miR‑7‑5p regulates the proliferation and migration of colorectal cancer cells by negatively regulating the expression of Krüppel‑like factor 4

Dong,Mingjun; Xie,Yangyang; Xu,Yidong

doi:10.3892/ol.2019.10001

miR‑7‑5p regulates the proliferation and migration of colorectal cancer cells by negatively regulating the expression of Krüppel‑like factor 4

Authors:
- Mingjun Dong
- Yangyang Xie
- Yidong Xu
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Affiliations: Department of Anorectal Surgery, The No. 2 Hospital of Ningbo, Ningbo, Zhejiang 315010, P.R. China
Published online on: February 1, 2019 https://doi.org/10.3892/ol.2019.10001
Pages: 3241-3246
Copyright: © Dong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Previous studies have demonstrated that microRNA‑7‑5p (miR‑7‑5p) functions as a tumor suppressor in certain types of human cancer. However, the role of miR‑7‑5p in colorectal cancer (CRC) remains to be investigated. Using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), the present study demonstrated that miR‑7‑5p was downregulated in CRC tissues and cell lines. In addition, low miR‑7‑5p expression is able to predict a poor 5‑year overall survival rate for patients with CRC. In vitro studies revealed that miR‑7‑5p overexpression inhibits cell proliferation and migration. Furthermore, Krüppel‑like factor 4 (KLF4), an oncogene in CRC, was validated as a direct target of miR‑7‑5p. KLF4 expression was negatively correlated with miR‑7‑5p expression in CRC tissues. Notably, KLF4 overexpression rescued the suppressive effects of miR‑7‑5p on CRC cell proliferation and migration. In summary, the results of this study demonstrated that miR‑7‑5p inhibits CRC proliferation and migration by targeting KLF4, which suggests that miR‑7‑5p is a potential molecular target for the treatment of human CRC.

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