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Article Open Access

RANBP9 suppresses tumor proliferation in colorectal cancer

  • Authors:
    • Chunzhi Qin
    • Qin Zhang
    • Guangbin Wu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China
    Copyright: © Qin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4409-4416
    |
    Published online on: March 8, 2019
       https://doi.org/10.3892/ol.2019.10134
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Abstract

RAN binding protein 9 (RANBP9) is widely expressed in mammalian tissues, including osteosarcoma, lung, gastric and breast cancer tissues. However, currently, not much is known about the role of RANBP9 in colorectal cancer (CRC). In the present study, RANBP9 expression in CRC tissues and cell lines was measured by immunohistochemistry and western blotting, respectively. Subsequently, RANBP9‑short hairpin RNA (shRNA) and RANBP9 plasmids were constructed and transfected into HCT116 and HT29 cells. The effects of RANBP9 knockdown were assessed by Cell Counting kit‑8 and colony formation assays, and its effects on tumorigenicity in a nude mouse animal model were investigated. The effect of RANBP9‑shRNA on cell cycle progression was analyzed by flow cytometry, while cell cycle‑associated protein expression levels were examined by western blotting. Compared with in paired normal mucosa, RANBP9 was overexpressed in CRC tissues. Inhibition of RANBP9 in HCT116 and HT29 cells significantly promoted cell growth, colony formation and S phase transition, and increased tumorigenesis in vivo. Accordingly, RANBP9 overexpression inhibited cell growth and colony formation. Knockdown of RANBP9 was associated with upregulated cyclin A2 in the two cell lines. In conclusion, RANBP9 served an inhibitory role in CRC in vitro and in vivo. Therefore, RANBP9 may be considered a potential target for treatment of CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Qin C, Zhang Q and Wu G: RANBP9 suppresses tumor proliferation in colorectal cancer. Oncol Lett 17: 4409-4416, 2019.
APA
Qin, C., Zhang, Q., & Wu, G. (2019). RANBP9 suppresses tumor proliferation in colorectal cancer. Oncology Letters, 17, 4409-4416. https://doi.org/10.3892/ol.2019.10134
MLA
Qin, C., Zhang, Q., Wu, G."RANBP9 suppresses tumor proliferation in colorectal cancer". Oncology Letters 17.5 (2019): 4409-4416.
Chicago
Qin, C., Zhang, Q., Wu, G."RANBP9 suppresses tumor proliferation in colorectal cancer". Oncology Letters 17, no. 5 (2019): 4409-4416. https://doi.org/10.3892/ol.2019.10134
Copy and paste a formatted citation
x
Spandidos Publications style
Qin C, Zhang Q and Wu G: RANBP9 suppresses tumor proliferation in colorectal cancer. Oncol Lett 17: 4409-4416, 2019.
APA
Qin, C., Zhang, Q., & Wu, G. (2019). RANBP9 suppresses tumor proliferation in colorectal cancer. Oncology Letters, 17, 4409-4416. https://doi.org/10.3892/ol.2019.10134
MLA
Qin, C., Zhang, Q., Wu, G."RANBP9 suppresses tumor proliferation in colorectal cancer". Oncology Letters 17.5 (2019): 4409-4416.
Chicago
Qin, C., Zhang, Q., Wu, G."RANBP9 suppresses tumor proliferation in colorectal cancer". Oncology Letters 17, no. 5 (2019): 4409-4416. https://doi.org/10.3892/ol.2019.10134
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