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Correlation of OGR1 with proliferation and apoptosis of breast cancer cells

  • Authors:
    • Jianguo Zhang
    • Lei Che
    • Wenkai Sun
    • Jian Shang
    • Min Hao
    • Mengzi Tian
  • View Affiliations / Copyright

    Affiliations: Department of Breast and Thyroid Surgery, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China, Department of Anesthesia and Surgery, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4335-4340
    |
    Published online on: March 6, 2019
       https://doi.org/10.3892/ol.2019.10121
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Abstract

Effects of ovarian cancer G-protein-coupled receptor 1 (OGR1) protein on proliferation and apoptosis of breast cancer cells, as well as its molecular mechanism were investigated. The MCF-7 cell line highly expressed OGR1 was constructed by transient transfection of eukaryotic expression vector using breast cancer cells. At the same time, cells were transfected with empty vector as controls. The effects of highly expressed OGR1 on cell growth, proliferation, apoptosis and other abilities were identified. In addition, the effects of highly expressed OGR1 on serine-threonine kinase (AKT), p53 and other genes were studied. It was proved in apoptosis experiment that highly expressed OGR1 protein in breast cancer cells could effectively increase the proportion of apoptosis of cells. Cell proliferation experiment revealed that the growth and proliferation abilities of breast cancer cells with highly expressed OGR1 were inhibited to some extent, compared with those of breast cancer cells with low expression of OGR1. Results of western blotting showed that the gene and protein expression levels of p53 in breast cancer cells with highly expressed OGR1 were increased. There was no significant difference in protein expression of AKT between breast cancer cells with low expression of OGR1 and those with highly expressed OGR1. However, the protein content of phosphorylated-AKT (p-AKT) in breast cancer cells with highly expressed OGR1 was lower than that in breast cancer cells with low expression of OGR1. The proliferation and apoptosis of breast cancer cells are influenced by the changes of OGR1 expression, which are correlated with the gene expression levels of AKT and p53 to some extent, but the detailed molecular mechanism requires additional study.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Che L, Sun W, Shang J, Hao M and Tian M: Correlation of OGR1 with proliferation and apoptosis of breast cancer cells. Oncol Lett 17: 4335-4340, 2019.
APA
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., & Tian, M. (2019). Correlation of OGR1 with proliferation and apoptosis of breast cancer cells. Oncology Letters, 17, 4335-4340. https://doi.org/10.3892/ol.2019.10121
MLA
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., Tian, M."Correlation of OGR1 with proliferation and apoptosis of breast cancer cells". Oncology Letters 17.5 (2019): 4335-4340.
Chicago
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., Tian, M."Correlation of OGR1 with proliferation and apoptosis of breast cancer cells". Oncology Letters 17, no. 5 (2019): 4335-4340. https://doi.org/10.3892/ol.2019.10121
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Che L, Sun W, Shang J, Hao M and Tian M: Correlation of OGR1 with proliferation and apoptosis of breast cancer cells. Oncol Lett 17: 4335-4340, 2019.
APA
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., & Tian, M. (2019). Correlation of OGR1 with proliferation and apoptosis of breast cancer cells. Oncology Letters, 17, 4335-4340. https://doi.org/10.3892/ol.2019.10121
MLA
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., Tian, M."Correlation of OGR1 with proliferation and apoptosis of breast cancer cells". Oncology Letters 17.5 (2019): 4335-4340.
Chicago
Zhang, J., Che, L., Sun, W., Shang, J., Hao, M., Tian, M."Correlation of OGR1 with proliferation and apoptosis of breast cancer cells". Oncology Letters 17, no. 5 (2019): 4335-4340. https://doi.org/10.3892/ol.2019.10121
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