Low expression of miR‑125a‑5p is associated with poor prognosis in patients with gastric cancer

Li,Guan; Ao,Sheng; Hou,Jianing; Lyu,Guoqing

doi:10.3892/ol.2019.10423

Low expression of miR‑125a‑5p is associated with poor prognosis in patients with gastric cancer

Authors:
- Guan Li
- Sheng Ao
- Jianing Hou
- Guoqing Lyu
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Affiliations: Department of Gastrointestinal Surgery, Peking University Shenzhen Hospital, Shenzhen, Guangdong 518036, P.R. China
Published online on: May 31, 2019 https://doi.org/10.3892/ol.2019.10423
Pages: 1483-1490
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

microRNAs (miRs) serve critical roles in tumor progression. Low expression of miR‑125a in gastric carcinoma (GC) may promote tumor development. In the present study, low expression of miR‑125a was confirmed in cancer tissues using The Cancer Genome Atlas database. Additionally, the expression and clinical significance of miR‑125a‑5p was investigated using reverse transcription‑quantitative PCR in 150 cases of GC. The results of the present study demonstrated that the level of miR‑125a‑5p expression was decreased in GC biopsies compared with that in matched adjacent normal tissues. Low expression of miR‑125a‑5p was associated with increased tumor diameter, high Ki67 expression and poor overall survival of patients with GC. Multivariate survival analysis demonstrated that low miR‑125a‑5p expression may be used as an independent prognostic factor for patients with GC. However, no effects on the cell viability in a Cell Counting kit‑8 assay, and cell migration and invasion in Transwell assays were detected in response to treatment using miR‑125a‑5p mimics or inhibitors in vitro. Therefore, the results of the present study provide evidence that low expression of miR‑125a‑5p may be associated with a poor prognosis, suggesting its value as a tumor biomarker for patients with GC.

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