LncRNA HAND2‑AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1

Liu,Pengcheng; Du,Ruili; Yu,Xin

doi:10.3892/ol.2019.10402

LncRNA HAND2‑AS1 overexpression inhibits cancer cell proliferation in melanoma by downregulating ROCK1

Authors:
- Pengcheng Liu
- Ruili Du
- Xin Yu
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Affiliations: Department of Hand and Pediatric Surgery, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Clinical Laboratory, The Second Hospital of Changchun City, Changchun, Jilin 130022, P.R. China
Published online on: May 27, 2019 https://doi.org/10.3892/ol.2019.10402
Pages: 1005-1010
Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding (lnc)RNA heart and neural crest derivatives expressed 2‑antisense RNA 1 (HAND2‑AS1), an lncRNA antisense transcript adjacent to the heart and neural crest derivatives expressed 2 protein (HAND2), inhibits the development of several types of cancer; however, its role in melanoma is currently unknown. In the present study, it was revealed that lncRNA HAND2‑AS1 was downregulated, whereas Rho‑associated protein kinase 1 (ROCK1) mRNA was upregulated in tumor tissues when compared with the healthy tissues of patients with melanoma. Expression levels of lncRNA HAND2‑AS1 and ROCK1 were inversely correlated in tumor tissues, but not in healthy tissues. LncRNA HAND2‑AS1 expression levels were affected by tumor thickness, but not by tumor metastasis. LncRNA HAND2‑AS1 overexpression led to inhibited melanoma cancer cell proliferation and an increased expression level of ROCK1. ROCK1 overexpression caused no evident effects on lncRNA HAND2‑AS1 expression, but promoted cancer cell proliferation and decreased the effects of lncRNA HAND2‑AS1 overexpression on cancer cell proliferation. Thus, it is possible that lncRNA HAND2‑AS1 overexpression leads to inhibited cancer cell proliferation in melanoma cells through the downregulation of ROCK1.

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