Design of a liver cancer‑specific selector for the analysis of circulating tumor DNA

Sun,Yan; Meng,Rui; Tang,Heng; Wang,Huimin; Guo,Xueqin; Ma,Yuanyuan; Yang,Yun; Wei,Xiaoming; Mu,Feng; Wu,Gang; Wang,Jun; Liu,Jun; Niu,Mingshan; Xue,Jun

doi:10.3892/ol.2019.10243

June-2019 Volume 17 Issue 6

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June-2019 Volume 17 Issue 6

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Article Open Access

Design of a liver cancer‑specific selector for the analysis of circulating tumor DNA

Authors:
- Yan Sun
- Rui Meng
- Heng Tang
- Huimin Wang
- Xueqin Guo
- Yuanyuan Ma
- Yun Yang
- Xiaoming Wei
- Feng Mu
- Gang Wu
- Jun Wang
- Jun Liu
- Mingshan Niu
- Jun Xue
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Affiliations: Department of Biology, University of Copenhagen, Copenhagen DK‑2200, Denmark, Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Hefei Anweikang Medical Laboratory, Hefei, Anhui 230000, P.R. China, Department of Neurology, Xinxiang Central Hospital, Xinxiang, Henan 453000, P.R. China, Wuhan Medical Laboratory, BGI‑Wuhan, Wuhan, Hubei 430075, P.R. China, BGI Genomics, BGI‑Shenzhen, Shenzhen, Guangdong 518083, P.R. China, Department of Bioscience and Bioengineering, South China University, Guangzhou, Guangdong 510641, P.R. China, Jiangsu Key Laboratory of Bone Marrow Stem Cell, Xuzhou Medical College, Xuzhou, Jiangsu 221002, P.R. China

Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
Pages: 5369-5376
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Published online on: April 12, 2019

https://doi.org/10.3892/ol.2019.10243
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Abstract

Circulating tumor DNA (ctDNA) has been frequently investigated to monitor tumor dynamics and measure tumor burden. This non‑invasive method concerning ctDNA has been recognized as a promising biomarker. Recently, next generation sequencing has been used in ctDNA detection by researchers. However, those reports have been limited by modest sensitivity, and only a minority of patients with cancer were applicable. Additionally, a limited number of cases of liver cancer have been analyzed. A more precise method is required to be established to evaluate ctDNA noninvasively. In the present study, a novel method to design a liver cancer‑associated chip region (spanning 211 kb, containing 159 genes) was performed with high specificity using International Cancer Genome Consortium datasets. Following evaluation with datasets from The Cancer Genome Atlas and data from 3 patients with liver cancer, the selected regions were demonstrated to be beneficial to locate specific somatic mutations associated with liver cancer therapy and to monitor cancer dynamics in the plasma samples of the patients. In addition to establishing performance benchmarks supporting direct clinical use, the chip designed and the high‑resolution sequencing analyses pipeline would allow the development a set of patient specific markers that could monitor the process of cancer with high accuracy and low cost. Furthermore, the present study is essential to understanding the dynamics and providing insight into the basic mechanisms of liver cancer.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Design of a liver cancer‑specific selector for the analysis of circulating tumor DNA

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