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Article Open Access

MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway

  • Authors:
    • Jianying Cui
    • Guodong Pan
    • Qingjuan He
    • Lizhi Yin
    • Rui Guo
    • Hongmei Bi
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, Anqiu People's Hospital, Weifang, Shandong 262100, P.R. China, Department of Thoracic Surgery, Traditional Chinese Medical Hospital of Huangdao District, Qingdao, Shandong 266500, P.R. China, Department of Respiratory Medicine, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Health Management Center, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Outpatient Department, The People's Hospital of Zhangqiu Area, Jinan, Shandong 250200, P.R. China, Department of Respiratory Medicine, The Third People's Hospital of Qingdao, Qingdao, Shandong 266041, P.R. China
    Copyright: © Cui et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2931-2938
    |
    Published online on: July 15, 2019
       https://doi.org/10.3892/ol.2019.10619
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Abstract

The specific function of microRNA-545 (miR-545) has been reported to regulate the development of human cancers. However, the effect of miR-545 is still unclear in non‑small cell lung cancer (NSCLC). Hence, this study explored the molecular mechanism of miR-545 in NSCLC. The expression levels of miR-545 and ZEB2 were measured through reverse transcription‑quantitative polymerase chain reaction (RT-qPCR) assay. The protein expression was detected by western blotting. Dual luciferase assay was applied to evaluate the relationship between miR-545 and zinc finger E-box‑binding homeobox 2 (ZEB2). MTT and Transwell assays were used to investigate the function of miR-545 in NSCLC. The expression of miR-545 was decreased in NSCLC tissues. The overexpression of miR-545 suppressed the migration, invasion and proliferation of NSCLC cells. Furthermore, ZEB2 was a direct target gene of miR-545. The knockout of ZEB2 suppressed the development of NSCLC. miR-545 inhibited the progression of NSCLC through targeting ZEB2. Moreover, miR-545 repressed the development of NSCLC via blocking EMT and Wnt/β-catenin pathway. In conclusion, miR-545 inhibited the progression of NSCLC through targeting ZEB2 and blocking EMT and Wnt/β-catenin pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Cui J, Pan G, He Q, Yin L, Guo R and Bi H: MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway. Oncol Lett 18: 2931-2938, 2019.
APA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., & Bi, H. (2019). MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway. Oncology Letters, 18, 2931-2938. https://doi.org/10.3892/ol.2019.10619
MLA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18.3 (2019): 2931-2938.
Chicago
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18, no. 3 (2019): 2931-2938. https://doi.org/10.3892/ol.2019.10619
Copy and paste a formatted citation
x
Spandidos Publications style
Cui J, Pan G, He Q, Yin L, Guo R and Bi H: MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway. Oncol Lett 18: 2931-2938, 2019.
APA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., & Bi, H. (2019). MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway. Oncology Letters, 18, 2931-2938. https://doi.org/10.3892/ol.2019.10619
MLA
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18.3 (2019): 2931-2938.
Chicago
Cui, J., Pan, G., He, Q., Yin, L., Guo, R., Bi, H."MicroRNA-545 targets ZEB2 to inhibit the development of non‑small cell lung cancer by inactivating Wnt/β-catenin pathway". Oncology Letters 18, no. 3 (2019): 2931-2938. https://doi.org/10.3892/ol.2019.10619
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