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Article Open Access

MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway

  • Authors:
    • Qingla Li
    • Yingnan Chang
    • Luyan Mu
    • Yuwen Song
  • View Affiliations / Copyright

    Affiliations: Second Department of Minimal Invasive Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, First Department of Neurosurgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4819-4826
    |
    Published online on: March 18, 2019
       https://doi.org/10.3892/ol.2019.10158
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Abstract

Glioma is the most common primary tumor of the central nervous system (CNS) that develops chemotherapy resistance. The microRNA (miRNA) miR‑9 is a tissue‑specific miRNA of the CNS that may serve a key role in the modulation of chemotherapy sensitivity. The aim of the present study was to investigate the effect of miR‑9 on glioma chemotherapy sensitivity by altering the expression of miR‑9 in U251 glioma cells by viral transfection and subsequently treating with gradient concentrations of temozolomide (TMZ). Cell viability, apoptosis and the cell cycle were examined, and drug resistance genes were analyzed by western blotting. The role of nuclear factor κB (NF‑κB) in this regulation was also examined. The results revealed that the susceptibility of glioma cells to TMZ was enhanced by miR‑9 overexpression. When miR‑9 and TMZ were applied together, the apoptotic rate and percentage of cells arrested at the G2/M stage were significantly higher compared with either treatment alone. Topoisomerase II expression was suppressed by miR‑9 via the NF‑κB signaling pathway, which may be responsible for the sensitization. The results of the present study suggested that miR‑9 may be a potential target for glioma chemotherapy.
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Copy and paste a formatted citation
Spandidos Publications style
Li Q, Chang Y, Mu L and Song Y: MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway. Oncol Lett 17: 4819-4826, 2019.
APA
Li, Q., Chang, Y., Mu, L., & Song, Y. (2019). MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway. Oncology Letters, 17, 4819-4826. https://doi.org/10.3892/ol.2019.10158
MLA
Li, Q., Chang, Y., Mu, L., Song, Y."MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway". Oncology Letters 17.6 (2019): 4819-4826.
Chicago
Li, Q., Chang, Y., Mu, L., Song, Y."MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway". Oncology Letters 17, no. 6 (2019): 4819-4826. https://doi.org/10.3892/ol.2019.10158
Copy and paste a formatted citation
x
Spandidos Publications style
Li Q, Chang Y, Mu L and Song Y: MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway. Oncol Lett 17: 4819-4826, 2019.
APA
Li, Q., Chang, Y., Mu, L., & Song, Y. (2019). MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway. Oncology Letters, 17, 4819-4826. https://doi.org/10.3892/ol.2019.10158
MLA
Li, Q., Chang, Y., Mu, L., Song, Y."MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway". Oncology Letters 17.6 (2019): 4819-4826.
Chicago
Li, Q., Chang, Y., Mu, L., Song, Y."MicroRNA‑9 enhances chemotherapy sensitivity of glioma to TMZ by suppressing TOPO II via the NF‑κB signaling pathway". Oncology Letters 17, no. 6 (2019): 4819-4826. https://doi.org/10.3892/ol.2019.10158
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