liu, S., Cao, S., Li, Y., Sun, B., Chen, D., Wang, D., & Zhou, Y. (2019). Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion. Oncology Letters, 17, 2057-2062. https://doi.org/10.3892/ol.2018.9816
MLA
Liu, S., Cao, S., Li, Y., Sun, B., Chen, D., Wang, D., Zhou, Y."Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion". Oncology Letters 17.2 (2019): 2057-2062.
Chicago
Liu, S., Cao, S., Li, Y., Sun, B., Chen, D., Wang, D., Zhou, Y."Pancreatic stellate cells facilitate pancreatic cancer cell viability and invasion". Oncology Letters 17, no. 2 (2019): 2057-2062. https://doi.org/10.3892/ol.2018.9816