Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells

Ding,Ku‑Ke; Yang,Fen; Jiang,Hui‑Qing; Yuan,Zeng‑Qiang; Yin,Ling‑Ling; Dong,Ling‑Yue; Cui,Wei; Gou,Qiao; Liu,Xiao‑Dan; Wu,Yu‑Mei; Jiang,Xiao‑Yan; Zhang,Xin; Zhou,Ping‑Kun; Yang,Chuan‑Jie

doi:10.3892/ol.2019.10590

Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells

Authors:
- Ku‑Ke Ding
- Fen Yang
- Hui‑Qing Jiang
- Zeng‑Qiang Yuan
- Ling‑Ling Yin
- Ling‑Yue Dong
- Wei Cui
- Qiao Gou
- Xiao‑Dan Liu
- Yu‑Mei Wu
- Xiao‑Yan Jiang
- Xin Zhang
- Ping‑Kun Zhou
- Chuan‑Jie Yang
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Affiliations: National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, P.R. China, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, P.R. China, Biomedical Engineering School and Foundation Medical School, Capital Medical University, Beijing 100069, P.R. China, Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China, Department of Gynecological Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, P.R. China, Department of Gynecology, Liaoning Cancer Hospital and Cancer Hospital of China Medical University, Shenyang, Liaoning 110042, P.R. China
Published online on: July 9, 2019 https://doi.org/10.3892/ol.2019.10590
Pages: 2704-2711
Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The effects of the immediate early response 5 (IER5) gene on the sensitivity of HeLa cells to radiation remain unclear. In the present study, stably transfected HeLa cells resulting in the knockdown or overexpression of IER5 were investigated. In addition, xenografts of normal, IER5‑silenced and ‑overexpressed HeLa cells were injected into nude mice and examined. The results demonstrated that the radiosensitivity of the IER5‑overexpressed HeLa cells was significantly increased compared with that of the normal and IER5‑silenced cells. The upregulation of IER5 effectively decreased cell proliferation and IER5 silencing promoted cell proliferation compared with that in the normal HeLa cells. Following irradiation of the cells with IER5 knockdown, cell cycle was arrested at the G2/M phase and an increase in the proportion of S phase cells was observed. By contrast, the overexpression of IER5 led to an increase in the proportion of G1 phase cells. Furthermore, the upregulation of IER5 inhibited tumor growth in vivo. The present findings demonstrate that the IER5 gene affects the radiosensitivity of HeLa cells and serves an important role in cell proliferation, suggesting that this gene may be a potential radiotherapeutic target in cervical cancer.

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