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Article

Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues

  • Authors:
    • Hak Hoon Jun
    • Kyubum Kwack
    • Keun Hee Lee
    • Jung Oh Kim
    • Han Sung Park
    • Chang Soo Ryu
    • Jeong Yong Lee
    • Daeun Ko
    • Jong Woo Kim
    • Nam Keun Kim
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, CHA Bundang Medical Center, School of Medicine, CHA University, Seongnam 13496, Republic of Korea, Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Republic of Korea, Department of Anesthesiology and Pain Medicine, CHA Bundang Medical Center, CHA University, Seongnam 13496, Republic of Korea
  • Pages: 4726-4734
    |
    Published online on: March 1, 2019
       https://doi.org/10.3892/ol.2019.10092
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Abstract

Colorectal cancer (CRC) is one of the most common types of cancers, as evidenced by the >1.2 million patient diagnoses and 600,000 mortalities globally each year. Recently, the microRNA (miR/miRNA)‑34 miRNA precursor family was revealed to participate in the tumor protein (TP)‑53 pathway, which is frequently involved in CRC. Furthermore, the expression of miR‑34 is reportedly regulated by DNA methylation. Accordingly, the present study investigated the correlation between the methylation status of miR‑34 miRNAs and miR‑34 expression in paired CRC tumor and normal tissues. The methylation status of miR‑34a and miR‑34b/c was determined using the MethyLight assay, and the expression of miR‑34a and miR‑34b/c in the same paired tissues was analyzed by reverse transcription‑quantitative polymerase chain reaction. The results revealed significantly elevated miR‑34a (P=0.012) and miR‑34b/c (P<0.0001) methylation levels in tumor tissues when compared with normal tissues, whereas only the expression of miR‑34b/c differed (P=0.005) between the paired tissues. In addition, an association between TP53 haplotypes and miR‑34 family expression levels was observed. The miR‑34a methylation levels in the TP53 PIN A1A1 (48.56±36.49) and TP53 MSP GG (49.00±36.44) genotypes were increased in the tumor tissues when compared with normal tissues. In conclusion, it was determined that miR‑34 promoter methylation and TP53 polymorphisms may be associated with CRC pathogenesis.
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Copy and paste a formatted citation
Spandidos Publications style
Jun HH, Kwack K, Lee KH, Kim JO, Park HS, Ryu CS, Lee JY, Ko D, Kim JW, Kim NK, Kim NK, et al: Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues. Oncol Lett 17: 4726-4734, 2019.
APA
Jun, H.H., Kwack, K., Lee, K.H., Kim, J.O., Park, H.S., Ryu, C.S. ... Kim, N.K. (2019). Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues. Oncology Letters, 17, 4726-4734. https://doi.org/10.3892/ol.2019.10092
MLA
Jun, H. H., Kwack, K., Lee, K. H., Kim, J. O., Park, H. S., Ryu, C. S., Lee, J. Y., Ko, D., Kim, J. W., Kim, N. K."Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues". Oncology Letters 17.5 (2019): 4726-4734.
Chicago
Jun, H. H., Kwack, K., Lee, K. H., Kim, J. O., Park, H. S., Ryu, C. S., Lee, J. Y., Ko, D., Kim, J. W., Kim, N. K."Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues". Oncology Letters 17, no. 5 (2019): 4726-4734. https://doi.org/10.3892/ol.2019.10092
Copy and paste a formatted citation
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Spandidos Publications style
Jun HH, Kwack K, Lee KH, Kim JO, Park HS, Ryu CS, Lee JY, Ko D, Kim JW, Kim NK, Kim NK, et al: Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues. Oncol Lett 17: 4726-4734, 2019.
APA
Jun, H.H., Kwack, K., Lee, K.H., Kim, J.O., Park, H.S., Ryu, C.S. ... Kim, N.K. (2019). Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues. Oncology Letters, 17, 4726-4734. https://doi.org/10.3892/ol.2019.10092
MLA
Jun, H. H., Kwack, K., Lee, K. H., Kim, J. O., Park, H. S., Ryu, C. S., Lee, J. Y., Ko, D., Kim, J. W., Kim, N. K."Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues". Oncology Letters 17.5 (2019): 4726-4734.
Chicago
Jun, H. H., Kwack, K., Lee, K. H., Kim, J. O., Park, H. S., Ryu, C. S., Lee, J. Y., Ko, D., Kim, J. W., Kim, N. K."Association between TP53 genetic polymorphisms and the methylation and expression of miR‑34a, 34b/c in colorectal cancer tissues". Oncology Letters 17, no. 5 (2019): 4726-4734. https://doi.org/10.3892/ol.2019.10092
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