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MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer

  • Authors:
    • Chen Wei
    • Liu Lei
    • Huang Hui
    • Zhang Tao
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Molecular Diagnosis of Hubei Province, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China, Department of Gastrointestinal Surgery, Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430014, P.R. China
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 856-863
    |
    Published online on: May 14, 2019
       https://doi.org/10.3892/ol.2019.10358
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Abstract

An increasing number of studies have confirmed that miR‑124 exhibits a suppressive role in glioblastoma, cervical cancer and breast cancer; however, the function of miR‑124 in colorectal cancer (CRC) has not been completely elucidated. In the present study, miR‑124 expression was confirmed by reverse transcription‑quantitative PCR in 80 colorectal tissues and para‑cancerous tissues. The influence of altered miR‑124 expression was analyzed by statistical approaches including Cox multivariate regression analysis and the Kaplan‑Meier method, and the target genes of miR‑124 were confirmed by luciferase reporter assays. Immunohistochemical techniques were also performed in order to measure the expression levels of target proteins. miR‑124 expression was observed to be decreased in colorectal tissue samples, and this phenomenon was correlated with adverse clinical indicators and poor patient survival time. Luciferase reporter assays indicated that miR‑124 directly regulated TNF receptor associated factor 6 (TRAF6) 3'‑untranslated region (UTR). Hence, it was proposed that miR‑124 dysregulation may negatively influence the expression of TRAF6 and therefore serve as a biomarker of epithelial‑mesenchymal transition in CRC tissues. In summary, the present study demonstrated that miR‑124 regulates the expression of TRAF6, and may potentially function as an independent prognostic factor and therapeutic target in patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Wei C, Lei L, Hui H and Tao Z: MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer. Oncol Lett 18: 856-863, 2019.
APA
Wei, C., Lei, L., Hui, H., & Tao, Z. (2019). MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer. Oncology Letters, 18, 856-863. https://doi.org/10.3892/ol.2019.10358
MLA
Wei, C., Lei, L., Hui, H., Tao, Z."MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer". Oncology Letters 18.1 (2019): 856-863.
Chicago
Wei, C., Lei, L., Hui, H., Tao, Z."MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer". Oncology Letters 18, no. 1 (2019): 856-863. https://doi.org/10.3892/ol.2019.10358
Copy and paste a formatted citation
x
Spandidos Publications style
Wei C, Lei L, Hui H and Tao Z: MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer. Oncol Lett 18: 856-863, 2019.
APA
Wei, C., Lei, L., Hui, H., & Tao, Z. (2019). MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer. Oncology Letters, 18, 856-863. https://doi.org/10.3892/ol.2019.10358
MLA
Wei, C., Lei, L., Hui, H., Tao, Z."MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer". Oncology Letters 18.1 (2019): 856-863.
Chicago
Wei, C., Lei, L., Hui, H., Tao, Z."MicroRNA‑124 regulates TRAF6 expression and functions as an independent prognostic factor in colorectal cancer". Oncology Letters 18, no. 1 (2019): 856-863. https://doi.org/10.3892/ol.2019.10358
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