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Article Open Access

Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression

  • Authors:
    • Aggelis Stavropoulos
    • Michail Varras
    • Thivi Vasilakaki
    • Viktoria‑Konstantina Varra
    • Aikaterini Tsavari
    • Fani‑Niki Varra
    • Aphrodite Nonni
    • Nikolaos Kavantzas
    • Andreas C. Lazaris
  • View Affiliations / Copyright

    Affiliations: Fourth Obstetrics and Gynecology Department, ‘Elena Venizelou’ General Hospital, Athens 11521, Greece, Fifth Obstetrics and Gynecology Department, ‘Elena Venizelou’ General Hospital, Athens 11521, Greece, Pathology Department, ‘Tzaneio’ General Hospital, Piraeus 18536, Greece, Pharmacy Department, University of Patras, Patra 26504, Greece, Pharmacy Department, Frederick University, Nicosia 1036, Republic of Cyprus, First Pathology Department, Medical School, National Kapodistrian University, Athens 11527, Greece
    Copyright: © Stavropoulos et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4575-4589
    |
    Published online on: March 1, 2019
       https://doi.org/10.3892/ol.2019.10093
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Abstract

Endometrial carcinoma is a common malignancy of the female genital tract. Alterations in the expression levels of various oncogenes and tumor suppressor genes serve important roles in the carcinogenesis and biological behavior of endometrial carcinoma. The aim of the present study was to evaluate the combination and individual expression of p53 and phosphatase and tensin homolog (PTEN) protein in human endometrial carcinoma. In addition, the correlation of these proteins with clinicopathological parameters was also assessed. Retrospective immunohistochemical analysis of the expression of p53 and PTEN tumor suppressor proteins was conducted in 99 women with endometrial carcinoma. The overall rate of p53 and PTEN positivity was 89 and 77%, respectively, according to the sum of stain intensity and scores of immunopositive cells. The sum of p53 positivity correlated strongly with PTEN expression (ρ=0.256; P=0.044). The concomitant sum of p53 and PTEN expression was identified in 45% of patients with endometrial adenocarcinoma. Notably, the sum of the immunohistochemical expression of p53 was significantly correlated with patient age (P=0.037), histologic type (P=0.008), histologic grade (P=0.002) and fallopian and/or ovarian invasion (P=0.014). Furthermore, PTEN expression was associated with myometrial invasion (ρ=‑0.377; P=0.002) and clinical stage (P=0.019). In addition, concomitant p53 and PTEN expression was correlated with patient age (P=0.008) and histologic differentiation (P=0.028). The findings indicated a correlation between the expression of p53 and PTEN in endometrial adenocarcinoma, which suggested an intrinsic association between expression levels of these tumor suppressor genes. The study also suggested that concomitant p53 and PTEN expression contributed in characterizing the tumor behavior of endometrial carcinoma. Taken together, the present study suggested the combined expression of p53 and PTEN in the development of high‑grade endometrial carcinoma in older patients. In addition, the findings indicated activation of different molecular pathways in the tumor progression between low‑grade and high‑grade endometrial carcinomas.
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Copy and paste a formatted citation
Spandidos Publications style
Stavropoulos A, Varras M, Vasilakaki T, Varra VK, Tsavari A, Varra FN, Nonni A, Kavantzas N and Lazaris AC: Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression. Oncol Lett 17: 4575-4589, 2019.
APA
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F. ... Lazaris, A.C. (2019). Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression. Oncology Letters, 17, 4575-4589. https://doi.org/10.3892/ol.2019.10093
MLA
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F., Nonni, A., Kavantzas, N., Lazaris, A. C."Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression". Oncology Letters 17.5 (2019): 4575-4589.
Chicago
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F., Nonni, A., Kavantzas, N., Lazaris, A. C."Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression". Oncology Letters 17, no. 5 (2019): 4575-4589. https://doi.org/10.3892/ol.2019.10093
Copy and paste a formatted citation
x
Spandidos Publications style
Stavropoulos A, Varras M, Vasilakaki T, Varra VK, Tsavari A, Varra FN, Nonni A, Kavantzas N and Lazaris AC: Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression. Oncol Lett 17: 4575-4589, 2019.
APA
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F. ... Lazaris, A.C. (2019). Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression. Oncology Letters, 17, 4575-4589. https://doi.org/10.3892/ol.2019.10093
MLA
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F., Nonni, A., Kavantzas, N., Lazaris, A. C."Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression". Oncology Letters 17.5 (2019): 4575-4589.
Chicago
Stavropoulos, A., Varras, M., Vasilakaki, T., Varra, V., Tsavari, A., Varra, F., Nonni, A., Kavantzas, N., Lazaris, A. C."Expression of p53 and PTEN in human primary endometrial carcinomas: Clinicopathological and immunohistochemical analysis and study of their concomitant expression". Oncology Letters 17, no. 5 (2019): 4575-4589. https://doi.org/10.3892/ol.2019.10093
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