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Article

Significant association of EED promoter hypomethylation with colorectal cancer

  • Authors:
    • Xiuru Ying
    • Ranran Pan
    • Jie Zhong
    • Boyi Wu
    • Yuting Jiang
    • Jieer Ying
    • Cong Zhou
    • Jie Dai
    • Shuangying Zhao
    • Yinan Shen
    • Wei Zhang
    • Shiwei Duan
  • View Affiliations / Copyright

    Affiliations: Medical Genetics Center, School of Medicine, Ningbo University, Ningbo, Zhejiang 315211, P.R. China, Department of Medical Oncology, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China, Department of Preventive Medicine and The Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Feinberg School of Medicine, Chicago, IL 60611, USA
  • Pages: 1564-1570
    |
    Published online on: June 4, 2019
       https://doi.org/10.3892/ol.2019.10432
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Abstract

Colorectal cancer (CRC) is one of the most common and serious types of malignancy worldwide. The embryonic ectoderm development (EED) gene is important to maintain transcriptional repressive states of genes over successive cell generations. The present study aimed to investigate the association between EED methylation and CRC. A total of 111 CRC tissue samples, 111 paired para‑tumor tissues and 20 colorectal normal tissues were obtained for EED methylation assay, which was performed using a quantitative methylation‑specific polymerase chain reaction. The percentage of methylated reference was calculated to represent the DNA methylation level. A dual‑luciferase reporter gene assay was used to detect the gene promoter activity of a EED fragment. The current results revealed a significant difference in the EED methylation levels among tumor, para‑tumor and normal colorectal tissues (tumor vs. para‑tumor vs. normal, 5.03±4.61 vs. 8.65±11.50 vs. 40.12±45.31; F=45.014; P<0.0001). The dual‑luciferase reporter gene assay demonstrated that the transcriptional activity of recombinant pGL3‑EED plasmid was significantly higher compared with that of the pGL3‑Basic control vector (fold‑change, 3.15; P=0.014), which suggests the EED fragment can promote gene expression. In conclusion, the present study demonstrated that EED hypomethylation may be an important factor associated with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Ying X, Pan R, Zhong J, Wu B, Jiang Y, Ying J, Zhou C, Dai J, Zhao S, Shen Y, Shen Y, et al: Significant association of EED promoter hypomethylation with colorectal cancer. Oncol Lett 18: 1564-1570, 2019.
APA
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J. ... Duan, S. (2019). Significant association of EED promoter hypomethylation with colorectal cancer. Oncology Letters, 18, 1564-1570. https://doi.org/10.3892/ol.2019.10432
MLA
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J., Zhou, C., Dai, J., Zhao, S., Shen, Y., Zhang, W., Duan, S."Significant association of EED promoter hypomethylation with colorectal cancer". Oncology Letters 18.2 (2019): 1564-1570.
Chicago
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J., Zhou, C., Dai, J., Zhao, S., Shen, Y., Zhang, W., Duan, S."Significant association of EED promoter hypomethylation with colorectal cancer". Oncology Letters 18, no. 2 (2019): 1564-1570. https://doi.org/10.3892/ol.2019.10432
Copy and paste a formatted citation
x
Spandidos Publications style
Ying X, Pan R, Zhong J, Wu B, Jiang Y, Ying J, Zhou C, Dai J, Zhao S, Shen Y, Shen Y, et al: Significant association of EED promoter hypomethylation with colorectal cancer. Oncol Lett 18: 1564-1570, 2019.
APA
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J. ... Duan, S. (2019). Significant association of EED promoter hypomethylation with colorectal cancer. Oncology Letters, 18, 1564-1570. https://doi.org/10.3892/ol.2019.10432
MLA
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J., Zhou, C., Dai, J., Zhao, S., Shen, Y., Zhang, W., Duan, S."Significant association of EED promoter hypomethylation with colorectal cancer". Oncology Letters 18.2 (2019): 1564-1570.
Chicago
Ying, X., Pan, R., Zhong, J., Wu, B., Jiang, Y., Ying, J., Zhou, C., Dai, J., Zhao, S., Shen, Y., Zhang, W., Duan, S."Significant association of EED promoter hypomethylation with colorectal cancer". Oncology Letters 18, no. 2 (2019): 1564-1570. https://doi.org/10.3892/ol.2019.10432
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