Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
September-2019 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
September-2019 Volume 18 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells

  • Authors:
    • Ku‑Ke Ding
    • Fen Yang
    • Hui‑Qing Jiang
    • Zeng‑Qiang Yuan
    • Ling‑Ling Yin
    • Ling‑Yue Dong
    • Wei Cui
    • Qiao Gou
    • Xiao‑Dan Liu
    • Yu‑Mei Wu
    • Xiao‑Yan Jiang
    • Xin Zhang
    • Ping‑Kun Zhou
    • Chuan‑Jie Yang
  • View Affiliations / Copyright

    Affiliations: National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, P.R. China, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Institute of Biophysics, The Chinese Academy of Sciences, Beijing 100101, P.R. China, Biomedical Engineering School and Foundation Medical School, Capital Medical University, Beijing 100069, P.R. China, Department of Radiation Toxicology and Oncology, Beijing Institute of Radiation Medicine, Beijing 100850, P.R. China, Department of Gynecological Oncology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100006, P.R. China, Department of Gynecology, Liaoning Cancer Hospital and Cancer Hospital of China Medical University, Shenyang, Liaoning 110042, P.R. China
    Copyright: © Ding et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2704-2711
    |
    Published online on: July 9, 2019
       https://doi.org/10.3892/ol.2019.10590
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The effects of the immediate early response 5 (IER5) gene on the sensitivity of HeLa cells to radiation remain unclear. In the present study, stably transfected HeLa cells resulting in the knockdown or overexpression of IER5 were investigated. In addition, xenografts of normal, IER5‑silenced and ‑overexpressed HeLa cells were injected into nude mice and examined. The results demonstrated that the radiosensitivity of the IER5‑overexpressed HeLa cells was significantly increased compared with that of the normal and IER5‑silenced cells. The upregulation of IER5 effectively decreased cell proliferation and IER5 silencing promoted cell proliferation compared with that in the normal HeLa cells. Following irradiation of the cells with IER5 knockdown, cell cycle was arrested at the G2/M phase and an increase in the proportion of S phase cells was observed. By contrast, the overexpression of IER5 led to an increase in the proportion of G1 phase cells. Furthermore, the upregulation of IER5 inhibited tumor growth in vivo. The present findings demonstrate that the IER5 gene affects the radiosensitivity of HeLa cells and serves an important role in cell proliferation, suggesting that this gene may be a potential radiotherapeutic target in cervical cancer.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Yu C, Zhu W, Ji Y, Guo J, Pan P, Han J and Zhou X: A comparative study of intensity-modulated radiotherapy and standard radiation field with concurrent chemotherapy for local advanced cervical cancer. Eur J Gynaecol Oncol. 36:278–282. 2015.PubMed/NCBI

2 

Fu ZC, Wang FM and Cai JM: Gene expression changes in residual advanced cervical cancer after radiotherapy: Indicators of poor prognosis and radioresistance? Med Sci Monit. 21:1276–1287. 2015. View Article : Google Scholar : PubMed/NCBI

3 

Chu TY, Yang JT, Huang TH and Liu HW: Crosstalk with cancer-associated fibroblasts increases the growth and radiation survival of cervical cancer cells. Radiat Res. 181:540–547. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Xiang L, Xie G, Liu C, Zhou J, Chen J, Yu S, Li J, Pang X, Shi H and Liang H: Knock-down of glutaminase 2 expression decreases glutathione, NADH, and sensitizes cervical cancer to ionizing radiation. Biochim Biophys Acta. 1833:2996–3005. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Yuan W, Xiaoyun H, Haifeng Q, Jing L, Weixu H, Ruofan D, Jinjin Y and Zongji S: MicroRNA-218 enhances the radiosensitivity of human cervical cancer via promoting radiation induced apoptosis. Int J Med Sci. 11:691–696. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Ding KK, Shang ZF, Hao C, Xu QZ, Shen JJ, Yang CJ, Xie YH, Qiao C, Wang Y, Xu LL and Zhou PK: Induced expression of the IER5 gene by gamma-ray irradiation and its involvement in cell cycle checkpoint control and survival. Radiat Environ Biophys. 48:205–213. 2009. View Article : Google Scholar : PubMed/NCBI

7 

Kis E, Szatmári T, Keszei M, Farkas R, Esik O, Lumniczky K, Falus A and Sáfrány G: Microarray analysis of radiation response genes in primary human fibroblasts. Int J Radiat Oncol Biol Phys. 66:1506–1514. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

9 

Huang XQ, Chen X, Xie XX, Zhou Q, Li K, Li S, Shen LF and Su J: Co-expression of CD147 and GLUT-1 indicates radiation resistance and poor prognosis in cervical squamous cell carcinoma. Int J Clin Exp Pathol. 7:1651–1666. 2014.PubMed/NCBI

10 

Gaffney DK, Jhingran A, Portelance L, Viswanathan A, Schefter T, Weidhaas J and Small W Jr: Radiation therapy oncology group gynecologic oncology working group: Comprehensive results. Int J Gynecol Cancer. 24:956–962. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Zheng L, Tang W, Wei F, Wang H, Liu J, Lu Y, Cheng Y, Bai X, Yu X and Zhao W: Radiation-inducible protein RbAp48 contributes to radiosensitivity of cervical cancer cells. Gynecol Oncol. 130:601–608. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Moreno-Acosta P, Gamboa O, Sanchez de Gomez M, Cendales R, Diaz GD, Romero A, Balart Serra J, Conrado Z, Levy A, Chargari C and Magné N: IGF1R gene expression as a predictive marker of response to ionizing radiation for patients with locally advanced HPV16-positive cervical cancer. Anticancer Res. 32:4319–4325. 2012.PubMed/NCBI

13 

Halle C, Andersen E, Lando M, Aarnes EK, Hasvold G, Holden M, Syljuåsen RG, Sundfør K, Kristensen GB, Holm R, et al: Hypoxia-induced gene expression in chemoradioresistant cervical cancer revealed by dynamic contrast-enhanced MRI. Cancer Res. 72:5285–5295. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Baiocchi G, Begnami MD, Fukazawa EM, Oliveira RA, Faloppa CC, Kumagai LY, Badiglian-Filho L, Pellizzon AC, Maia MA, Jacinto AA, et al: Prognostic value of nuclear factor kappa B expression in patients with advanced cervical cancer undergoing radiation therapy followed by hysterectomy. J Clin Pathol. 65:614–618. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Paul S, Barker CA, Turner HC, McLane A, Wolden SL and Amundson SA: Prediction of in vivo radiation dose status in radiotherapy patients using ex vivo and in vivo gene expression signatures. Radiat Res. 175:257–265. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Kim WY, Lee JW, Park YA, Choi JJ, Sung CO, Song SY, Choi CH, Kim TJ, Huh SJ, Kim BG and Bae DS: RAR-beta expression is associated with early volumetric changes to radiation therapy in cervical cancer. Gynecol Obstet Invest. 71:11–18. 2011. View Article : Google Scholar : PubMed/NCBI

17 

Kabacik S, Mackay A, Tamber N, Manning G, Finnon P, Paillier F, Ashworth A, Bouffler S and Badie C: Gene expression following ionising radiation: Identification of biomarkers for dose estimation and prediction of individual response. Int J Radiat Biol. 87:115–129. 2011. View Article : Google Scholar : PubMed/NCBI

18 

Filiano AN, Fathallah-Shaykh HM, Fiveash J, Gage J, Cantor A, Kharbanda S and Johnson MR: Gene expression analysis in radiotherapy patients and C57BL/6 mice as a measure of exposure to ionizing radiation. Radiat Res. 176:49–61. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Williams M, Lyu MS, Yang YL, Lin EP, Dunbrack R, Birren B, Cunningham J and Hunter K: Ier5, a novel member of the slow-kinetics immediate-early genes. Genomics. 55:327–334. 1999. View Article : Google Scholar : PubMed/NCBI

20 

Göttgens B, Barton LM, Chapman MA, Sinclair AM, Knudsen B, Grafham D, Gilbert JG, Rogers J, Bentley DR and Green AR: Transcriptional regulation of the stem cell leukemia gene (SCL)-comparative analysis of five vertebrate SCL loci. Genome Res. 12:749–759. 2002. View Article : Google Scholar : PubMed/NCBI

21 

Cirelli C and Tononi G: Gene expression in the brain across the sleep-waking cycle. Brain Res. 885:303–321. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Zeng F, Hon CC, Sit WH, Chow KY, Hui RK, Law IK, Ng VW, Yang XT, Leung FC and Wan JM: Molecular characterization of Coriolus versicolor PSP-induced apoptosis in human promyelotic leukemic HL-60 cells using cDNA microarray. Int J Oncol. 27:513–523. 2005.PubMed/NCBI

23 

Nakamura S, Nagata Y, Tan L, Takemura T, Shibata K, Fujie M, Fujisawa S, Tanaka Y, Toda M, Makita R, et al: Transcriptional repression of Cdc25B by IER5 inhibits the proliferation of leukemic progenitor cells through NF-YB and p300 in acute myeloid leukemia. PLoS One. 6:e280112011. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Ding KK, Yang F, Jiang HQ, Yuan ZQ, Yin LL, Dong LY, Cui W, Gou Q, Liu XD, Wu YM, Wu YM, et al: Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells. Oncol Lett 18: 2704-2711, 2019.
APA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L. ... Yang, C. (2019). Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells. Oncology Letters, 18, 2704-2711. https://doi.org/10.3892/ol.2019.10590
MLA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18.3 (2019): 2704-2711.
Chicago
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18, no. 3 (2019): 2704-2711. https://doi.org/10.3892/ol.2019.10590
Copy and paste a formatted citation
x
Spandidos Publications style
Ding KK, Yang F, Jiang HQ, Yuan ZQ, Yin LL, Dong LY, Cui W, Gou Q, Liu XD, Wu YM, Wu YM, et al: Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells. Oncol Lett 18: 2704-2711, 2019.
APA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L. ... Yang, C. (2019). Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells. Oncology Letters, 18, 2704-2711. https://doi.org/10.3892/ol.2019.10590
MLA
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18.3 (2019): 2704-2711.
Chicago
Ding, K., Yang, F., Jiang, H., Yuan, Z., Yin, L., Dong, L., Cui, W., Gou, Q., Liu, X., Wu, Y., Jiang, X., Zhang, X., Zhou, P., Yang, C."Overexpression of the immediate early response 5 gene increases the radiosensitivity of HeLa cells". Oncology Letters 18, no. 3 (2019): 2704-2711. https://doi.org/10.3892/ol.2019.10590
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team