Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
May-2019 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2019 Volume 17 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation

  • Authors:
    • Huimin Lu
    • Dujiang Yang
    • Ling Zhang
    • Shan Lu
    • Jun Ye
    • Mao Li
    • Weiming Hu
  • View Affiliations / Copyright

    Affiliations: Department of Pancreatic Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Lu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4633-4639
    |
    Published online on: March 5, 2019
       https://doi.org/10.3892/ol.2019.10111
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Long intergenic non‑protein coding RNA, p53 induced transcript (Linc‑pint) is a newly identified long non‑coding RNA, which has demonstrated antitumor activities in various types of cancer. The present study aimed to investigate the role of Linc‑pint in pancreatic ductal adenocarcinoma (PDAC). Plasma samples from patients with PDAC, and PDAC and normal cell lines were used in the study. Gene expression was analyzed by reverse transcription‑quantitative polymerase chain reaction. Western blotting was used to assess protein level. Transforming growth factor β1 (TGF‑β1) plasma level was determined by ELISA. Cancer cell proliferation was measured in vitro with the Cell Counting Kit‑8 assy. The results demonstrated that Linc‑pint plasma levels were significantly lower in patients with stage 0‑1 PDAC compared with healthy controls. In addition, Linc‑pint downregulation effectively distinguished patients with PDAC from healthy controls. Linc‑pint and TGF‑β1 plasma levels were positively correlated in patients with PDAC but not in healthy controls. Furthermore, Linc‑pint overexpression upregulated TGF‑β1 expression in PDAC cells but not in normal pancreatic ductal cells; however, exogenous TGF‑β1 exhibited no significant effects on Linc‑pint expression. Linc‑pint overexpression and TGF‑β1 both inhibited PDAC cell proliferation, whereas treatment with a TGF‑β inhibitor reduced their inhibitory effects on cell proliferation. In conclusion, results from the present study suggested that Linc‑pint may inhibit early stage PDAC growth through TGF‑β pathway activation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Tomasetti C, Li L and Vogelstein B: Stem cell divisions, somatic mutations, cancer etiology, and cancer prevention. Science. 355:1330–1334. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Veite-Schmahl MJ, Joesten WC and Kennedy MA: HMGA1 expression levels are elevated in pancreatic intraepithelial neoplasia cells in the Ptf1a-Cre; LSL-KrasG12D transgenic mouse model of pancreatic cancer. Br J Cancer. 117:639–647. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Akhurst RJ and Derynck R: TGF-beta signaling in cancer-a double-edged sword. Trends Cell Biol. 11:S44–S51. 2011. View Article : Google Scholar

5 

Derynck R, Akhurst RJ and Balmain A: TGF-beta signaling in tumor suppression and cancer progression. Nat Genet. 29:117–129. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Hezel AF, Deshpande V, Zimmerman SM, Contino G, Alagesan B, O'Dell MR, Rivera LB, Harper J, Lonning S, Brekken RA and Bardeesy N: TGF-β and αvβ6 integrin act in a common pathway to suppress pancreatic cancer progression. Cancer Res. 72:4840–4845. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Yuan JH, Yang F, Wang F, Ma JZ, Guo YJ, Tao QF, Liu F, Pan W, Wang TT, Zhou CC, et al: A long noncoding RNA activated by TGF-β promotes the invasion-metastasis cascade in hepatocellular carcinoma. Cancer Cell. 25:666–681. 2014. View Article : Google Scholar : PubMed/NCBI

8 

Zhou Q, Chung AC, Huang XR, Dong Y, Yu X and Lan HY: Identification of novel long noncoding RNAs associated with TGF-β/Smad3-mediated renal inflammation and fibrosis by RNA sequencing. Am J Pathol. 184:409–417. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Li L, Zhang GQ, Chen H, Zhao ZJ, Chen HZ, Liu H, Wang G, Jia YH, Pan SH and Kong R: Plasma and tumor levels of Linc-pint are diagnostic and prognostic biomarkers for pancreatic cancer. Oncotarget. 7:71773–71781. 2016.PubMed/NCBI

10 

Marín-Béjar O, Mas AM, González J, Martinez D, Athie A, Morales X, Galduroz M, Raimondi I, Grossi E, Guo S, et al: The human lncRNA LINC-PINT inhibits tumor cell invasion through a highly conserved sequence element. Genome Biol. 18:2022017. View Article : Google Scholar : PubMed/NCBI

11 

Garitano-Trojaola A, San José-Enériz E, Ezponda T, Unfried JP, Carrasco-León A, Razquin N, Barriocanal M, Vilas-Zornoza A, Sangro B, Segura V, et al: Deregulation of linc-PINT in acute lymphoblastic leukemia is implicated in abnormal proliferation of leukemic cells. Oncotarget. 9:12842–12852. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Chun YS, Pawlik TM and Vauthey JN: 8th Edition of the AJCC Cancer Staging Manual: Pancreas and hepatobiliary cancers. Ann Surg Oncol. 25:845–847. 2018. View Article : Google Scholar : PubMed/NCBI

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

14 

Seoane J and Gomis RR: TGF-β family signaling in tumor suppression and cancer progression. Cold Spring Harb Perspect Biol. 9(pii): a0222772017. View Article : Google Scholar : PubMed/NCBI

15 

Shen W, Tao GQ, Zhang Y, Cai B, Sun J and Tian ZQ: TGF-β in pancreatic cancer initiation and progression: Two sides of the same coin. Cell Biosci. 7:392017. View Article : Google Scholar : PubMed/NCBI

16 

Zhang X, Feng W, Zhang J, Ge L, Zhang Y, Jiang X, Peng W, Wang D, Gong A and Xu M: Long non-coding RNA PVT1 promotes epithelial-mesenchymal transition via the TGF-β/Smad pathway in pancreatic cancer cells. Oncol Rep. 40:1093–1102. 2018.PubMed/NCBI

17 

Xing W, Gao W, Mao G, Zhang J, Lv X, Wang G and Yan J: Long non-coding RNAs in aging organs and tissues. Clin Exp Pharmacol Physiol. 44 (Suppl):S30–S37. 2017. View Article : Google Scholar

18 

Chen J, Chen Y, Gu L, Li X, Gao Y, Lyu X, Chen L, Luo G, Wang L, Xie Y, et al: LncRNAs act as prognostic and diagnostic biomarkers in renal cell carcinoma: A systematic review and meta-analysis. Oncotarget. 7:74325–74336. 2016.PubMed/NCBI

19 

Ijiri N, Panariti A, Mogas A, Nair P, Hamid Q, Martin J and Baglole C: Lncrna Neat1 promotes Il-8 expression in fibroblasts derived from COPD patients and in response to cigarette smoking. Am J Respir Crit Care Med. 195:A76762017.https://www.atsjournals.org/doi/abs/10.1164/ajrccm-conference.2017.195.1_MeetingAbstracts.A7676

20 

Gao M, Li H, Lv X, Zhou B and Yin Z: Association between four polymorphisms in lncRNA and risk of lung cancer in a Chinese never-smoking female population. DNA Cell Biol. 37:651–658. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Yu V, Singh P, Rahimy E, Zheng H, Kuo SZ, Kim E, Wang-Rodriguez J and Ongkeko WM: RNA-seq analysis identifies key long non-coding RNAs connected to the pathogenesis of alcohol-associated head and neck squamous cell carcinoma. Oncol Lett. 12:2846–2853. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Morikawa M, Derynck R and Miyazono K: TGF-β and the TGF-β family: Context-dependent roles in cell and tissue physiology. Cold Spring Harb Perspect Biol. 8:a0218732016. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lu H, Yang D, Zhang L, Lu S, Ye J, Li M and Hu W: Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation. Oncol Lett 17: 4633-4639, 2019.
APA
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., & Hu, W. (2019). Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation. Oncology Letters, 17, 4633-4639. https://doi.org/10.3892/ol.2019.10111
MLA
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., Hu, W."Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation". Oncology Letters 17.5 (2019): 4633-4639.
Chicago
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., Hu, W."Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation". Oncology Letters 17, no. 5 (2019): 4633-4639. https://doi.org/10.3892/ol.2019.10111
Copy and paste a formatted citation
x
Spandidos Publications style
Lu H, Yang D, Zhang L, Lu S, Ye J, Li M and Hu W: Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation. Oncol Lett 17: 4633-4639, 2019.
APA
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., & Hu, W. (2019). Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation. Oncology Letters, 17, 4633-4639. https://doi.org/10.3892/ol.2019.10111
MLA
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., Hu, W."Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation". Oncology Letters 17.5 (2019): 4633-4639.
Chicago
Lu, H., Yang, D., Zhang, L., Lu, S., Ye, J., Li, M., Hu, W."Linc‑pint inhibits early stage pancreatic ductal adenocarcinoma growth through TGF‑β pathway activation". Oncology Letters 17, no. 5 (2019): 4633-4639. https://doi.org/10.3892/ol.2019.10111
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team