Open Access

miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495 are downregulated in colorectal cancer cells and have putative roles in the mTOR pathway

  • Authors:
    • Naif Alqurashi
    • Saeed M. Hashimi
    • Faisal Alowaidi
    • Saso Ivanovski
    • Amro Farag
    • Ming Q. Wei
  • View Affiliations

  • Published online on: June 21, 2019     https://doi.org/10.3892/ol.2019.10508
  • Pages: 1657-1668
  • Copyright: © Alqurashi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRNAs) are small non‑coding RNAs that regulate gene expression by suppressing the target mRNA and inhibiting translation in order to regulate multiple biological processes. miRNAs play important roles as oncogenes or tumor suppressors in the development of various types of human cancer. The regulation of mammalian target of rapamycin (mTOR) by miRNAs has been studied in several types of cancer, including colorectal cancer (CRC). However, to the best of our knowledge, only limited information regarding the function of miRNAs in human CRC is available. In the present study, the expression of 22 miRNAs in CRC cell lines were investigated in regard to key genes in the mTOR pathway. Initially, it was revealed that mTOR, regulatory‑associated protein of mTOR complex I and rapamycin‑intensive companion of mTOR were overexpressed in CRC cell lines when compared with a normal colorectal cell line. Subsequently, putative miRNA‑mRNA associations were identified via multiple miRNA target prediction programs. The expression levels for the candidate miRNAs were validated using quantitative real‑time polymerase chain reaction. Expression analysis revealed that, among 20 miRNAs, five miRNAs (miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495) exhibited significant downregulation in association with the mTOR signaling pathway. Taken together, the results from the present study suggest that several miRNAs that are associated with CRC, with possible roles in mTOR signaling, may have potential therapeutic or diagnostic benefits in CRC treatment.
View Figures
View References

Related Articles

Journal Cover

August-2019
Volume 18 Issue 2

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Alqurashi N, Hashimi SM, Alowaidi F, Ivanovski S, Farag A and Wei MQ: miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495 are downregulated in colorectal cancer cells and have putative roles in the mTOR pathway. Oncol Lett 18: 1657-1668, 2019
APA
Alqurashi, N., Hashimi, S.M., Alowaidi, F., Ivanovski, S., Farag, A., & Wei, M.Q. (2019). miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495 are downregulated in colorectal cancer cells and have putative roles in the mTOR pathway. Oncology Letters, 18, 1657-1668. https://doi.org/10.3892/ol.2019.10508
MLA
Alqurashi, N., Hashimi, S. M., Alowaidi, F., Ivanovski, S., Farag, A., Wei, M. Q."miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495 are downregulated in colorectal cancer cells and have putative roles in the mTOR pathway". Oncology Letters 18.2 (2019): 1657-1668.
Chicago
Alqurashi, N., Hashimi, S. M., Alowaidi, F., Ivanovski, S., Farag, A., Wei, M. Q."miR‑496, miR‑1185, miR‑654, miR‑3183 and miR‑495 are downregulated in colorectal cancer cells and have putative roles in the mTOR pathway". Oncology Letters 18, no. 2 (2019): 1657-1668. https://doi.org/10.3892/ol.2019.10508