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Article Open Access

Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9

  • Authors:
    • Naohiro Tsuyama
    • Yu Abe
    • Aki Yanagi
    • Yukari Yanai
    • Misaki Sugai
    • Atsushi Katafuchi
    • Fumihiko Kawamura
    • Kenji Kamiya
    • Akira Sakai
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Life Sciences, Fukushima Medical University, Fukushima 960‑1295, Japan, Department of Experimental Oncology, Research Institute for Radiation Biology and Medicine, Hiroshima University, Minami‑ku, Hiroshima 734‑8553, Japan
    Copyright: © Tsuyama et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 275-282
    |
    Published online on: May 2, 2019
       https://doi.org/10.3892/ol.2019.10303
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Abstract

Chromosomal translocation is a key process in the oncogenic transformation of somatic cells. Previously, artificial induction of chromosomal translocation was performed using homologous recombination‑mediated loxP labeling of target regions followed by Cre‑mediated recombination. Recent progress in genome editing techniques has facilitated the easier induction of artificial translocation by cutting two targeted genome sequences from different chromosomes. The present study established a system to induce t(11;14)(q13;q32), which is observed primarily in multiple myeloma (MM) and involves the repositioning of the cyclin D1 (CCND1) gene downstream of the immunoglobulin heavy chain (IgH) constant region enhancers by translocation. The placing of tandem gRNAs designed to cut both the IgH Eµ and CCND1 15‑kb upstream regions in lentiCRISPRv2 enabled the induction of chromosomal translocation in 293T cells, with confirmation by translocation‑specific PCR and fluorescence in situ hybridization probing with IgH and CCND1. At the translocation junctions, small deletions and the addition of DNA sequences (indels) were observed in several clones. Cloned cells with t(11;14) exhibited slower growth and lower CCND1 mRNA expression compared to the parent cells, presenting the opposite phenomena induced by t(11;14) in MM cells, indicating that the silent IgH gene juxtaposed to CCND1 may negatively affect CCND1 gene expression and cell proliferation in the non‑B lymphocyte lineage. Therefore, the present study achieved the induction of silent promoter/enhancer translocation in t(11;14)(q13;q32) as a preparatory experiment to study the role of IgH constant region enhancer‑driven CCND1 overexpression in oncogenic transformation processes in B lymphocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Tsuyama N, Abe Y, Yanagi A, Yanai Y, Sugai M, Katafuchi A, Kawamura F, Kamiya K and Sakai A: Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9. Oncol Lett 18: 275-282, 2019.
APA
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A. ... Sakai, A. (2019). Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9. Oncology Letters, 18, 275-282. https://doi.org/10.3892/ol.2019.10303
MLA
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A., Kawamura, F., Kamiya, K., Sakai, A."Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9". Oncology Letters 18.1 (2019): 275-282.
Chicago
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A., Kawamura, F., Kamiya, K., Sakai, A."Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9". Oncology Letters 18, no. 1 (2019): 275-282. https://doi.org/10.3892/ol.2019.10303
Copy and paste a formatted citation
x
Spandidos Publications style
Tsuyama N, Abe Y, Yanagi A, Yanai Y, Sugai M, Katafuchi A, Kawamura F, Kamiya K and Sakai A: Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9. Oncol Lett 18: 275-282, 2019.
APA
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A. ... Sakai, A. (2019). Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9. Oncology Letters, 18, 275-282. https://doi.org/10.3892/ol.2019.10303
MLA
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A., Kawamura, F., Kamiya, K., Sakai, A."Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9". Oncology Letters 18.1 (2019): 275-282.
Chicago
Tsuyama, N., Abe, Y., Yanagi, A., Yanai, Y., Sugai, M., Katafuchi, A., Kawamura, F., Kamiya, K., Sakai, A."Induction of t(11;14) IgH enhancer/promoter‑cyclin D1 gene translocation using CRISPR/Cas9". Oncology Letters 18, no. 1 (2019): 275-282. https://doi.org/10.3892/ol.2019.10303
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