Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma

Chen,Shunguang; Xu,Xiaoming; Lu,Shengjun; Hu,Biao

doi:10.3892/ol.2019.10445

Long non‑coding RNA HAND2‑AS1 targets glucose metabolism and inhibits cancer cell proliferation in osteosarcoma

Authors:
- Shunguang Chen
- Xiaoming Xu
- Shengjun Lu
- Biao Hu
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Affiliations: Department of Orthopedics, Jingzhou Central Hospital, Jingzhou, Hubei 434020, P.R. China
Published online on: June 7, 2019 https://doi.org/10.3892/ol.2019.10445
Pages: 1323-1329
Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Long non‑coding RNA heart and neural crest derivatives expressed 2‑antisense RNA 1 (lncRNA HAND2‑AS1) is a known tumor suppressor gene in endometrioid endometrial carcinoma; however, its function in osteosarcoma is currently unknown. In the present study, HAND2‑AS1 expression in the tumor tissues and adjacent healthy tissues of patients with osteosarcoma, and in the serum of patients and heathy controls was detected by reverse transcription‑quantitative polymerase chain reaction. lncRNA HAND2‑AS1 small interfering RNA was transfected into osteosarcoma cells, and cell proliferation, glucose transporter 1 (GLUT1) expression and glucose uptake were detected using the Cell Counting Kit‑8, western blotting and glucose uptake assays, respectively. The results revealed that the expression levels of HAND2‑AS1 were reduced in cancer tissues compared with those in healthy tissues. Levels of HAND2‑AS1 were also reduced in the serum of patients with osteosarcoma compared with those of the control subjects. A significant association was observed between serum levels of HAND2‑AS1 and tumor size, but not tumor metastasis. HAND2‑AS1‑knockdown promoted osteosarcoma cell proliferation, increased glucose uptake and upregulated GLUT1 expression. It was therefore concluded that lncRNA HAND2‑AS1 may inhibit the proliferation of osteosarcoma cells by targeting glucose metabolism.

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