Long non‑coding RNA GATA6‑AS inhibits gastric cancer cell proliferation by downregulating microRNA‑25‑3p

Li,Dingyun; Wang,Ting; Lai,Jiajun; Zhang,Tao; Zhu,Xiaofeng; Zeng,Deqiang; Hu,Zhiwei

doi:10.3892/ol.2019.10803

Long non‑coding RNA GATA6‑AS inhibits gastric cancer cell proliferation by downregulating microRNA‑25‑3p

Authors:
- Dingyun Li
- Ting Wang
- Jiajun Lai
- Tao Zhang
- Xiaofeng Zhu
- Deqiang Zeng
- Zhiwei Hu
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Affiliations: Department of Gastrointestinal Surgery, Yue Bei People's Hospital, Shaoguan, Guangdong 512026, P.R. China, Department of Physical Diagnostics, Yue Bei People's Hospital, Shaoguan, Guangdong 512026, P.R. China
Published online on: September 4, 2019 https://doi.org/10.3892/ol.2019.10803
Pages: 4639-4644
Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The abnormal growth of endothelial cells is involved in several types of diseases, including gastric cancer. The long non‑coding RNA GATA6‑AS is a key regulator of endothelial cell growth and may therefore also play an important role in gastric cancer. In the present study it was found that GATA6‑AS was downregulated in tumor tissues compared with adjacent normal tissues. Moreover, plasma levels of GATA6‑AS were linearly associated with GATA6‑AS expression levels in tumor tissues and not in normal tissues. MicroRNA (miR)‑25‑3p was upregulated in tumor tissues compared with adjacent normal tissues and was inversely associated with GATA6‑AS in tumor tissues only. The overexpression of miR‑25‑3p in gastric cancer cells resulted in no significant changes in the expression levels of GATA6‑AS, whereas overexpression of GATA6‑AS led to significantly downregulated miR‑25‑3p levels. Furthermore, overexpression of GATA6‑AS inhibited cancer cell proliferation, with no effect on migration and invasion. The overexpression of miR‑25‑3p resulted in increased proliferation of cancer cells and attenuated the effects of GATA6‑AS overexpression. Thus, it is postulated that GATA6‑AS inhibits proliferation of gastric cancer cells by downregulating miR‑25‑3p.

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