MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1

Corrigendum in: /10.3892/ol.2023.14032

  • Authors:
    • Hong‑Lin Li
    • Jun‑Jie Sun
    • Hui Ma
    • Shen‑Jia Liu
    • Na Li
    • Su‑Jie Guo
    • Yang Shi
    • Yan‑Ying Xu
    • Zhi‑Ying Qi
    • Yu‑Quan Wang
    • Fang Wang
    • Rui‑Meng Guo
    • Dong Liu
    • Feng‑Xia Xue
  • View Affiliations

  • Published online on: July 31, 2019     https://doi.org/10.3892/ol.2019.10694
  • Pages: 3792-3802
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Abstract

The present study focused on exploring the inhibitory mechanism of microRNA (miR)‑23a in endometrial cancer. Reverse transcription quantitative polymerase chain reaction (RT‑qPCR) was used to investigate miR‑23a expression in endometrial tissues and endometrial cancer cells. A colony formation assay using crystal violet staining was performed to compare cell proliferation, while wound‑healing and Transwell assays were performed to compare cell migration and invasion. Subsequently, bioinformatics and a luciferase reporter gene assay were used to investigate the effect of miR‑23a on sine oculis homeobox homolog 1 (SIX1) expression, and the biological function of SIX1 was analyzed. Additionally, a nude mouse tumorigenicity assay was performed to test the inhibitory effect of miR‑23a and Taxol® therapy in endometrial cancer. Finally, immunohistochemistry and RT‑qPCR were used to explore the association between miR‑23a and SIX1 expression in endometrial cancer tissues. miR‑23a was underexpressed in endometrial cancer tissues compared with in para‑carcinoma tissues, and the overexpression of miR‑23a inhibited proliferation and invasion of endometrial cancer cells. Furthermore, SIX1 was demonstrated to be a downstream target of miR‑23a, and miR‑23a reduced SIX1 expression. Additionally, SIX1 inversely promoted cell proliferation, migration and invasion. In addition, the effects of reduced cell proliferation and increased cell invasion following miR‑23a overexpression could be reversed by adding SIX1 to in vitro culture. Furthermore, the inhibitory effect of miR‑23a and Taxol therapy, which reduced SIX1 expression in endometrial cancer, was demonstrated in vivo. Finally, a negative association between miR‑23a and SIX1 expression was demonstrated in endometrial cancer tissues. The results of the present study revealed that miR‑23a may inhibit endometrial cancer development by targeting SIX1.
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October-2019
Volume 18 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Li HL, Sun JJ, Ma H, Liu SJ, Li N, Guo SJ, Shi Y, Xu YY, Qi ZY, Wang YQ, Wang YQ, et al: MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1 Corrigendum in /10.3892/ol.2023.14032. Oncol Lett 18: 3792-3802, 2019
APA
Li, H., Sun, J., Ma, H., Liu, S., Li, N., Guo, S. ... Xue, F. (2019). MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1 Corrigendum in /10.3892/ol.2023.14032. Oncology Letters, 18, 3792-3802. https://doi.org/10.3892/ol.2019.10694
MLA
Li, H., Sun, J., Ma, H., Liu, S., Li, N., Guo, S., Shi, Y., Xu, Y., Qi, Z., Wang, Y., Wang, F., Guo, R., Liu, D., Xue, F."MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1 Corrigendum in /10.3892/ol.2023.14032". Oncology Letters 18.4 (2019): 3792-3802.
Chicago
Li, H., Sun, J., Ma, H., Liu, S., Li, N., Guo, S., Shi, Y., Xu, Y., Qi, Z., Wang, Y., Wang, F., Guo, R., Liu, D., Xue, F."MicroRNA‑23a inhibits endometrial cancer cell development by targeting SIX1 Corrigendum in /10.3892/ol.2023.14032". Oncology Letters 18, no. 4 (2019): 3792-3802. https://doi.org/10.3892/ol.2019.10694