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Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2

  • Authors:
    • Yu Ma
    • Mingyue Hu
    • Lingna Zhou
    • Sunkai Ling
    • Yuan Li
    • Bo Kong
    • Peilin Huang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, School of Medicine, Southeast University, Nanjing, Jiangsu 210009, P.R. China, Department of Surgery, School of Medicine, Technical University Munich, D‑80333 Munich, Germany
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2212-2219
    |
    Published online on: July 2, 2019
       https://doi.org/10.3892/ol.2019.10551
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Abstract

Hox transcript antisense RNA (HOTAIR) is a long non‑coding RNA (lncRNA) that serves a key role in the pathogenesis of various types of cancer, including pancreatic adenocarcinoma. However, the diagnostic and prognostic values of HOTAIR in pancreatic adenocarcinoma, as well as its involvement in cancer cell energy metabolism, remain unclear. In the present study, tumor tissues and adjacent healthy tissues were collected from patients with pancreatic adenocarcinoma, and blood samples were collected from patients and healthy controls. Expression levels of HOTAIR and hexokinase‑2 (HK2) were detected by reverse transcription‑quantitative polymerase chain reaction. All patients were followed up for 5 years, and the diagnostic and prognostic values of serum HOTAIR levels were investigated by receiver operating characteristic curve and survival analyses, respectively. Pancreatic adenocarcinoma cell lines overexpressing HOTAIR and HK2 were established, and the effects on cell proliferation, lactate production, glucose uptake and ATP production were detected by Cell Counting Kit‑8, lactate, glucose uptake and ATP assays, respectively. The protein expression was detected by western blot analysis. The results revealed that HOTAIR and HK2 expression levels were increased in tumor tissues compared with adjacent healthy tissues. The serum levels of HOTAIR and HK2 were higher in patients with pancreatic cancer compared with healthy controls. The serum levels of these two factors may be used to accurately predict pancreatic adenocarcinoma and its prognosis. HOTAIR and HK2 overexpression led to the promotion of tumor cell proliferation. HOTAIR overexpression increased lactate production, glucose uptake and ATP production. Furthermore, it promoted HK2 expression, however HK2 overexpression displayed no significant effects on HOTAIR expression levels. Therefore, it was concluded that the lncRNA HOTAIR may promote cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating HK2.
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1 

Stewart BW and Wild CP: World cancer report 2014. Health. 2017.

2 

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ and He J: Cancer statistics in China, 2015. CA Cancer J Clin. 66:115–132. 2016. View Article : Google Scholar : PubMed/NCBI

3 

Chen W, Zheng R, Zuo T, Zeng H, Zhang S and He J: National cancer incidence and mortality in China, 2012. Chin J Cancer Res. 28:1–11. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Ryan DP, Hong TS and Bardeesy N: Pancreatic adenocarcinoma. N Engl J Med. 371:1039–1049. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Sharma C, Eltawil KM, Renfrew PD, Walsh MJ and Molinari M: Advances in diagnosis, treatment and palliation of pancreatic carcinoma: 1990–2010. World J Gastroenterol. 17:867–897. 2011. View Article : Google Scholar : PubMed/NCBI

6 

Vincent A, Herman J, Schulick R, Hruban RH and Goggins M: Pancreatic cancer. Lancet. 378:607–620. 2011. View Article : Google Scholar : PubMed/NCBI

7 

Mattick JS and Makunin IV: Non-coding RNA. Hum Mol Genet 15 (Spec No 1). R17–R29. 2006. View Article : Google Scholar

8 

Cui Z, Ren S, Lu J, Wang F, Xu W, Sun Y, Wei M, Chen J, Gao X, Xu C, et al: The prostate cancer-up-regulated long noncoding RNA PlncRNA-1 modulates apoptosis and proliferation through reciprocal regulation of androgen receptor. Urol Oncol. 31:1117–1123. 2013. View Article : Google Scholar : PubMed/NCBI

9 

Gupta RA, Shah N, Wang KC, Kim J, Horlings HM, Wong DJ, Tsai MC, Hung T, Argani P, Rinn JL, et al: Long non-coding RNA HOTAIR reprograms chromatin state to promote cancer metastasis. Nature. 464:1071–1076. 2010. View Article : Google Scholar : PubMed/NCBI

10 

Cai H, Yao J, An Y, Chen X, Chen W, Wu D, Luo B, Yang Y, Jiang Y, Sun D and He X: LncRNA HOTAIR acts as competing endogenous RNA to control the expression of Notch3 via sponging miR-613 in pancreatic cancer. Oncotarget. 8:32905–32917. 2017.PubMed/NCBI

11 

Kamarajah SK, Burns WR, Frankel TL, Cho CS and Nathan H: Validation of the American Joint Commission on Cancer (AJCC) 8th edition staging system for patients with pancreatic adenocarcinoma: A Surveillance, Epidemiology and End Results (SEER) Analysis. Ann Surg Oncol. 24:2023–2030. 2017. View Article : Google Scholar : PubMed/NCBI

12 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

13 

Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Pang EJ, Yang R, Fu XB and Liu YF: Overexpression of long non-coding RNA MALAT1 is correlated with clinical progression and unfavorable prognosis in pancreatic cancer. Tumour Biol. 36:2403–2407. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Jiang Y, Li Z, Zheng S, Chen H, Zhao X, Gao W, Bi Z, You K, Wang Y, Li W, et al: The long non-coding RNA HOTAIR affects the radiosensitivity of pancreatic ductal adenocarcinoma by regulating the expression of Wnt inhibitory factor 1. Tumour Biol. 37:3957–3967. 2016. View Article : Google Scholar : PubMed/NCBI

16 

Nie H, Zhang Y, Xing R, Li M and Mou Y: Epigenetic silence of HOTAIR contributes to the metastasis of pancreatic cancer via targeting miR-138. Oncol Res. Dec 21–2017.(Epub ahead of print). View Article : Google Scholar

17 

Fadaka A, Ajiboye B, Ojo O, Adewale O and Olayide I: Biology of glucose metabolization in cancer cells. J Oncol Sci. 3:45–51. 2017.

18 

Mathupala SP, Ko YH and Pedersen PL: Hexokinase-2 bound to mitochondria: Cancer's stygian link to the ‘Warburg Effect’ and a pivotal target for effective therapy. Semin Cancer Biol. 19:17–24. 2009. View Article : Google Scholar : PubMed/NCBI

19 

Katagiri M, Karasawa H, Takagi K, Nakayama S, Yabuuchi S, Fujishima F, Naitoh T, Watanabe M, Suzuki T, Unno M and Sasano H: Hexokinase 2 in colorectal cancer: A potent prognostic factor associated with glycolysis, proliferation and migration. Histol Histopathol. 32:351–360. 2017.PubMed/NCBI

20 

Ono H, Motoi N, Nagano H, Miyauchi E, Ushijima M, Matsuura M, Okumura S, Nishio M, Hirose T, Inase N and Ishikawa Y: Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in small-cell lung cancer. Cancer Med. 3:632–642. 2014. View Article : Google Scholar : PubMed/NCBI

21 

Cao R, Saracini C, Ginsbach JW, Kieber-Emmons MT, Siegler MA, Solomon EI, Fukuzumi S and Karlin KD: Peroxo and superoxo moieties bound to copper ion: Electron-transfer equilibrium with a small reorganization energy. J Am Chem Soc. 138:7055–7066. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Ma Y, Hu M, Zhou L, Ling S, Li Y, Kong B and Huang P: Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2. Oncol Lett 18: 2212-2219, 2019.
APA
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., & Huang, P. (2019). Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2. Oncology Letters, 18, 2212-2219. https://doi.org/10.3892/ol.2019.10551
MLA
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., Huang, P."Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2". Oncology Letters 18.3 (2019): 2212-2219.
Chicago
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., Huang, P."Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2". Oncology Letters 18, no. 3 (2019): 2212-2219. https://doi.org/10.3892/ol.2019.10551
Copy and paste a formatted citation
x
Spandidos Publications style
Ma Y, Hu M, Zhou L, Ling S, Li Y, Kong B and Huang P: Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2. Oncol Lett 18: 2212-2219, 2019.
APA
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., & Huang, P. (2019). Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2. Oncology Letters, 18, 2212-2219. https://doi.org/10.3892/ol.2019.10551
MLA
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., Huang, P."Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2". Oncology Letters 18.3 (2019): 2212-2219.
Chicago
Ma, Y., Hu, M., Zhou, L., Ling, S., Li, Y., Kong, B., Huang, P."Long non‑coding RNA HOTAIR promotes cancer cell energy metabolism in pancreatic adenocarcinoma by upregulating hexokinase‑2". Oncology Letters 18, no. 3 (2019): 2212-2219. https://doi.org/10.3892/ol.2019.10551
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