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Article

Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer

  • Authors:
    • Lijun Peng
    • Shaobin Li
    • Yuchan Li
    • Minghui Wan
    • Xisheng Fang
    • Yongxin Zhao
    • Wei Zuo
    • De Long
    • Yiwen Xuan
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Guangzhou General Hospital of The People's Liberation Army, Guangzhou, Guangdong 510010, P.R. China, Department of Cardiothoracic Surgery, Zhujiang Hospital of Southern Medical University, Guangzhou, Guangdong 510280, P.R. China, Oncology Department 2, Guangdong Second Provincial General Hospital, Guangzhou, Guangdong 510317, P.R. China, Department of Radiation Oncology, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, Guangdong 510260, P.R. China, Department of Medical Oncology, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Oncology, The Cooperation of Chinese and Western Medicine Hospital in Guangzhou, Guangzhou, Guangdong 510800, P.R. China, Department of Respiratory Medicine, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 33000, P.R. China
  • Pages: 137-144
    |
    Published online on: May 7, 2019
       https://doi.org/10.3892/ol.2019.10333
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Abstract

The present study aimed to evaluate microRNA‑​20b‑5p (miR‑20b‑5p) expression in non‑small cell lung cancer (NSCLC), and investigate the effects of miR‑20b‑5p expression on NSCLC cell proliferation and migration. Reverse transcription‑quantitative polymerase chain reaction was performed to measure the expression level of miR‑20b‑5p in NSCLC tissues and cell lines. Cell Counting Kit‑8 and wound healing assays were used to measure cell proliferation and migration. A dual‑luciferase reporter assay was performed to validate B‑cell translocation gene 3 (BTG3) as a target of miR‑20b‑5p. It was identified that the expression level of miR‑20b‑5p is elevated in NSCLC tissues and cell lines. miR‑20b‑5p overexpression was revealed to promote NSCLC cell proliferation and migration. Furthermore, BTG3 was identified as a direct target of miR‑20b‑5p, and BTG3 overexpression reversed a miR‑20b‑5p mimic‑induced increase in cell proliferation and migration. In summary, the present study revealed that miR‑20b‑5p promotes NSCLC cell proliferation and migration by targeting BTG3, which may assist with the development of a novel therapeutic target for the treatment of NSCLC.
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1 

Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J and Jemal A: Global cancer statistics, 2012. CA Cancer J Clin. 65:87–108. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Gompelmann D, Eberhardt R and Herth FJ: Advanced malignant lung disease: What the specialist can offer. Respiration. 82:111–123. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Kang M, Shi J, Peng N and He S: MicroRNA-211 promotes non-small-cell lung cancer proliferation and invasion by targeting MxA. Onco Targets Ther. 10:5667–5675. 2017. View Article : Google Scholar : PubMed/NCBI

4 

Siegel RL, Miller KD and Jemal A: Cancer statistics, 2017. CA Cancer J Clin. 67:7–30. 2017. View Article : Google Scholar : PubMed/NCBI

5 

Mallory AC and Vaucheret H: MicroRNAs: Something important between the genes. Curr Opin Plant Biol. 7:120–125. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Hwang HW and Mendell JT: MicroRNAs in cell proliferation, cell death, and tumorigenesis. Br J Cancer. 94:776–780. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Florczuk M, Szpechcinski A and Chorostowska-Wynimko J: miRNAs as biomarkers and therapeutic targets in non-small cell lung cancer: Current perspectives. Target Oncol. 12:179–200. 2017. View Article : Google Scholar : PubMed/NCBI

10 

Zhang B, Pan X, Cobb GP and Anderson TA: microRNAs as oncogenes and tumor suppressors. Dev Biol. 302:1–12. 2007. View Article : Google Scholar : PubMed/NCBI

11 

Saleiban A, Faxälv L, Claesson K, Jönsson JI and Osman A: miR-20b regulates expression of proteinase-activated receptor-1 (PAR-1) thrombin receptor in melanoma cells. Pigment Cell Melanoma Res. 27:431–441. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Li Y, Chen D, Jin L, Liu J, Su Z, Li Y, Gui Y and Lai Y: MicroRNA-20b-5p functions as a tumor suppressor in renal cell carcinoma by regulating cellular proliferation, migration and apoptosis. Mol Med Rep. 13:1895–1901. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Li M, Zhou Y, Xia T, Zhou X, Huang Z, Zhang H, Zhu W, Ding Q and Wang S: Circulating microRNAs from the miR-106a-363 cluster on chromosome X as novel diagnostic biomarkers for breast cancer. Breast Cancer Res Treat. 170:257–270. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Leidinger P, Brefort T, Backes C, Krapp M, Galata V, Beier M, Kohlhaas J, Huwer H, Meese E and Keller A: High-throughput qRT-PCR validation of blood microRNAs in non-small cell lung cancer. Oncotarget. 7:4611–4623. 2016. View Article : Google Scholar : PubMed/NCBI

15 

Greene FL, Page DL, Fleming ID, Fritz A, Balch CM and Haller DG: AJCC cancer staging handbook from the AJCC cancer staging manual. (6th). Springer. (New York). 2002.

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Gao X, Zhao H, Diao C, Wang X, Xie Y, Liu Y, Han J and Zhang M: miR-455-3p serves as prognostic factor and regulates the proliferation and migration of non-small cell lung cancer through targeting HOXB5. Biochem Biophys Res Commun. 495:1074–1080. 2018. View Article : Google Scholar : PubMed/NCBI

18 

Tanzer A and Stadler PF: Molecular evolution of a microRNA cluster. J Mol Biol. 339:327–335. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Hayashita Y, Osada H, Tatematsu Y, Yamada H, Yanagisawa K, Tomida S, Yatabe Y, Kawahara K, Sekido Y and Takahashi T: A polycistronic microRNA cluster, miR-17-92, is overexpressed in human lung cancers and enhances cell proliferation. Cancer Res. 65:9628–9632. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Landais S, Landry S, Legault P and Rassart E: Oncogenic potential of the miR-106-363 cluster and its implication in human T-cell leukemia. Cancer Res. 67:5699–5707. 2007. View Article : Google Scholar : PubMed/NCBI

21 

Cheng YC, Lin TY and Shieh SY: Candidate tumor suppressor BTG3 maintains genomic stability by promoting Lys63-linked ubiquitination and activation of the checkpoint kinase CHK1. Proc Natl Acad Sci USA. 110:5993–5998. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Majid S, Dar AA, Ahmad AE, Hirata H, Kawakami K, Shahryari V, Saini S, Tanaka Y, Dahiya AV, Khatri G and Dahiya R: BTG3 tumor suppressor gene promoter demethylation, histone modification and cell cycle arrest by genistein in renal cancer. Carcinogenesis. 30:662–670. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Lv C, Wang H, Tong Y, Yin H, Wang D, Yan Z, Liang Y, Wu D and Su Q: The function of BTG3 in colorectal cancer cells and its possible signaling pathway. J Cancer Res Clin Oncol. 144:295–308. 2018. View Article : Google Scholar : PubMed/NCBI

24 

An Q, Zhou Y, Han C, Zhou Y, Li F and Li D: BTG3 overexpression suppresses the proliferation and invasion in epithelial ovarian cancer cell by regulating AKT/GSK3β/β-catenin signaling. Reprod Sci. 24:1462–1468. 2017. View Article : Google Scholar : PubMed/NCBI

25 

Chen X, Chen G, Cao X, Zhou Y, Yang T and Wei S: Downregulation of BTG3 in non-small cell lung cancer. Biochem Biophys Res Commun. 437:173–178. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Guo J, Xiao ZW, Yu XW and Cao RF: miR-20b promotes cellular proliferation and migration by directly regulating phosphatase and tensin homolog in prostate cancer. Oncol Lett. 14:6895–6900. 2017.PubMed/NCBI

27 

Luo W, Li G, Yi Z, Nie Q and Zhang X: E2F1-miR-20a-5p/20b-5p auto-regulatory feedback loop involved in myoblast proliferation and differentiation. Sci Rep. 6:279042016. View Article : Google Scholar : PubMed/NCBI

28 

Ou YH, Chung PH, Hsu FF, Sun TP, Chang WY and Shieh SY: The candidate tumor suppressor BTG3 is a transcriptional target of p53 that inhibits E2F1. EMBO J. 26:3968–3980. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Peng L, Li S, Li Y, Wan M, Fang X, Zhao Y, Zuo W, Long D and Xuan Y: Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer. Oncol Lett 18: 137-144, 2019.
APA
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y. ... Xuan, Y. (2019). Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer. Oncology Letters, 18, 137-144. https://doi.org/10.3892/ol.2019.10333
MLA
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y., Zuo, W., Long, D., Xuan, Y."Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer". Oncology Letters 18.1 (2019): 137-144.
Chicago
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y., Zuo, W., Long, D., Xuan, Y."Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer". Oncology Letters 18, no. 1 (2019): 137-144. https://doi.org/10.3892/ol.2019.10333
Copy and paste a formatted citation
x
Spandidos Publications style
Peng L, Li S, Li Y, Wan M, Fang X, Zhao Y, Zuo W, Long D and Xuan Y: Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer. Oncol Lett 18: 137-144, 2019.
APA
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y. ... Xuan, Y. (2019). Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer. Oncology Letters, 18, 137-144. https://doi.org/10.3892/ol.2019.10333
MLA
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y., Zuo, W., Long, D., Xuan, Y."Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer". Oncology Letters 18.1 (2019): 137-144.
Chicago
Peng, L., Li, S., Li, Y., Wan, M., Fang, X., Zhao, Y., Zuo, W., Long, D., Xuan, Y."Regulation of BTG3 by microRNA‑20b‑5p in non‑small cell lung cancer". Oncology Letters 18, no. 1 (2019): 137-144. https://doi.org/10.3892/ol.2019.10333
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