Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-2019 Volume 18 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-2019 Volume 18 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines

  • Authors:
    • Wiebke Siekmann
    • Elisabet Tina
    • Anita Koskela von Sydow
    • Anil Gupta
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology and Intensive Care, University Hospital Örebro and School of Medical Sciences, Örebro University, SE‑70185 Örebro, Sweden, Department of Clinical Research Laboratory, Faculty of Medicine and Health, Örebro University, SE‑70185 Örebro, Sweden, Department of Physiology and Pharmacology, Karolinska Institute and Karolinska University Hospital, SE‑17176 Stockholm, Sweden
  • Pages: 395-401
    |
    Published online on: May 7, 2019
       https://doi.org/10.3892/ol.2019.10332
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Regional anesthesia may prolong survival following surgery for different types of cancers. The mechanisms behind this are unclear but direct effects on cancer cells by local anesthetics (LA) have been suggested. The aim of this study was to investigate if lidocaine or ropivacaine have a dose‑dependent effect on the cell viability and proliferation of a primary and a secondary colon carcinoma cell line in vitro. The colon cancer cell lines SW480 derived from primary tumor and SW620 from a metastatic site in the same patient were exposed to increasing concentrations of lidocaine and ropivacaine (5‑1,000 µM). Cell viability was measured using CellTiter‑Blue® and cell proliferation by PKH67 after exposure for up to 72 h. Cell viability was significantly reduced by ropivacaine at the highest concentration (1,000 µM) after 48 and 72 h in the cell line SW480 and at 72 h in SW620. Exposure to lidocaine did not show any significant reduction in cell viability. Notably, low concentrations of both lidocaine and ropivacaine significantly increased cell viability after 48 and 72 h in SW620. Cell proliferation was significantly reduced by 1,000 µM lidocaine in SW480 and by 1,000 µM ropivacaine in SW620. In summary, both lidocaine and ropivacaine showed an anti‑proliferative effect in the colon cancer cell lines at high concentrations and after prolonged exposure to LA in vitro. Our findings also indicate that lower concentrations promote cell viability in the metastatic cell line.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

View References

1 

Ceelen W, Pattyn P and Mareel M: Surgery, wound healing, and metastasis: Recent insights and clinical implications. Crit Rev Oncol Hematol. 89:16–26. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Coffey JC, Wang JH, Smith MJ, Bouchier-Hayes D, Cotter TG and Redmond HP: Excisional surgery for cancer cure: therapy at a cost. Lancet Oncol. 4:760–768. 2003. View Article : Google Scholar : PubMed/NCBI

3 

Gupta A, Björnsson A, Fredriksson M, Hallböök O and Eintrei C: Reduction in mortality after epidural anaesthesia and analgesia in patients undergoing rectal but not colonic cancer surgery: a retrospective analysis of data from 655 patients in central Sweden. Br J Anaesth. 107:164–170. 2011. View Article : Google Scholar : PubMed/NCBI

4 

Christopherson R, James KE, Tableman M, Marshall P and Johnson FE: Long-term survival after colon cancer surgery: a variation associated with choice of anesthesia. Anesth Analg. 107:325–332. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Biki B, Mascha E, Moriarty DC, Fitzpatrick JM, Sessler DI and Buggy DJ: Anesthetic technique for radical prostatectomy surgery affects cancer recurrence: a retrospective analysis. Anesthesiology. 109:180–187. 2008. View Article : Google Scholar : PubMed/NCBI

6 

Beilin B, Shavit Y, Trabekin E, Mordashev B, Mayburd E, Zeidel A and Bessler H: The effects of postoperative pain management on immune response to surgery. Anesth Analg. 97:822–827. 2003. View Article : Google Scholar : PubMed/NCBI

7 

Kurosawa S: Anesthesia in patients with cancer disorders. Curr Opin Anaesthesiol. 25:376–384. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Chang YC, Liu CL, Chen MJ, Hsu YW, Chen SN, Lin CH, Chen CM, Yang FM and Hu MC: Local anesthetics induce apoptosis in human breast tumor cells. Anesth Analg. 118:116–124. 2014. View Article : Google Scholar : PubMed/NCBI

9 

Lirk P, Berger R, Hollmann MW and Fiegl H: Lidocaine time- and dose-dependently demethylates deoxyribonucleic acid in breast cancer cell lines in vitro. Br J Anaesth. 109:200–207. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Baptista-Hon DT, Robertson FM, Robertson GB, Owen SJ, Rogers GW, Lydon EL, Lee NH and Hales TG: Potent inhibition by ropivacaine of metastatic colon cancer SW620 cell invasion and NaV1.5 channel function. Br J Anaesth. 113 (Suppl 1):i39–i48. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Perez-Castro R, Patel S, Garavito-Aguilar ZV, Rosenberg A, Recio-Pinto E, Zhang J, Blanck TJ and Xu F: Cytotoxicity of local anesthetics in human neuronal cells. Anesth Analg. 108:997–1007. 2009. View Article : Google Scholar : PubMed/NCBI

12 

Lucchinetti E, Awad AE, Rahman M, Feng J, Lou PH, Zhang L, Ionescu L, Lemieux H, Thébaud B and Zaugg M: Antiproliferative effects of local anesthetics on mesenchymal stem cells: potential implications for tumor spreading and wound healing. Anesthesiology. 116:841–856. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Alizadeh AM, Shiri S and Farsinejad S: Metastasis review: from bench to bedside. Tumour Biol. 35:8483–8523. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wiedemann D, Mühlnickel B, Staroske E, Neumann W and Röse W: Ropivacaine plasma concentrations during 120-hour epidural infusion. Br J Anaesth. 85:830–835. 2000. View Article : Google Scholar : PubMed/NCBI

15 

Mayumi T, Dohi S and Takahashi T: Plasma concentrations of lidocaine associated with cervical, thoracic, and lumbar epidural anesthesia. Anesth Analg. 62:578–580. 1983. View Article : Google Scholar : PubMed/NCBI

16 

Hollmann MW and Durieux ME: Local anesthetics and the inflammatory response: a new therapeutic indication? Anesthesiology. 93:858–875. 2000. View Article : Google Scholar : PubMed/NCBI

17 

Leibovitz A, Stinson JC, McCombs WB III, McCoy CE, Mazur KC and Mabry ND: Classification of human colorectal adenocarcinoma cell lines. Cancer Res. 36:4562–4569. 1976.PubMed/NCBI

18 

Rampersad SN: Multiple applications of Alamar Blue as an indicator of metabolic function and cellular health in cell viability bioassays. Sensors (Basel). 12:12347–12360. 2012. View Article : Google Scholar : PubMed/NCBI

19 

Xuan W, Zhao H, Hankin J, Chen L, Yao S and Ma D: Local anesthetic bupivacaine induced ovarian and prostate cancer apoptotic cell death and underlying mechanisms in vitro. Sci Rep. 6:262772016. View Article : Google Scholar : PubMed/NCBI

20 

Le Gac G, Angenard G, Clément B, Laviolle B, Coulouarn C and Beloeil H: Local anesthetics inhibit the growth of human hepatocellular carcinoma cells. Anesth Analg. 125:1600–1609. 2017. View Article : Google Scholar : PubMed/NCBI

21 

Vogelaar FJ, Abegg R, van der Linden JC, Cornelisse HG, van Dorsten FR, Lemmens VE and Bosscha K: Epidural analgesia associated with better survival in colon cancer. Int J Colorectal Dis. 30:1103–1107. 2015. View Article : Google Scholar : PubMed/NCBI

22 

Votta-Velis EG, Piegeler T, Minshall RD, Aguirre J, Beck-Schimmer B, Schwartz DE and Borgeat A: Regional anaesthesia and cancer metastases: the implication of local anaesthetics. Acta Anaesthesiol Scand. 57:1211–1229. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Hanahan D and Weinberg RA: Hallmarks of cancer: the next generation. Cell. 144:646–674. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Piegeler T, Votta-Velis EG, Liu G, Place AT, Schwartz DE, Beck-Schimmer B, Minshall RD and Borgeat A: Antimetastatic potential of amide-linked local anesthetics: inhibition of lung adenocarcinoma cell migration and inflammatory Src signaling independent of sodium channel blockade. Anesthesiology. 117:548–559. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Piegeler T, Schläpfer M, Dull RO, Schwartz DE, Borgeat A, Minshall RD and Beck-Schimmer B: Clinically relevant concentrations of lidocaine and ropivacaine inhibit TNFα-induced invasion of lung adenocarcinoma cells in vitro by blocking the activation of Akt and focal adhesion kinase. Br J Anaesth. 115:784–791. 2015. View Article : Google Scholar : PubMed/NCBI

26 

Brackenbury WJ: Voltage-gated sodium channels and metastatic disease. Channels (Austin). 6:352–361. 2012. View Article : Google Scholar : PubMed/NCBI

27 

House CD, Vaske CJ, Schwartz AM, Obias V, Frank B, Luu T, Sarvazyan N, Irby R, Strausberg RL, Hales TG, et al: Voltage-gated Na+ channel SCN5A is a key regulator of a gene transcriptional network that controls colon cancer invasion. Cancer Res. 70:6957–6967. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Martinsson T: Ropivacaine inhibits serum-induced proliferation of colon adenocarcinoma cells in vitro. J Pharmacol Exp Ther. 288:660–664. 1999.PubMed/NCBI

29 

Biazik JM, Jahn KA, Su Y, Wu YN and Braet F: Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining. World J Gastroenterol. 16:2743–2753. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Lambert AW, Pattabiraman DR and Weinberg RA: Emerging biological principles of metastasis. Cell. 168:670–691. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Tohme S, Simmons RL and Tsung A: Surgery for Cancer: a trigger for metastases. Cancer Res. 77:1548–1552. 2017. View Article : Google Scholar : PubMed/NCBI

32 

Talmadge JE and Fidler IJ: AACR centennial series: the biology of cancer metastasis: historical perspective. Cancer Res. 70:5649–5669. 2010. View Article : Google Scholar : PubMed/NCBI

33 

Hewitt RE, McMarlin A, Kleiner D, Wersto R, Martin P, Tsokos M, Stamp GW and Stetler-Stevenson WG: Validation of a model of colon cancer progression. J Pathol. 192:446–454. 2000. View Article : Google Scholar : PubMed/NCBI

34 

Bundscherer A, Malsy M, Gebhardt K, Metterlein T, Plank C, Wiese CH, Gruber M and Graf BM: Effects of ropivacaine, bupivacaine and sufentanil in colon and pancreatic cancer cells in vitro. Pharmacol Res 95–96. 126–131. 2015. View Article : Google Scholar

35 

Sztark F, Malgat M, Dabadie P and Mazat JP: Comparison of the effects of bupivacaine and ropivacaine on heart cell mitochondrial bioenergetics. Anesthesiology. 88:1340–1349. 1998. View Article : Google Scholar : PubMed/NCBI

36 

Nouette-Gaulain K, Sirvent P, Canal-Raffin M, Morau D, Malgat M, Molimard M, Mercier J, Lacampagne A, Sztark F and Capdevila X: Effects of intermittent femoral nerve injections of bupivacaine, levobupivacaine, and ropivacaine on mitochondrial energy metabolism and intracellular calcium homeostasis in rat psoas muscle. Anesthesiology. 106:1026–1034. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Siekmann W, Tina E, Koskela von Sydow A and Gupta A: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines. Oncol Lett 18: 395-401, 2019.
APA
Siekmann, W., Tina, E., Koskela von Sydow, A., & Gupta, A. (2019). Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines. Oncology Letters, 18, 395-401. https://doi.org/10.3892/ol.2019.10332
MLA
Siekmann, W., Tina, E., Koskela von Sydow, A., Gupta, A."Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines". Oncology Letters 18.1 (2019): 395-401.
Chicago
Siekmann, W., Tina, E., Koskela von Sydow, A., Gupta, A."Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines". Oncology Letters 18, no. 1 (2019): 395-401. https://doi.org/10.3892/ol.2019.10332
Copy and paste a formatted citation
x
Spandidos Publications style
Siekmann W, Tina E, Koskela von Sydow A and Gupta A: Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines. Oncol Lett 18: 395-401, 2019.
APA
Siekmann, W., Tina, E., Koskela von Sydow, A., & Gupta, A. (2019). Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines. Oncology Letters, 18, 395-401. https://doi.org/10.3892/ol.2019.10332
MLA
Siekmann, W., Tina, E., Koskela von Sydow, A., Gupta, A."Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines". Oncology Letters 18.1 (2019): 395-401.
Chicago
Siekmann, W., Tina, E., Koskela von Sydow, A., Gupta, A."Effect of lidocaine and ropivacaine on primary (SW480) and metastatic (SW620) colon cancer cell lines". Oncology Letters 18, no. 1 (2019): 395-401. https://doi.org/10.3892/ol.2019.10332
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team