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Article Open Access

The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling

  • Authors:
    • Jinlong Li
    • Zhaoli Zhang
    • Lixue Wang
    • Yongchen Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Laboratory Medicine, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, P.R. China, Department of Pharmacy, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, P.R. China, Department of Oncology, The Second Affiliated Hospital of Southeast University, Nanjing, Jiangsu 210003, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3657-3664
    |
    Published online on: February 13, 2019
       https://doi.org/10.3892/ol.2019.10035
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Abstract

Colorectal cancer (CRC) is one of the major causes of cancer‑associated mortality worldwide. Wnt family member 10A (Wnt10a) is an oncogene associated with the carcinogenesis and progression of renal cell carcinoma, and is strongly expressed in the CRC cell line SW480. However, the role of Wnt10a in CRC has been rarely reported. In the present study, the expression levels of Wnt10a were higher in 40 tumor tissues compared with in paired control tissues, as determined by RT‑qPCR method. In addition, the clinic opathological association analysis indicated that Wnt10a expression was associated with tumor stage (T3+T4, P=0.015). Furthermore, Wnt10a was highly expressed in the SW480, SW620 and HCT116 cell lines. In order to explore the role of Wnt10a in CRC, Wnt10a expression was knocked down by siRNA technology in HCT116 cell line. Cell proliferation was significantly inhibited by 55% in CCK‑8 assay following Wnt10a knockdown and cell migration rate was decreased by 50% in Transwell assay. In addition, western blot analysis demonstrated that Wnt10a knockdown decreased the expression levels of β‑catenin, cyclin D1, lymphoid enhancer‑binding factor 1 and protein kinase B, which was consistent with results obtained with the Wnt/β‑catenin specific inhibitor LGK‑974. It was thus suggested that Wnt10a downregulation inactivated the Wnt/β‑catenin signaling pathway in HCT116 cells. In conclusion, the present study demonstrated that Wnt10a may have an oncogenic role during carcinogenesis of CRC through activation of Wnt/β‑catenin signaling.
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Copy and paste a formatted citation
Spandidos Publications style
Li J, Zhang Z, Wang L and Zhang Y: The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling. Oncol Lett 17: 3657-3664, 2019.
APA
Li, J., Zhang, Z., Wang, L., & Zhang, Y. (2019). The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling. Oncology Letters, 17, 3657-3664. https://doi.org/10.3892/ol.2019.10035
MLA
Li, J., Zhang, Z., Wang, L., Zhang, Y."The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling". Oncology Letters 17.4 (2019): 3657-3664.
Chicago
Li, J., Zhang, Z., Wang, L., Zhang, Y."The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling". Oncology Letters 17, no. 4 (2019): 3657-3664. https://doi.org/10.3892/ol.2019.10035
Copy and paste a formatted citation
x
Spandidos Publications style
Li J, Zhang Z, Wang L and Zhang Y: The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling. Oncol Lett 17: 3657-3664, 2019.
APA
Li, J., Zhang, Z., Wang, L., & Zhang, Y. (2019). The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling. Oncology Letters, 17, 3657-3664. https://doi.org/10.3892/ol.2019.10035
MLA
Li, J., Zhang, Z., Wang, L., Zhang, Y."The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling". Oncology Letters 17.4 (2019): 3657-3664.
Chicago
Li, J., Zhang, Z., Wang, L., Zhang, Y."The oncogenic role of Wnt10a in colorectal cancer through activation of canonical Wnt/β‑catenin signaling". Oncology Letters 17, no. 4 (2019): 3657-3664. https://doi.org/10.3892/ol.2019.10035
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