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Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway

  • Authors:
    • Xiuchun Wang
    • Xuemei Wang
  • View Affiliations / Copyright

    Affiliations: Department of Vascular Intervention, Affiliated Hospital of Weifang Medical University, Weifang, Shandong 261031, P.R. China, Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1745-1754
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    Published online on: June 18, 2019
       https://doi.org/10.3892/ol.2019.10488
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Abstract

The aim of this study was to investigate how long non‑coding (lnc)RNA colon cancer‑associated transcript 2 (CCAT2) regulates the proliferation, invasion and metastasis of esophageal cancer cells via the Wnt signaling pathway. The expression of lncRNA CCAT2 was quantified by reverse transcription‑quantitative PCR in four esophageal cancer cell lines (Eca‑109, EC9706, KYSE150 and TE‑1) and normal human esophageal epithelial cells (HEECs). The effect of silencing CCAT2 (si‑CCAT2) and inhibiting Wnt signaling (using the inhibitor FH535) on the proliferation, migration and invasion of Eca‑109 cells was measured by MTT, wound‑healing and Transwell invasion assays. Flow cytometry was used to evaluate apoptosis in si‑CCAT2 Eca‑109 cells. The expression of β‑catenin and proliferating cell nuclear antigen (PCNA) proteins was detected by immunohistochemistry. The pro‑apoptotic protein Bax, cyclin D1 and Wnt target proteins, including c‑Myc and adenomatous polyposis coli (APC), were detected by western blotting. LncRNA CCAT2 was highly expressed in the four esophageal cancer cell lines compared with the HEEC cells. The expression of CCAT2 was significantly decreased in si‑CCAT2 Eca‑109 cells. Treatment with si‑CCAT2 and FH535 alone or in combination significantly inhibited the proliferation, migration and invasion of Eca‑109 cells. The treatments also promoted apoptosis, upregulated the expression of Bax and APC proteins, and downregulated β‑catenin, PCNA, cyclin D1 and c‑Myc proteins. In summary, lncRNA CCAT2 is upregulated in esophageal cancer cells and the knockdown of lncRNA CCAT2 inhibits their proliferation, migration and invasion via the Wnt signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X and Wang X: Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway. Oncol Lett 18: 1745-1754, 2019.
APA
Wang, X., & Wang, X. (2019). Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway. Oncology Letters, 18, 1745-1754. https://doi.org/10.3892/ol.2019.10488
MLA
Wang, X., Wang, X."Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway". Oncology Letters 18.2 (2019): 1745-1754.
Chicago
Wang, X., Wang, X."Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway". Oncology Letters 18, no. 2 (2019): 1745-1754. https://doi.org/10.3892/ol.2019.10488
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X and Wang X: Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway. Oncol Lett 18: 1745-1754, 2019.
APA
Wang, X., & Wang, X. (2019). Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway. Oncology Letters, 18, 1745-1754. https://doi.org/10.3892/ol.2019.10488
MLA
Wang, X., Wang, X."Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway". Oncology Letters 18.2 (2019): 1745-1754.
Chicago
Wang, X., Wang, X."Long non‑coding RNA colon cancer‑associated transcript 2 may promote esophageal cancer growth and metastasis by regulating the Wnt signaling pathway". Oncology Letters 18, no. 2 (2019): 1745-1754. https://doi.org/10.3892/ol.2019.10488
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