Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer

Peng,Lijun; Li,Shaobin; Li,Yuchan; Wan,Minghui; Fang,Xisheng; Zhao,Yongxin; Zuo,Wei; Long,De; Xuan,Yiwen

doi:10.3892/ol.2019.10333

Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer

Authors:
- Lijun Peng
- Shaobin Li
- Yuchan Li
- Minghui Wan
- Xisheng Fang
- Yongxin Zhao
- Wei Zuo
- De Long
- Yiwen Xuan
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Affiliations: Department of Thoracic Surgery, Guangzhou General Hospital of The People's Liberation Army, Guangzhou, Guangdong 510010, P.R. China, Department of Cardiothoracic Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Oncology Department 2, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Radiation Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China, Department of Medical Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Oncology, The Cooperation of Chinese and Western Medicine Hospital in Guangzhou, Guangzhou, Guangdong 510800, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 33000, P.R. China
Published online on: May 7, 2019 https://doi.org/10.3892/ol.2019.10333
Pages: 137-144

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Abstract

The present study aimed to evaluate microRNA‑20b‑5p (miR‑20b‑5p) expression in non‑small cell lung cancer (NSCLC), and investigate the effects of miR‑20b‑5p expression on NSCLC cell proliferation and migration. Reverse transcription‑quantitative polymerase chain reaction was performed to measure the expression level of miR‑20b‑5p in NSCLC tissues and cell lines. Cell Counting Kit‑8 and wound healing assays were used to measure cell proliferation and migration. A dual‑luciferase reporter assay was performed to validate B‑cell translocation gene 3 (BTG3) as a target of miR‑20b‑5p. It was identified that the expression level of miR‑20b‑5p is elevated in NSCLC tissues and cell lines. miR‑20b‑5p overexpression was revealed to promote NSCLC cell proliferation and migration. Furthermore, BTG3 was identified as a direct target of miR‑20b‑5p, and BTG3 overexpression reversed a miR‑20b‑5p mimic‑induced increase in cell proliferation and migration. In summary, the present study revealed that miR‑20b‑5p promotes NSCLC cell proliferation and migration by targeting BTG3, which may assist with the development of a novel therapeutic target for the treatment of NSCLC.

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