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Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer

  • Authors:
    • Xiaoming Tan
    • Sufen Zhang
    • Huifang Gao
    • Wanhong He
    • Minjie Xu
    • Qihan Wu
    • Xiaohua Ni
    • Handong Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Disease, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200127, P.R. China, NHC Key Laboratory of Reproduction Regulation, Shanghai Institute of Planned Parenthood Research, Fudan University, Shanghai 200032, P.R. China
    Copyright: © Tan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1278-1286
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    Published online on: May 27, 2019
       https://doi.org/10.3892/ol.2019.10400
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Abstract

Despite the clinical requirement for early diagnosis, the early events in lung cancer and their mechanisms are not fully understood. Pituitary tumor transforming gene 1 binding factor (PTTG1IP) is a tumor‑associated gene; however, to the best of our knowledge, its association with lung cancer has not been reported. The present study analyzed PTTG1IP expression in early‑stage non‑small cell lung cancer (NSCLC) samples and investigated its epigenetic regulatory mechanisms. The results revealed that the mRNA level of PTTG1IP in NSCLC tissues was significantly downregulated by 43% compared with that in adjacent tissues. In addition, overexpression of this gene significantly inhibited cell proliferation. According to data from The Cancer Genome Atlas, a significant negative correlation was identified between the PTTG1IP gene methylation level and expression level in lung adenocarcinoma and lung squamous cell carcinoma cases. Reduced representation bisulfite sequencing (RRBS) analysis of six paired early‑stage NSCLC tissue samples indicated that the CpG island shore of the PTTG1IP promoter is hypermethylated in lung cancer tissues, which was further validated in 12 paired early‑stage NSCLC samples via bisulfite amplicon sequencing. Following treatment with 5‑aza‑2'‑deoxycytidine to reduce DNA methylation in the promoter region, the PTTG1IP mRNA level increased, indicating that the PTTG1IP promoter DNA methylation level negatively regulates PTTG1IP transcription. In conclusion, in early‑stage NSCLC, the PTTG1IP gene is regulated by DNA methylation in its promoter region, which may participate in the development and progression of lung cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Tan X, Zhang S, Gao H, He W, Xu M, Wu Q, Ni X and Jiang H: Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer. Oncol Lett 18: 1278-1286, 2019.
APA
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q. ... Jiang, H. (2019). Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer. Oncology Letters, 18, 1278-1286. https://doi.org/10.3892/ol.2019.10400
MLA
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q., Ni, X., Jiang, H."Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer". Oncology Letters 18.2 (2019): 1278-1286.
Chicago
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q., Ni, X., Jiang, H."Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer". Oncology Letters 18, no. 2 (2019): 1278-1286. https://doi.org/10.3892/ol.2019.10400
Copy and paste a formatted citation
x
Spandidos Publications style
Tan X, Zhang S, Gao H, He W, Xu M, Wu Q, Ni X and Jiang H: Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer. Oncol Lett 18: 1278-1286, 2019.
APA
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q. ... Jiang, H. (2019). Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer. Oncology Letters, 18, 1278-1286. https://doi.org/10.3892/ol.2019.10400
MLA
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q., Ni, X., Jiang, H."Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer". Oncology Letters 18.2 (2019): 1278-1286.
Chicago
Tan, X., Zhang, S., Gao, H., He, W., Xu, M., Wu, Q., Ni, X., Jiang, H."Hypermethylation of the PTTG1IP promoter leads to low expression in early‑stage non‑small cell lung cancer". Oncology Letters 18, no. 2 (2019): 1278-1286. https://doi.org/10.3892/ol.2019.10400
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