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Article Open Access

Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma

  • Authors:
    • Yuanyuan Tang
    • Xiuzhen Sun
    • Shen Yu
    • Xu Bie
    • Jizhe Wang
    • Lidan Ren
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, P.R. China, State Key Laboratory of Structural Analysis for Industrial Equipment, Dalian University of Technology, Dalian, Liaoning 116023, P.R. China, Department of Oncology, The 210th Hospital of PLA, Dalian, Liaoning 116000, P.R. China
    Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 553-560
    |
    Published online on: May 17, 2019
       https://doi.org/10.3892/ol.2019.10370
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Abstract

Nasopharyngeal carcinoma (NPC) is one of the most severe types of malignant cancer of the head and neck as it is difficult to treat. Ezrin is highly expressed in numerous types of cancer. However, the role of Ezrin in NPC has not been fully investigated and further studies are required in order to uncover its therapeutic potential in the treatment of NPC. The aim of the present study was to investigate the expression of Ezrin in human NPC and to evaluate the effect of knockdown of Ezrin using small interfering (si)‑RNA on NPC cell migration and invasion. The expression levels of Ezrin were determined using reverse transcription‑quantitative polymerase chain reaction, immunohistochemical staining and western blotting. Following transfection of Ezrin‑siRNA into NPC cells, cell invasion and migration were analyzed and the mRNA expression levels of matrix metalloproteinase(MMP)‑2 and MMP9 were determined. The results revealed that the expression of Ezrin was markedly increased in human NPC tissue samples compared with normal adjacent nasopharyngeal tissue samples. Ezrin was also highly expressed in the NPC cell lines 6‑10B and C6661 when compared with the normal nasopharyngeal cell line NP69. Transfection of NPC cell lines with siRNA targeting Ezrin significantly inhibited NPC cell migration and invasion, and downregulated the mRNA expression level of MMP2; however, no effect was observed on MMP9 mRNA expression. At the same time, knockdown of Ezrin significantly decreased the expression levels of phosphatidylinositol 3‑kinase (PI3K) and phosphorylated protein kinase B (Akt), which downregulated the mRNA expression of MMP2. In conclusion, the results revealed that knockdown of Ezrin suppressed NPC migration and invasion by reducing the mRNA expression of MMP2 via the PI3K/Akt signaling pathway. These results highlight the important role of Ezrin in NPC cell migration and invasion. In addition, they indicate that silencing of Ezrin may serve as a potential therapeutic strategy to treat human NPC.
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Copy and paste a formatted citation
Spandidos Publications style
Tang Y, Sun X, Yu S, Bie X, Wang J and Ren L: Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncol Lett 18: 553-560, 2019.
APA
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., & Ren, L. (2019). Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncology Letters, 18, 553-560. https://doi.org/10.3892/ol.2019.10370
MLA
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., Ren, L."Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma". Oncology Letters 18.1 (2019): 553-560.
Chicago
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., Ren, L."Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma". Oncology Letters 18, no. 1 (2019): 553-560. https://doi.org/10.3892/ol.2019.10370
Copy and paste a formatted citation
x
Spandidos Publications style
Tang Y, Sun X, Yu S, Bie X, Wang J and Ren L: Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncol Lett 18: 553-560, 2019.
APA
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., & Ren, L. (2019). Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma. Oncology Letters, 18, 553-560. https://doi.org/10.3892/ol.2019.10370
MLA
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., Ren, L."Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma". Oncology Letters 18.1 (2019): 553-560.
Chicago
Tang, Y., Sun, X., Yu, S., Bie, X., Wang, J., Ren, L."Inhibition of Ezrin suppresses cell migration and invasion in human nasopharyngeal carcinoma". Oncology Letters 18, no. 1 (2019): 553-560. https://doi.org/10.3892/ol.2019.10370
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