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Article Open Access

Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells

  • Authors:
    • Min Xiang
    • Wuxiong Yuan
    • Weiwei Zhang
    • Jie Huang
  • View Affiliations / Copyright

    Affiliations: Department of Urinary Surgery, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China, Department of Chinese Medicine, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China, Department of Emergency, Hunan Provincial People's Hospital, Changsha, Hunan 410005, P.R. China
    Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4437-4442
    |
    Published online on: March 12, 2019
       https://doi.org/10.3892/ol.2019.10143
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Abstract

Changes in the expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer tissues were studied. A total of 30 patients with bladder cancer who had surgical resection in the Hunan Provincial People's Hospital (Changsha, China) from April 2015 to August 2016 were selected. RT‑qPCR was used to detect the expression levels of miR‑490‑5p, miR‑148a‑3p and miR‑608. The expression vectors of miR‑490‑5p, miR‑148a‑3p and miR‑608 were respectively transfected and divided into three groups: blank cell group, gene transfection group (groups A‑C) and negative transfection group (NC group). CCK8 was used to detect cell proliferation and flow cytometry was used to detect the condition of apoptosis of each group, and the Transwell chamber was used to detect the invasion ability of the cells. After the transfection, the expression level of miR‑490‑5p in group A was significantly higher than that in the NC and blank groups, and the expression level of miR‑148a‑3p in group B was significantly higher than that in the NC and blank groups. The expression level of miR‑608 in group C was significantly higher than that in the NC and blank groups (P<0.001). The survival rates of the cells in groups A‑C were significantly lower than those in the NC and blank groups at 48 and 72 h (P<0.001). After the transfection, the number of invasive cells and the apoptosis rates in groups A‑C were significantly higher than those in the NC and blank groups (P<0.05). miR‑490‑5p, miR‑148a‑3p and miR‑608 promoted proliferation of bladder cancer T24 cells and inhibited apoptosis of the cells and showed potential to become a new target for the future treatment of bladder cancer.
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1 

Grayson M: Bladder cancer. Nature. 551:S332017. View Article : Google Scholar : PubMed/NCBI

2 

Antoni S, Ferlay J, Soerjomataram I, Znaor A, Jemal A and Bray F: Bladder cancer incidence and mortality: A global overview and recent trends. Eur Urol. 71:96–108. 2017. View Article : Google Scholar : PubMed/NCBI

3 

Tuccori M, Filion KB, Yin H, Yu OH, Platt RW and Azoulay L: Pioglitazone use and risk of bladder cancer: Population based cohort study. BMJ. 352:i15412016. View Article : Google Scholar : PubMed/NCBI

4 

Sato Y, Kondo T, Takagi T, Junpei I and Tanabe K: Treatment strategy for bladder cancer in patients on hemodialysis: A clinical review of 28 cases. Int Urol Nephrol. 48:503–509. 2016. View Article : Google Scholar : PubMed/NCBI

5 

Yang GL, Zhang LH, Liu Q, Wang ZL, Duan XH, Huang YR and Bo JJ: Commentary on ‘A novel treatment strategy for newly diagnosed high-grade T1 bladder cancer: gemcitabine and cisplatin adjuvant chemotherapy-A single-institution experience’. Urol Oncol. 36:346–347. 2018. View Article : Google Scholar : PubMed/NCBI

6 

Heidari F, Abbas Zade S, Mir Hosseini SH and Ghadian A: Metformin for the prevention of bladder cancer recurrence: Is it effective? Nephrourol Mon. 8:e302612016. View Article : Google Scholar : PubMed/NCBI

7 

Parodi A, Traverso P, Kalli F, Conteduca G, Tardito S, Curto M, Grillo F, Mastracci L, Bernardi C, Nasi G, et al: Residual tumor micro-foci and overwhelming regulatory T lymphocyte infiltration are the causes of bladder cancer recurrence. Oncotarget. 7:6424–6435. 2016. View Article : Google Scholar : PubMed/NCBI

8 

Mearini E, Poli G, Cochetti G, Boni A, Egidi MG and Brancorsini S: Expression of urinary miRNAs targeting NLRs inflammasomes in bladder cancer. OncoTargets Ther. 10:2665–2673. 2017. View Article : Google Scholar

9 

Wang C, Chen Z, Ge Q, Hu J, Li F, Hu J, Xu H, Ye Z and Li LC: Up-regulation of p21(WAF1/CIP1) by miRNAs and its implications in bladder cancer cells. FEBS Lett. 588:4654–4664. 2014. View Article : Google Scholar : PubMed/NCBI

10 

Lan G, Yang L, Xie X, Peng L and Wang Y: MicroRNA-490-5p is a novel tumor suppressor targeting c-FOS in human bladder cancer. Arch Med Sci. 11:561–569. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Wang X, Liang Z, Xu X, Li J, Zhu Y, Meng S, Li S, Wang S, Xie B, Ji A, et al: miR-148a-3p represses proliferation and EMT by establishing regulatory circuits between ERBB3/AKT2/c-myc and DNMT1 in bladder cancer. Cell Death Dis. 7:e25032016. View Article : Google Scholar : PubMed/NCBI

12 

Liang Z, Wang X, Xu X, Xie B, Ji A, Meng S, Li S, Zhu Y, Wu J, Hu Z, et al: MicroRNA-608 inhibits proliferation of bladder cancer via AKT/FOXO3a signaling pathway. Mol Cancer. 16:962017. View Article : Google Scholar : PubMed/NCBI

13 

Zhao F, Ge YZ, Zhou LH, Xu LW, Xu Z, Ping WW, Wang M, Zhou CC, Wu R and Jia RP: Identification of hub miRNA biomarkers for bladder cancer by weighted gene coexpression network analysis. OncoTargets Ther. 10:5551–5559. 2017. View Article : Google Scholar

14 

Wang Y, Du C, Zhang N, Li M, Liu Y, Zhao M, Wang F and Luo F: TGF-β1 mediates the effects of aspirin on colonic tumor cell proliferation and apoptosis. Oncol Lett. 15:5903–5909. 2018.PubMed/NCBI

15 

Chen W, Wang J, Liu S, Wang S, Cheng Y, Zhou W, Duan C and Zhang C: MicroRNA-361-3p suppresses tumor cell proliferation and metastasis by directly targeting SH2B1 in NSCLC. J Exp Clin Cancer Res. 35:762016. View Article : Google Scholar : PubMed/NCBI

16 

Wen Z, Zhang Y, Wang X, Zeng X, Hu Z, Liu Y, Xie Y, Liang G, Zhu J, Luo H, et al: Novel 3′,5′-diprenylated chalcones inhibited the proliferation of cancer cells in vitro by inducing cell apoptosis and arresting cell cycle phase. Eur J Med Chem. 133:227–239. 2017. View Article : Google Scholar : PubMed/NCBI

17 

Yano S, Mii S, Tome Y, Hiroshima Y, Uehara F, Miwa S and Hoffman RM: Abstract A41: Cancer cells invade or divide, not both. Cancer Res. 73 Suppl 3:A412013. View Article : Google Scholar

18 

Baumgart S, Hölters S, Ohlmann CH, Bohle R, Stöckle M, Ostenfeld MS, Dyrskjøt L, Junker K and Heinzelmann J: Exosomes of invasive urothelial carcinoma cells are characterized by a specific miRNA expression signature. Oncotarget. 8:58278–58291. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Chen W, Ye L, Wen D and Chen F: MiR-490-5p inhibits hepatocellular carcinoma cell proliferation, migration and invasion by directly regulating ROBO1. Pathol Oncol Res. 2:1–9. 2017.

20 

Othman N, In LL, Harikrishna JA and Hasima N: Bcl-xL silencing induces alterations in hsa-miR-608 expression and subsequent cell death in A549 and SK-LU1 human lung adenocarcinoma cells. PLoS One. 8:e817352013. View Article : Google Scholar : PubMed/NCBI

21 

Lombard A, Mooso B, Libertini S, Lim R, Costanzo N, Ghosh P and Mudryj M: Abstract 5239: MiR-148a promotes apoptosis in urothelial cell carcinoma of the bladder cells in part by targeting DNMT1. Cancer Res. 74 Suppl 19:52392014. View Article : Google Scholar

22 

He L, Meng D, Zhang SH, Zhang Y, Deng Z and Kong LB: microRNA-608 inhibits human hepatocellular carcinoma cell proliferation via targeting the BET family protein BRD4. Biochem Biophys Res Commun. 501:1060–1067. 2018. View Article : Google Scholar : PubMed/NCBI

23 

Li S, Xu X, Xu X, Hu Z, Wu J, Zhu Y, Chen H, Mao Y, Lin Y, Luo J, et al: MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos. Biochem Biophys Res Commun. 441:976–981. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Mao LM, Chen YH and Liao ZP: The abnormal expression of miR-148a in bladder cancer and its clinical significance. Chin J Health Lab Tech. 15:2143–2145. 2017.(In Chinese).

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Copy and paste a formatted citation
Spandidos Publications style
Xiang M, Yuan W, Zhang W and Huang J: Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells. Oncol Lett 17: 4437-4442, 2019.
APA
Xiang, M., Yuan, W., Zhang, W., & Huang, J. (2019). Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells. Oncology Letters, 17, 4437-4442. https://doi.org/10.3892/ol.2019.10143
MLA
Xiang, M., Yuan, W., Zhang, W., Huang, J."Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells". Oncology Letters 17.5 (2019): 4437-4442.
Chicago
Xiang, M., Yuan, W., Zhang, W., Huang, J."Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells". Oncology Letters 17, no. 5 (2019): 4437-4442. https://doi.org/10.3892/ol.2019.10143
Copy and paste a formatted citation
x
Spandidos Publications style
Xiang M, Yuan W, Zhang W and Huang J: Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells. Oncol Lett 17: 4437-4442, 2019.
APA
Xiang, M., Yuan, W., Zhang, W., & Huang, J. (2019). Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells. Oncology Letters, 17, 4437-4442. https://doi.org/10.3892/ol.2019.10143
MLA
Xiang, M., Yuan, W., Zhang, W., Huang, J."Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells". Oncology Letters 17.5 (2019): 4437-4442.
Chicago
Xiang, M., Yuan, W., Zhang, W., Huang, J."Expression of miR‑490‑5p, miR‑148a‑3p and miR‑608 in bladder cancer and their effects on the biological characteristics of bladder cancer cells". Oncology Letters 17, no. 5 (2019): 4437-4442. https://doi.org/10.3892/ol.2019.10143
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