L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines

  • Authors:
    • Ayumu Nagamine
    • Takuya Araki
    • Daisuke Nagano
    • Mitsue Miyazaki
    • Koujirou Yamamoto
  • View Affiliations

  • Published online on: February 26, 2019     https://doi.org/10.3892/ol.2019.10075
  • Pages: 4710-4716
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Abstract

Recently, proteins derived from cancer cells have been widely investigated as biomarkers for predicting the efficacy of chemotherapy. In this study, to identify a sensitive biomarker for the efficacy of anti‑epidermal growth factor receptor monoclonal antibodies (anti‑EGFR mAbs), proteins derived from 6 colorectal cancer (CRC) cell lines with different sensitivities to cetuximab, an anti‑EGFR mAb, were analyzed. Cytoplasmic and membrane proteins extracted from each CRC cell line were digested using trypsin and analyzed comprehensively using mass spectrometry. As a result, 148 and 146 peaks from cytoplasmic proteins and 363 and 267 peaks from membrane proteins were extracted as specific peaks for cetuximab‑resistant and ‑sensitive CRC cell lines, respectively. By analyzing the proteins identified from the peptide peaks, cytoplasmic L‑lactate dehydrogenase B (LDHB) was detected as a marker of cetuximab sensitivity, and it was confirmed that LDHB expression was increased in cetuximab‑resistant CRC cell lines. Furthermore, LDHB expression levels were significantly upregulated with the acquisition of resistance to cetuximab in cetuximab‑sensitive CRC cell lines. In conclusion, LDHB was identified as an important factor affecting cetuximab sensitivity using comprehensive proteome analysis for the first time.
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May-2019
Volume 17 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Nagamine A, Araki T, Nagano D, Miyazaki M and Yamamoto K: L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines. Oncol Lett 17: 4710-4716, 2019
APA
Nagamine, A., Araki, T., Nagano, D., Miyazaki, M., & Yamamoto, K. (2019). L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines. Oncology Letters, 17, 4710-4716. https://doi.org/10.3892/ol.2019.10075
MLA
Nagamine, A., Araki, T., Nagano, D., Miyazaki, M., Yamamoto, K."L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines". Oncology Letters 17.5 (2019): 4710-4716.
Chicago
Nagamine, A., Araki, T., Nagano, D., Miyazaki, M., Yamamoto, K."L‑Lactate dehydrogenase B may be a predictive marker for sensitivity to anti‑EGFR monoclonal antibodies in colorectal cancer cell lines". Oncology Letters 17, no. 5 (2019): 4710-4716. https://doi.org/10.3892/ol.2019.10075