Correlation of OGR1 with proliferation and apoptosis of breast cancer cells

Zhang,Jianguo; Che,Lei; Sun,Wenkai; Shang,Jian; Hao,Min; Tian,Mengzi

doi:10.3892/ol.2019.10121

Correlation of OGR1 with proliferation and apoptosis of breast cancer cells

Authors:
- Jianguo Zhang
- Lei Che
- Wenkai Sun
- Jian Shang
- Min Hao
- Mengzi Tian
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Affiliations: Department of Breast and Thyroid Surgery, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China, Department of Anesthesia and Surgery, Dongying People's Hospital, Dongying, Shandong 257091, P.R. China
Published online on: March 6, 2019 https://doi.org/10.3892/ol.2019.10121
Pages: 4335-4340
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Effects of ovarian cancer G-protein-coupled receptor 1 (OGR1) protein on proliferation and apoptosis of breast cancer cells, as well as its molecular mechanism were investigated. The MCF-7 cell line highly expressed OGR1 was constructed by transient transfection of eukaryotic expression vector using breast cancer cells. At the same time, cells were transfected with empty vector as controls. The effects of highly expressed OGR1 on cell growth, proliferation, apoptosis and other abilities were identified. In addition, the effects of highly expressed OGR1 on serine-threonine kinase (AKT), p53 and other genes were studied. It was proved in apoptosis experiment that highly expressed OGR1 protein in breast cancer cells could effectively increase the proportion of apoptosis of cells. Cell proliferation experiment revealed that the growth and proliferation abilities of breast cancer cells with highly expressed OGR1 were inhibited to some extent, compared with those of breast cancer cells with low expression of OGR1. Results of western blotting showed that the gene and protein expression levels of p53 in breast cancer cells with highly expressed OGR1 were increased. There was no significant difference in protein expression of AKT between breast cancer cells with low expression of OGR1 and those with highly expressed OGR1. However, the protein content of phosphorylated-AKT (p-AKT) in breast cancer cells with highly expressed OGR1 was lower than that in breast cancer cells with low expression of OGR1. The proliferation and apoptosis of breast cancer cells are influenced by the changes of OGR1 expression, which are correlated with the gene expression levels of AKT and p53 to some extent, but the detailed molecular mechanism requires additional study.

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1	Sirkisoon SR, Carpenter RL, Rimkus T, Miller L, Metheny-Barlow L and Lo HW: EGFR and HER2 signaling in breast cancer brain metastasis. Front Biosci (Elite Ed). 8:245–263. 2016.PubMed/NCBI
2	Siegel RL, Miller KD and Jemal A: Cancer statistics, 2015. CA Cancer J Clin. 65:5–29. 2015. View Article : Google Scholar : PubMed/NCBI
3	Fidler IJ: The pathogenesis of cancer metastasis: The ‘seed and soil’ hypothesis revisited. Nat Rev Cancer. 3:453–458. 2003. View Article : Google Scholar : PubMed/NCBI
4	Nola S, Sin S, Bonin F, Lidereau R and Driouch K: A methodological approach to unravel organ-specific breast cancer metastasis. J Mammary Gland Biol Neoplasia. 17:135–145. 2012. View Article : Google Scholar : PubMed/NCBI
5	O'Shaughnessy J: Extending survival with chemotherapy in metastatic breast cancer. Oncologist. 10 (Suppl 3):20–29. 2005. View Article : Google Scholar : PubMed/NCBI
6	Shao MM, Liu J, Vong JS, Niu Y, Germin B, Tang P, Chan AW, Lui PC, Law BK, Tan PH, et al: A subset of breast cancer predisposes to brain metastasis. Med Mol Morphol. 44:15–20. 2011. View Article : Google Scholar : PubMed/NCBI
7	Hohensee I, Lamszus K, Riethdorf S, Meyer-Staeckling S, Glatzel M, Matschke J, Witzel I, Westphal M, Brandt B, Müller V, et al: Frequent genetic alterations in EGFR- and HER2-driven pathways in breast cancer brain metastases. Am J Pathol. 183:83–95. 2013. View Article : Google Scholar : PubMed/NCBI
8	Aragon-Ching JB and Zujewski JA: CNS metastasis: An old problem in a new guise. Clin Cancer Res. 13:1644–1647. 2007. View Article : Google Scholar : PubMed/NCBI
9	Brufsky AM, Mayer M, Rugo HS, Kaufman PA, Tan-Chiu E, Tripathy D, Tudor IC, Wang LI, Brammer MG, Shing M, et al: Central nervous system metastases in patients with HER2-positive metastatic breast cancer: Incidence, treatment, and survival in patients from registHER. Clin Cancer Res. 17:4834–4843. 2011. View Article : Google Scholar : PubMed/NCBI
10	Klein A, Olendrowitz C, Schmutzler R, Hampl J, Schlag PM, Maass N, Arnold N, Wessel R, Ramser J, Meindl A, et al: Identification of brain- and bone-specific breast cancer metastasis genes. Cancer Lett. 276:212–220. 2009. View Article : Google Scholar : PubMed/NCBI
11	Niikura N, Hayashi N, Masuda N, Takashima S, Nakamura R, Watanabe K, Kanbayashi C, Ishida M, Hozumi Y, Tsuneizumi M, et al: Treatment outcomes and prognostic factors for patients with brain metastases from breast cancer of each subtype: A multicenter retrospective analysis. Breast Cancer Res Treat. 147:103–112. 2014. View Article : Google Scholar : PubMed/NCBI
12	Ludwig MG, Vanek M, Guerini D, Gasser JA, Jones CE, Junker U, Hofstetter H, Wolf RM and Seuwen K: Proton-sensing G-protein-coupled receptors. Nature. 425:93–98. 2003. View Article : Google Scholar : PubMed/NCBI
13	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
14	Yan L, Chang Z, Liu Y, He BR and Hao DJ: Primary spinal melanoma: A case report and literature review. Chin Med J (Engl). 125:4138–4141. 2012.PubMed/NCBI
15	Yan L, Singh LS, Zhang L and Xu Y: Role of OGR1 in myeloid-derived cells in prostate cancer. Oncogene. 33:157–164. 2014. View Article : Google Scholar : PubMed/NCBI
16	Li J, Guo B, Wang J, Cheng X, Xu Y and Sang J: Ovarian cancer G protein coupled receptor 1 suppresses cell migration of MCF7 breast cancer cells via a Gα12/13-Rho-Rac1 pathway. J Mol Signal. 8:62013. View Article : Google Scholar : PubMed/NCBI
17	Yuan FL, Wang HR, Zhao MD, Yuan W, Cao L, Duan PG, Jiang YQ, Li XL and Dong J: Ovarian cancer G protein-coupled receptor 1 is involved in acid-induced apoptosis of endplate chondrocytes in intervertebral discs. J Bone Miner Res. 29:67–77. 2014. View Article : Google Scholar : PubMed/NCBI
18	Datta SR, Brunet A and Greenberg ME: Cellular survival: A play in three Akts. Genes Dev. 13:2905–2927. 1999. View Article : Google Scholar : PubMed/NCBI
19	Muise-Helmericks RC, Grimes HL, Bellacosa A, Malstrom SE, Tsichlis PN and Rosen N: Cyclin D expression is controlled post-transcriptionally via a phosphatidylinositol 3-kinase/Akt-dependent pathway. J Biol Chem. 273:29864–29872. 1998. View Article : Google Scholar : PubMed/NCBI
20	Mayo LD and Donner DB: A phosphatidylinositol 3-kinase/Akt pathway promotes translocation of Mdm2 from the cytoplasm to the nucleus. Proc Natl Acad Sci USA. 98:11598–11603. 2001. View Article : Google Scholar : PubMed/NCBI
21	Michell BJ, Griffiths JE, Mitchelhill KI, Rodriguez-Crespo I, Tiganis T, Bozinovski S, de Montellano PR, Kemp BE and Pearson RB: The Akt kinase signals directly to endothelial nitric oxide synthase. Curr Biol. 9:845–848. 1999. View Article : Google Scholar : PubMed/NCBI
22	Hurt KJ, Musicki B, Palese MA, Crone JK, Becker RE, Moriarity JL, Snyder SH and Burnett AL: Akt-dependent phosphorylation of endothelial nitric-oxide synthase mediates penile erection. Proc Natl Acad Sci USA. 99:4061–4066. 2002. View Article : Google Scholar : PubMed/NCBI
23	Kim D, Kim S, Koh H, Yoon SO, Chung AS, Cho KS and Chung J: Akt/PKB promotes cancer cell invasion via increased motility and metalloproteinase production. FASEB J. 15:1953–1962. 2001. View Article : Google Scholar : PubMed/NCBI
24	Kubiatowski T, Jang T, Lachyankar MB, Salmonsen R, Nabi RR, Quesenberry PJ, Litofsky NS, Ross AH and Recht LD: Association of increased phosphatidylinositol 3-kinase signaling with increased invasiveness and gelatinase activity in malignant gliomas. J Neurosurg. 95:480–488. 2001. View Article : Google Scholar : PubMed/NCBI
25	Iremashvili V, Burdick-Will J and Soloway MS: Improving risk stratification in patients with prostate cancer managed by active surveillance: A nomogram predicting the risk of biopsy progression. BJU Int. 112:39–44. 2013. View Article : Google Scholar : PubMed/NCBI