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Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma

  • Authors:
    • Chuangui Zeng
    • Hai Kuang
    • Wan Fan
    • Xueru Chen
    • Tao Yu
    • Qinchao Tang
    • Zhuoqian Zhou
    • Feixin Liang
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, College of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
    Copyright: © Zeng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4771-4777
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    Published online on: September 9, 2019
       https://doi.org/10.3892/ol.2019.10828
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Abstract

The aim of the present study was to investigate the effects of the transcription factor forkhead box P3 (FOXP3) in neutrophils on the progression of oral squamous cell carcinoma (OSCC). Cancer tissue samples and paracarcinoma tissues were collected from 23 patients with OSCC for the current study. In addition, SCC‑9, a human tongue carcinoma cell line, was co‑cultured with primary human neutrophils and treated with recombinant interleukin 8 (IL‑8). The effect of FOXP3 on the proliferation of SCC‑9 cells was analyzed using a Cell Counting Kit 8 assay. FOXP3 expression in neutrophils was analyzed by quantitative PCR following IL‑8 treatment. FOXP3 protein expression in neutrophils and the amount of IL‑8 protein in the OSCC tumor microenvironment were determined by immunofluorescence analysis. The present study demonstrated that IL‑8 downregulated FOXP3 mRNA expression in neutrophils. Neutrophils and peptide P60, a specific inhibitor of FOXP3, increased proliferation of SCC‑9 cells. In patients with OSCC, FOXP3 protein expression in neutrophils of the stage IV group was significantly lower compared with that of the stage II and stage III groups, while IL‑8 protein expression was higher in cancer tissues compared with that in paracarcinoma tissues. In summary, IL‑8 in the tumor microenvironment may recruit neutrophils, and downregulation of FOXP3 in neutrophils by IL‑8 may promote the progression of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Zeng C, Kuang H, Fan W, Chen X, Yu T, Tang Q, Zhou Z and Liang F: Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma. Oncol Lett 18: 4771-4777, 2019.
APA
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q. ... Liang, F. (2019). Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma. Oncology Letters, 18, 4771-4777. https://doi.org/10.3892/ol.2019.10828
MLA
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q., Zhou, Z., Liang, F."Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma". Oncology Letters 18.5 (2019): 4771-4777.
Chicago
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q., Zhou, Z., Liang, F."Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma". Oncology Letters 18, no. 5 (2019): 4771-4777. https://doi.org/10.3892/ol.2019.10828
Copy and paste a formatted citation
x
Spandidos Publications style
Zeng C, Kuang H, Fan W, Chen X, Yu T, Tang Q, Zhou Z and Liang F: Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma. Oncol Lett 18: 4771-4777, 2019.
APA
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q. ... Liang, F. (2019). Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma. Oncology Letters, 18, 4771-4777. https://doi.org/10.3892/ol.2019.10828
MLA
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q., Zhou, Z., Liang, F."Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma". Oncology Letters 18.5 (2019): 4771-4777.
Chicago
Zeng, C., Kuang, H., Fan, W., Chen, X., Yu, T., Tang, Q., Zhou, Z., Liang, F."Downregulation of FOXP3 in neutrophils by IL‑8 promotes the progression of oral squamous cell carcinoma". Oncology Letters 18, no. 5 (2019): 4771-4777. https://doi.org/10.3892/ol.2019.10828
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