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Article Open Access

Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells

  • Authors:
    • Yoshinori Inagaki
    • Tomohiko Tokunaga
    • Mitsuru Yanai
    • Dan Wu
    • Jiyi Huang
    • Hiroki Nagase
    • Noboru Fukuda
    • Toshinori Ozaki
    • Masayoshi Soma
    • Kyoko Fujiwara
  • View Affiliations / Copyright

    Affiliations: Division of General Medicine, Department of Medicine, Nihon University School of Medicine, Itabashi, Tokyo 173‑8610, Japan, Department of Oncology, The 5th Hospital of Xiamen, Xiamen, Fujian 361101, P.R. China, Department of Nephropathy, The 5th Hospital of Xiamen, Xiamen, Fujian 361101, P.R. China, Laboratory of Cancer Genetics, Chiba Cancer Center Research Institute, Chiba 260‑8717, Japan, Division of Nephrology, Hypertension and Endocrinology, Department of Medicine, Nihon University School of Medicine, Itabashi, Tokyo 173‑8610, Japan, Department of DNA Damage Signaling, Research Center, The 5th Hospital of Xiamen, Xiamen, Fujian 361101, P.R. China
    Copyright: © Inagaki et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3735-3742
    |
    Published online on: February 6, 2019
       https://doi.org/10.3892/ol.2019.10019
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Abstract

Erythropoietin‑producing hepatocellular (Eph) receptors and their ligand ephrins serve crucial roles in the interactions among epithelial cells. Eph receptor/ephrin signaling regulates cell functions, including proliferation, differentiation and migration, via these cell‑cell interactions. We reported previously that EPHB2, a member of the Eph receptor family, was highly expressed in chemically induced cutaneous squamous cell carcinoma (cSCC) tissues in mice. Although the higher expression level of EPHB2 has been observed in various human cancers, its roles in the development and progression of cancers are still unclear. In the present study, the functional implications of EPHB2 in the acquisition of malignant phenotypes of cSCC cells was investigated. Silencing of EPHB2 in the human cSCC cell line A431 induced epithelial‑mesenchymal transition (EMT)‑like morphological changes accompanied by a significant upregulation of epithelial‑mesenchymal transition‑associated genes such as zinc finger E‑box binding homeobox 1/2. In addition, silencing of EPHB2 suppressed anchorage‑independent cell growth under 3D culture conditions. Consistent with these observations, EPHB2 exhibited higher levels of expression in tumor spheres formed under 3D culture conditions than in cells cultured in adherent form, and the expression pattern of EMT markers indicated that EMT was suppressed in tumor spheres. The results of the present study indicated that EPHB2 serves a pivotal role in promoting the anchorage‑independent growth of A431 cells through the suppression of EMT.
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Copy and paste a formatted citation
Spandidos Publications style
Inagaki Y, Tokunaga T, Yanai M, Wu D, Huang J, Nagase H, Fukuda N, Ozaki T, Soma M, Fujiwara K, Fujiwara K, et al: Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells. Oncol Lett 17: 3735-3742, 2019.
APA
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H. ... Fujiwara, K. (2019). Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells. Oncology Letters, 17, 3735-3742. https://doi.org/10.3892/ol.2019.10019
MLA
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H., Fukuda, N., Ozaki, T., Soma, M., Fujiwara, K."Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells". Oncology Letters 17.4 (2019): 3735-3742.
Chicago
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H., Fukuda, N., Ozaki, T., Soma, M., Fujiwara, K."Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells". Oncology Letters 17, no. 4 (2019): 3735-3742. https://doi.org/10.3892/ol.2019.10019
Copy and paste a formatted citation
x
Spandidos Publications style
Inagaki Y, Tokunaga T, Yanai M, Wu D, Huang J, Nagase H, Fukuda N, Ozaki T, Soma M, Fujiwara K, Fujiwara K, et al: Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells. Oncol Lett 17: 3735-3742, 2019.
APA
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H. ... Fujiwara, K. (2019). Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells. Oncology Letters, 17, 3735-3742. https://doi.org/10.3892/ol.2019.10019
MLA
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H., Fukuda, N., Ozaki, T., Soma, M., Fujiwara, K."Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells". Oncology Letters 17.4 (2019): 3735-3742.
Chicago
Inagaki, Y., Tokunaga, T., Yanai, M., Wu, D., Huang, J., Nagase, H., Fukuda, N., Ozaki, T., Soma, M., Fujiwara, K."Silencing of EPHB2 promotes the epithelial‑mesenchymal transition of skin squamous cell carcinoma‑derived A431 cells". Oncology Letters 17, no. 4 (2019): 3735-3742. https://doi.org/10.3892/ol.2019.10019
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