Knockdown of ISOC1 suppresses cell proliferation in pancreatic cancer in vitro

Cheng,Li; Zhao,Yan; Tang,Maochun; Luo,Zhengsheng; Wang,Xingpeng

doi:10.3892/ol.2019.10082

May-2019 Volume 17 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-2019 Volume 17 Issue 5

Full Size Image

Article Open Access

Knockdown of ISOC1 suppresses cell proliferation in pancreatic cancer in vitro

Authors:
- Li Cheng
- Yan Zhao
- Maochun Tang
- Zhengsheng Luo
- Xingpeng Wang
View Affiliations / Copyright

Affiliations: Department of Gastroenterology, Shanghai General Hospital of Nanjing Medical University, Shanghai 200080, P.R. China, Department of Gastroenterology, School of Medicine, Shanghai Tenth People's Hospital, Tongji University, Shanghai 200072, P.R. China

Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 4263-4270
|
Published online on: February 28, 2019

https://doi.org/10.3892/ol.2019.10082
Expand metrics +

Abstract

Pancreatic cancer is a deadly disease that is frequently associated with mortality at the time of diagnosis due to rapid metastasis, which makes it unsuitable for operative surgery, and resistant to chemotherapy and radiation therapy. Isochorismatase domain‑containing protein 1 (ISOC1) has putative isochorismatase activity, and is positively regulated by estrogen in human breast cancer. However, its role in pancreatic cancer has yet to be fully elucidated. Analysis from datasets downloaded from The Cancer Genome Atlas and Genotype‑Tissue Expression databases indicated that the ISOC1 mRNA expression level was increased in pancreatic cancer tissues, compared with normal pancreatic tissues. In the present study, it was determined that the human pancreatic cancer cell lines SW 1990, PANC‑1 and AsPC‑1 had increased expression levels of ISOC1 mRNA, compared with human pancreatic ductal epithelial cells. Additionally, two of the pancreatic cancer cell lines, SW 1990 and PANC‑1, transfected with lentivirus‑delivered short hairpin RNA, to knockdown the expression of ISOC1, were established. Cell counting and MTT assays indicated that knockdown of ISOC1 decreased the ability of cell growth and proliferation in pancreatic cancer cells. Furthermore, Annexin V staining and caspase‑3/7 activity assays demonstrated that inhibition of ISOC1 promoted cell apoptosis via elevation of the expression of caspase‑3/7. Furthermore, inhibition of ISOC1 impaired the cell migration and invasive capability of the cells. In conclusion, ISOC1 exerts a role in pancreatic cancer cell growth and apoptosis, and may have a role in pancreatic cancer tumorigenesis.

View Figures

View References

1	American Cancer Society: Cancer Facts & Figures 2017. American Cancer Society. (Atlantla, GA). 2017.
2	Conroy T, Desseigne F, Ychou M, Bouché O, Guimbaud R, Bécouarn Y, Adenis A, Raoul JL, Gourgou-Bourgade S, de la Fouchardière C, et al: FOLFIRINOX versus gemcitabine for metastatic pancreatic cancer. N Eng J Med. 364:1817–1825. 2011. View Article : Google Scholar
3	Hidalgo M, Cascinu S, Kleeff J, Labianca R, Löhr JM, Neoptolemos J, Real FX, Van Laethem JL and Heinemann V: Addressing the challenges of pancreatic cancer: Future directions for improving outcomes. Pancreatology. 15:8–18. 2015. View Article : Google Scholar : PubMed/NCBI
4	Walsh CT, Liu J, Rusnak F and Sakaitani M: Molecular studies on enzymes in chorismate metabolism and the enterobactin biosynthetic-pathway. Chem Rev. 90:1105–1129. 1990. View Article : Google Scholar
5	Gronemeyer T, Wiese S, Ofman R, Bunse C, Pawlas M, Hayen H, Eisenacher M, Stephan C, Meyer HE, Waterham HR, et al: The proteome of human liver peroxisomes: Identification of five new peroxisomal constituents by a label-free quantitative proteomics survey. PLoS One. 8:e573952013. View Article : Google Scholar : PubMed/NCBI
6	Pedersen CC, Refsgaard JC, Ostergaard O, Jensen LJ, Heegaard NH, Borregaard N and Cowland JB: Impact of microRNA-130a on the neutrophil proteome. BMC Immunol. 16:702015. View Article : Google Scholar : PubMed/NCBI
7	Yamaga R, Ikeda K, Boele J, Horie-Inoue K, Takayama K, Urano T, Kaida K, Carninci P, Kawai J, Hayashizaki Y, et al: Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping. Biochem Biophys Res Commun. 447:531–536. 2014. View Article : Google Scholar : PubMed/NCBI
8	Rezaul K, Thumar JK, Lundgren DH, Eng JK, Claffey KP, Wilson L and Han DK: Differential protein expression profiles in estrogen receptor-positive and -negative breast cancer tissues using label-free quantitative proteomics. Genes Cancer. 1:251–271. 2010. View Article : Google Scholar : PubMed/NCBI
9	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
10	Fischer JJ, Michaelis S, Schrey AK, Diehl A, Graebner OY, Ungewiss J, Horzowski S, Glinski M, Kroll F, Dreger M and Koester H: SAHA capture compound-A novel tool for the profiling of histone deacetylases and the identification of additional vorinostat binders. Proteomics. 11:4096–4104. 2011. View Article : Google Scholar : PubMed/NCBI
11	Hubrich F, Mordhorst S and Andexer JN: Cinnamic acid derivatives as inhibitors for chorismatases and isochorismatases. Bioorg Med Chem Lett. 23:1477–1481. 2013. View Article : Google Scholar : PubMed/NCBI
12	Huang XY, Shi ZC, Wang W, Bai J, Chen Z, Xu J, Zhang D and Fu S: Identification and characterization of a novel protein ISOC2 that interacts with p16(INK4a). Biochem Biophys Res Commun. 361:287–293. 2007. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Knockdown of ISOC1 suppresses cell proliferation in pancreatic cancer in vitro

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles