Open Access

MicroRNA‑152 suppresses cisplatin resistance in A549 cells

Corrigendum in: /10.3892/ol.2020.11422

  • Authors:
    • Wenfei Zhao
    • Hongyun Li
    • Shanshan Yang
    • Di Guo
    • Jing Chen
    • Shaoyi Miao
    • Yi Xin
    • Miaomiao Liang
  • View Affiliations

  • Published online on: September 10, 2019     https://doi.org/10.3892/ol.2019.10834
  • Pages: 4613-4620
  • Copyright: © Zhao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to investigate the association between microRNA‑152 and cisplatin resistance in non‑small cell lung cancer. A549 and cisplatin‑resistant A549 cells (A549/cis) were maintained in vitro. Reverse transcription‑quantitative PCR (RT‑qPCR) was performed to analyze differences in microRNA‑152 levels between A549 and A549/cis cells, and changes in Bcl‑2 and NF‑κB expression levels were analyzed via RT‑qPCR and western blot analyses. MicroRNA‑152 was overexpressed in A549/cis cells via transfection of a microRNA‑152 mimic. Upon treating transfected or untransfected A549/cis cells with 2 µg/l cisplatin for 24 h, a Cell Counting Kit‑8 assay, morphological analysis and flow cytometry analysis were performed to evaluate the effect of microRNA‑152 on the inhibition of cell proliferation and induction of apoptosis. Furthermore, changes in Bcl‑2 and NF‑κB expression levels in microRNA‑152‑overexpressing A549/cis cells were also analyzed. MicroRNA‑152 was significantly downregulated and Bcl‑2 and NF‑κB were significantly upregulated in A549/cis cells (P<0.05). MicroRNA‑152 upregulation enhanced the inhibitory effect of cisplatin on A549/cis cells. These results suggest that microRNA‑152 downregulates Bcl‑2 and NF‑κB. MicroRNA‑152 downregulation may induce cisplatin resistance in non‑small cell lung cancer cells, whereas microRNA‑152 upregulation may improve cisplatin sensitivity among A549/cis cells via downregulation of Bcl‑2 and NF‑κB.
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November-2019
Volume 18 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhao W, Li H, Yang S, Guo D, Chen J, Miao S, Xin Y and Liang M: MicroRNA‑152 suppresses cisplatin resistance in A549 cells Corrigendum in /10.3892/ol.2020.11422. Oncol Lett 18: 4613-4620, 2019
APA
Zhao, W., Li, H., Yang, S., Guo, D., Chen, J., Miao, S. ... Liang, M. (2019). MicroRNA‑152 suppresses cisplatin resistance in A549 cells Corrigendum in /10.3892/ol.2020.11422. Oncology Letters, 18, 4613-4620. https://doi.org/10.3892/ol.2019.10834
MLA
Zhao, W., Li, H., Yang, S., Guo, D., Chen, J., Miao, S., Xin, Y., Liang, M."MicroRNA‑152 suppresses cisplatin resistance in A549 cells Corrigendum in /10.3892/ol.2020.11422". Oncology Letters 18.5 (2019): 4613-4620.
Chicago
Zhao, W., Li, H., Yang, S., Guo, D., Chen, J., Miao, S., Xin, Y., Liang, M."MicroRNA‑152 suppresses cisplatin resistance in A549 cells Corrigendum in /10.3892/ol.2020.11422". Oncology Letters 18, no. 5 (2019): 4613-4620. https://doi.org/10.3892/ol.2019.10834