Open Access

Genome‑wide ChIP‑seq data with a transcriptome analysis reveals the groups of genes regulated by histone demethylase LSD1 inhibition in esophageal squamous cell carcinoma cells

  • Authors:
    • Isamu Hoshino
    • Masahiko Takahashi
    • Yasunori Akutsu
    • Kentaro Murakami
    • Yasunori Matsumoto
    • Hiroshi Suito
    • Nobufumi Sekino
    • Aki Komatsu
    • Keiko Iida
    • Takayoshi Suzuki
    • Itsuro Inoue
    • Fumitaka Ishige
    • Yosuke Iwatate
    • Hisahiro Matsubara
  • View Affiliations

  • Published online on: May 13, 2019     https://doi.org/10.3892/ol.2019.10350
  • Pages: 872-881
  • Copyright: © Hoshino et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Expression of genes is controlled by histone modification, histone acetylation and methylation, but abnormalities of these modifications have been observed in carcinogenesis and cancer development. The effect of the lysine‑specific histone demethylase 1 (LSD1) inhibitor, a demethylating enzyme of histones, is thought to be caused by controlling the expression of genes. The aim of the present study is to elucidate the efficacies of the LSD1 inhibitor on the gene expression of esophageal cancer cell lines using chromatin immunoprecipitation (ChIP)‑Seq. A comprehensive analysis of gene expression changes in esophageal squamous cell carcinoma (ESCC) cell lines induced by the LSD1 inhibitor NCL1 was clarified via analysis using microarray. In addition, ChIP‑seq analysis was conducted using a SimpleChIP plus Enzymatic Chromatin IP kit. NCL1 strongly suppressed the proliferation of T.Tn and TE2 cells, which are ESCC cell lines, and further induced apoptosis. According to the combinatory analysis of ChIP‑seq and microarray, 17 genes were upregulated, and 16 genes were downregulated in both cell lines. The comprehensive gene expression study performed in the present study is considered to be useful for analyzing the mechanism of the antitumor effect of the LSD1 inhibitor in patients with ESCC.
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July-2019
Volume 18 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Hoshino I, Takahashi M, Akutsu Y, Murakami K, Matsumoto Y, Suito H, Sekino N, Komatsu A, Iida K, Suzuki T, Suzuki T, et al: Genome‑wide ChIP‑seq data with a transcriptome analysis reveals the groups of genes regulated by histone demethylase LSD1 inhibition in esophageal squamous cell carcinoma cells. Oncol Lett 18: 872-881, 2019.
APA
Hoshino, I., Takahashi, M., Akutsu, Y., Murakami, K., Matsumoto, Y., Suito, H. ... Matsubara, H. (2019). Genome‑wide ChIP‑seq data with a transcriptome analysis reveals the groups of genes regulated by histone demethylase LSD1 inhibition in esophageal squamous cell carcinoma cells. Oncology Letters, 18, 872-881. https://doi.org/10.3892/ol.2019.10350
MLA
Hoshino, I., Takahashi, M., Akutsu, Y., Murakami, K., Matsumoto, Y., Suito, H., Sekino, N., Komatsu, A., Iida, K., Suzuki, T., Inoue, I., Ishige, F., Iwatate, Y., Matsubara, H."Genome‑wide ChIP‑seq data with a transcriptome analysis reveals the groups of genes regulated by histone demethylase LSD1 inhibition in esophageal squamous cell carcinoma cells". Oncology Letters 18.1 (2019): 872-881.
Chicago
Hoshino, I., Takahashi, M., Akutsu, Y., Murakami, K., Matsumoto, Y., Suito, H., Sekino, N., Komatsu, A., Iida, K., Suzuki, T., Inoue, I., Ishige, F., Iwatate, Y., Matsubara, H."Genome‑wide ChIP‑seq data with a transcriptome analysis reveals the groups of genes regulated by histone demethylase LSD1 inhibition in esophageal squamous cell carcinoma cells". Oncology Letters 18, no. 1 (2019): 872-881. https://doi.org/10.3892/ol.2019.10350