Open Access

Everolimus inhibits the proliferation and migration of epidermal growth factor receptor‑resistant lung cancer cells A549 via regulating the microRNA‑4328/phosphatase and tensin homolog signaling pathway

  • Authors:
    • Xudong Xiang
    • Li Zhuang
    • Huicheng Chen
    • Xiumei Yang
    • Heng Li
    • Gaofeng Li
    • Jing Yu
  • View Affiliations

  • Published online on: September 19, 2019     https://doi.org/10.3892/ol.2019.10887
  • Pages: 5269-5276
  • Copyright: © Xiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Lung cancer is the most common cancer type worldwide, and investigating novel therapeutics methods for the treatment of chemoresistant lung cancer are of notable clinical significance. Reverse transcription‑quantitative polymerase chain reaction and western blotting assays were performed to analyze the expression levels of phosphatase and tensin homolog (PTEN) and microRNA‑4328 (miR‑4328), and Cell Counting Kit‑8 (CCK‑8) and Transwell migration assays were conducted to evaluate the proliferation and migration of A549 cells, respectively. Everolimus was observed to upregulate the expression of PTEN and inhibit the proliferation and migration of A549 cells in a dose‑dependent manner. The knockdown of PTEN abolished the effects of everolimus on the proliferation and migration of A549 cells, and everolimus was demonstrated to upregulate PTEN, and inhibit the proliferation and migration of A549 cells via downregulating miR‑4328. Collectively, the results of the present study indicate that everolimus inhibited the proliferation and migration of EGFR‑resistant A549 lung cancer cells via regulating the miR‑4328/PTEN signaling pathway.
View Figures
View References

Related Articles

Journal Cover

November-2019
Volume 18 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Xiang X, Zhuang L, Chen H, Yang X, Li H, Li G and Yu J: Everolimus inhibits the proliferation and migration of epidermal growth factor receptor‑resistant lung cancer cells A549 via regulating the microRNA‑4328/phosphatase and tensin homolog signaling pathway. Oncol Lett 18: 5269-5276, 2019
APA
Xiang, X., Zhuang, L., Chen, H., Yang, X., Li, H., Li, G., & Yu, J. (2019). Everolimus inhibits the proliferation and migration of epidermal growth factor receptor‑resistant lung cancer cells A549 via regulating the microRNA‑4328/phosphatase and tensin homolog signaling pathway. Oncology Letters, 18, 5269-5276. https://doi.org/10.3892/ol.2019.10887
MLA
Xiang, X., Zhuang, L., Chen, H., Yang, X., Li, H., Li, G., Yu, J."Everolimus inhibits the proliferation and migration of epidermal growth factor receptor‑resistant lung cancer cells A549 via regulating the microRNA‑4328/phosphatase and tensin homolog signaling pathway". Oncology Letters 18.5 (2019): 5269-5276.
Chicago
Xiang, X., Zhuang, L., Chen, H., Yang, X., Li, H., Li, G., Yu, J."Everolimus inhibits the proliferation and migration of epidermal growth factor receptor‑resistant lung cancer cells A549 via regulating the microRNA‑4328/phosphatase and tensin homolog signaling pathway". Oncology Letters 18, no. 5 (2019): 5269-5276. https://doi.org/10.3892/ol.2019.10887