Open Access

miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway

  • Authors:
    • Xiangqin Tang
    • Yanjun Ding
    • Xiaoqing Wang
    • Xiuzhen Wang
    • Lin Zhao
    • Hongmei Bi
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  • Published online on: September 4, 2019     https://doi.org/10.3892/ol.2019.10805
  • Pages: 4621-4628
  • Copyright: © Tang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Non-small cell lung cancer (NSCLC) is the most frequent cancer worldwide with a poor 5-year survival. miR-650 acts as an oncogene and regulates tumor progress in various cancers. Molecular mechanisms of miR-650 in NSCLC cell proliferation and invasion was studied. The mRNA levels of miR-650 and special genes were calculated using RT-qPCR. MTT and transwell assays were applied to measure the proliferative and invasive ability. Kaplan-Meier method was used to assess the survival of NSCLC patients. miR-650 was upregulated in NSCLC and upregulation of miR-650 was associated with a poor overall survival of NSCLC, while the results of ING4 demonstrated the opposite results. miR-650 promoted proliferation and invasion through Wnt-1/β‑catenin pathway by targeting inhibitor of growth 4 (ING4) in A549 cells. ING4 was a direct target gene of miR-650 and the expression of ING4 was mediated by exogenous altering the expression of miR-650. Remarkably, alterations of ING4 expression eliminated the functions of miR-650 on the proliferation and metastasis of NSCLC. miR-650 enhanced A549 cell proliferation and invasion through Wnt-1/β-catenin pathway by directly targeting the 3'-UTR of ING4 mRNA. The newly identified miR-650/ING4 axis provides a novel insight into the pathogenesis of NSCLC.
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November-2019
Volume 18 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Tang X, Ding Y, Wang X, Wang X, Zhao L and Bi H: miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway . Oncol Lett 18: 4621-4628, 2019
APA
Tang, X., Ding, Y., Wang, X., Wang, X., Zhao, L., & Bi, H. (2019). miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway . Oncology Letters, 18, 4621-4628. https://doi.org/10.3892/ol.2019.10805
MLA
Tang, X., Ding, Y., Wang, X., Wang, X., Zhao, L., Bi, H."miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway ". Oncology Letters 18.5 (2019): 4621-4628.
Chicago
Tang, X., Ding, Y., Wang, X., Wang, X., Zhao, L., Bi, H."miR-650 promotes non-small cell lung cancer cell proliferation and invasion by targeting ING4 through Wnt-1/β-catenin pathway ". Oncology Letters 18, no. 5 (2019): 4621-4628. https://doi.org/10.3892/ol.2019.10805