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Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells

  • Authors:
    • Yibiao Ye
    • Yaorong Peng
    • Yanshan Li
    • Chunxia Liu
    • Yunxiuxiu Xu
    • Wenbin Li
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Biliopancreatic Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Key Laboratory of Malignant Tumor Gene Regulation and Target Therapy of Guangdong Higher Education Institutes, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Ultrasound, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510120, P.R. China
    Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4964-4968
    |
    Published online on: March 28, 2019
       https://doi.org/10.3892/ol.2019.10195
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Abstract

The effects of long intergenic non-coding ribonucleic acid (lincRNA)-p21 targeting hypoxia-inducible factor-1α (HIF-1α) on proliferation, apoptosis and migration of liver cancer cells were investigated. MHCC97H liver cancer cells were infected with control lentivirus (control group) and lincRNA-p21 lentivirus (observation group), and control stable cell lines and lincRNA-p21 stable cell lines were screened and obtained by using puromycin. The expression levels of lincRNA-p21 messenger RNA (mRNA) in the control and observation groups were analyzed via reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Bioinformatics was used to search for the lincRNA-p21 target. The expression of target gene was analyzed via western blotting, and the proliferation, apoptosis, migration and in vivo tumor formation of MHCC97H cells in the control and observation groups were also analyzed. Compared with that in control group, the lincRNA-p21 mRNA level in observation group was increased significantly (P<0.05). It was found via bioinformatic comparison that HIF-1α was one of the targets of lincRNA-p21. Results of Western blotting revealed that the expression level of HIF-1α protein in cells in observation group was significantly downregulated (P<0.05). Besides, the level of vascular endothelial growth factor (VEGF) protein in cells in control group was obviously higher than that in observation group (P<0.05). Compared with those in control group, the cell proliferation and migration capacities in observation group were markedly reduced, but the apoptosis level was significantly increased (P<0.05). According to the in vivo tumor formation assay, the cell proliferation rate in control group was obviously higher than that in observation group (P<0.05). The number of tumor blood vessels in cells in control group was obviously reduced compared with that in observation group (P<0.05). lincRNA-p21 can significantly downregulate the level of HIF-1α, thus downregulating the expression of VEGF and affecting the cell proliferation, apoptosis and migration.
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Copy and paste a formatted citation
Spandidos Publications style
Ye Y, Peng Y, Li Y, Liu C, Xu Y and Li W: Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells . Oncol Lett 17: 4964-4968, 2019.
APA
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., & Li, W. (2019). Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells . Oncology Letters, 17, 4964-4968. https://doi.org/10.3892/ol.2019.10195
MLA
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., Li, W."Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells ". Oncology Letters 17.6 (2019): 4964-4968.
Chicago
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., Li, W."Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells ". Oncology Letters 17, no. 6 (2019): 4964-4968. https://doi.org/10.3892/ol.2019.10195
Copy and paste a formatted citation
x
Spandidos Publications style
Ye Y, Peng Y, Li Y, Liu C, Xu Y and Li W: Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells . Oncol Lett 17: 4964-4968, 2019.
APA
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., & Li, W. (2019). Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells . Oncology Letters, 17, 4964-4968. https://doi.org/10.3892/ol.2019.10195
MLA
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., Li, W."Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells ". Oncology Letters 17.6 (2019): 4964-4968.
Chicago
Ye, Y., Peng, Y., Li, Y., Liu, C., Xu, Y., Li, W."Effect of lincRNA-p21 targeting HIF-1α on biological functions of liver cancer cells ". Oncology Letters 17, no. 6 (2019): 4964-4968. https://doi.org/10.3892/ol.2019.10195
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