Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
November-2019 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2019 Volume 18 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT

  • Authors:
    • Natasa Matic
    • Marcus Ressner
    • Emilia Wiechec
    • Karin Roberg
  • View Affiliations / Copyright

    Affiliations: Division of Cell Biology, Department of Clinical and Experimental Medicine, Faculty of Health Sciences, Linköping University, Linköping, Östergötland SE‑58185, Sweden, Center for Medical Image Science and Visualization, Linköping University, Linköping, Östergötland SE‑58185, Sweden
    Copyright: © Matic et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 5155-5162
    |
    Published online on: September 24, 2019
       https://doi.org/10.3892/ol.2019.10916
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The standard treatment for head and neck squamous cell carcinoma (HNSCC) is radiotherapy, often in combination with chemotherapy or surgery. However, a novel monoclonal antibody, cetuximab (Erbitux®), has also been approved for patient therapy. The aim of present study was to develop an in vitro method for the measurement of 18F‑fluoro‑2deoxy‑D‑glucose (FDG) to determine if cellular 18F‑FDG uptake is associated with response to radiotherapy or cetuximab treatment. In the current study, HNSCC cell lines were treated with radiation or with cetuximab. Next, the uptake of 18F‑FDG was measured using a gamma spectrometer (GS). Thereafter, uptake after radiation was measured first with GS and then compared with positron emission tomography (PET)/computed tomography (CT) imaging. Furthermore, the mRNA expression of glucose transporter 1 (GLUT1) was measured following cetuximab treatment via reverse transcription‑quantitative PCR. A study protocol was developed to measure the cellular uptake of 18F‑FDG via gamma‑ray spectrometry and comparable results were obtained with those of clinical PET/CT. The results revealed a decrease in 18F‑FDG after radiation and cetuximab treatment. The uptake of 18F‑FDG following cetuximab treatment was significantly lower in the cetuximab‑sensitive cell line UT‑SCC‑14 compared with the cetuximab‑resistant cell lines UT‑SCC‑2 and UT‑SCC‑45. Furthermore, after treatment with cetuximab for 24 and 48 h, a significant increase in GLUT1 expression was detected in the sensitive cell line compared with the two resistant cell lines. In conclusion, a novel yet reliable method for the measurement of intracellular 18F‑FDG via GS has been developed, and our results indicate that 18F‑FDG uptake is associated with radiation and cetuximab response in HNSCC.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Ferlay J, Soerjomataram I, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 136:E359–E386. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Seiwert TY, Salama JK and Vokes EE: The chemoradiation paradigm in head and neck cancer. Nat Clin Pract Oncol. 4:156–171. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Dassonville O, Formento JL, Francoual M, Ramaioli A, Santini J, Schneider M, Demard F and Milano G: Expression of epidermal growth factor receptor and survival in upper aerodigestive tract cancer. J Clin Oncol. 11:1873–1878. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Bonner JA, Harari PM, Giralt J, Azarnia N, Shin DM, Cohen RB, Jones CU, Sur R, Raben D, Jassem J, et al: Radiotherapy plus cetuximab for squamous-cell carcinoma of the head and neck. N Engl J Med. 354:567–578. 2006. View Article : Google Scholar : PubMed/NCBI

5 

Wu W and Zhao S: Metabolic changes in cancer: Beyond the Warburg effect. Acta Biochim Biophys Sin (Shanghai). 45:18–26. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Yamamoto M, Inohara H and Nakagawa T: Targeting metabolic pathways for head and neck cancers therapeutics. Cancer Metastasis Rev. 36:503–514. 2017. View Article : Google Scholar : PubMed/NCBI

7 

Beloueche-Babari M, Box C, Arunan V, Parkes HG, Valenti M, De Haven Brandon A, Jackson LE, Eccles SA and Leach MO: Acquired resistance to EGFR tyrosine kinase inhibitors alters the metabolism of human head and neck squamous carcinoma cells and xenograft tumours. Br J Cancer. 112:1206–1214. 2015. View Article : Google Scholar : PubMed/NCBI

8 

Kircher MF, Hricak H and Larson SM: Molecular imaging for personalized cancer care. Mol Oncol. 6:182–195. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Baschnagel AM, Wobb JL, Dilworth JT, Williams L, Eskandari M, Wu D, Pruetz BL and Wilson GD: The association of (18)F-FDG PET and glucose metabolism biomarkers GLUT1 and HK2 in p16 positive and negative head and neck squamous cell carcinomas. Radiother Oncol. 117:118–124. 2015. View Article : Google Scholar : PubMed/NCBI

10 

Carvalho KC, Cunha IW, Rocha RM, Ayala FR, Cajaíba MM, Begnami MD, Vilela RS, Paiva GR, Andrade RG and Soares FA: GLUT1 expression in malignant tumors and its use as an immunodiagnostic marker. Clinics (Sao Paulo). 66:965–972. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Kunkel M, Reichert TE, Benz P, Lehr HA, Jeong JH, Wieand S, Bartenstein P, Wagner W and Whiteside TL: Overexpression of Glut-1 and increased glucose metabolism in tumors are associated with a poor prognosis in patients with oral squamous cell carcinoma. Cancer. 97:1015–1024. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Wang YD, Li SJ and Liao JX: Inhibition of glucose transporter 1 (GLUT1) chemosensitized head and neck cancer cells to cisplatin. Technol Cancer Res Treat. 12:525–535. 2013. View Article : Google Scholar : PubMed/NCBI

13 

Jedlinski A, Ansell A, Johansson AC and Roberg K: EGFR status and EGFR ligand expression influence the treatment response of head and neck cancer cell lines. J Oral Pathol Med. 42:26–36. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Jerhammar F, Johansson AC, Ceder R, Welander J, Jansson A, Grafström RC, Söderkvist P and Roberg K: YAP1 is a potential biomarker for cetuximab resistance in head and neck cancer. Oral Oncol. 50:832–839. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Roberg K, Ceder R, Farnebo L, Norberg-Spaak L and Grafstrom RC: Multiple genotypic aberrances associate to terminal differentiation-deficiency of an oral squamous cell carcinoma in serum-free culture. Differentiation. 76:868–880. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Leutenegger CM, Mislin CN, Sigrist B, Ehrengruber MU, Hofmann-Lehmann R and Lutz H: Quantitative real-time PCR for the measurement of feline cytokine mRNA. Vet Immunol Immunopathol. 71:291–305. 1999. View Article : Google Scholar : PubMed/NCBI

17 

Jedlinski A, Garvin S, Johansson AC, Edqvist PH, Ponten F and Roberg K: Cetuximab sensitivity of head and neck squamous cell carcinoma xenografts is associated with treatment-induced reduction in EGFR, pEGFR, and pSrc. J Oral Pathol Med. 46:717–724. 2017. View Article : Google Scholar : PubMed/NCBI

18 

Hentschel M, Paulus T, Mix M, Moser E, Nitzsche EU and Brink I: Analysis of blood flow and glucose metabolism in mammary carcinomas and normal breast: A H2(15)O PET and 18F-FDG PET study. Nucl Med Commun. 28:789–797. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Machtay M, Natwa M, Andrel J, Hyslop T, Anne PR, Lavarino J, Intenzo CM and Keane W: Pretreatment FDG-PET standardized uptake value as a prognostic factor for outcome in head and neck cancer. Head Neck. 31:195–201. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Orabi H, Al-Shareaif A and El Galefi M: Gamma-ray measurements of naturally occurring radioactive sample from Alkharje City. J Radioanal Nucl Chem. 269:99–102. 2006. View Article : Google Scholar

21 

Behzadi A, Ung Y, Lowe V and Deschamps C: The role of positron emission tomography in the management of non-small cell lung cancer. Can J Surg. 52:235–242. 2009.PubMed/NCBI

22 

Gould MK, Maclean CC, Kuschner WG, Rydzak CE and Owens DK: Accuracy of positron emission tomography for diagnosis of pulmonary nodules and mass lesions: A meta-analysis. JAMA. 285:914–924. 2001. View Article : Google Scholar : PubMed/NCBI

23 

Zhao K, Yang SY, Zhou SH, Dong MJ, Bao YY and Yao HT: Fluorodeoxyglucose uptake in laryngeal carcinoma is associated with the expression of glucose transporter-1 and hypoxia-inducible-factor-1α and the phosphoinositide 3-kinase/protein kinase B pathway. Oncol Lett. 7:984–990. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Yamada T, Uchida M, Kwang-Lee K, Kitamura N, Yoshimura T, Sasabe E and Yamamoto T: Correlation of metabolism/hypoxia markers and fluorodeoxyglucose uptake in oral squamous cell carcinomas. Oral Surg Oral Med Oral Pathol Oral Radiol. 113:464–471. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Mason NS, Lopresti BJ, Ruszkiewicz J, Dong X, Joyce S, Leef G, Sen M, Wahed AS, Mathis CA, Grandis JR and Thomas SM: Utility of 3′-[(18)F]fluoro-3′-deoxythymidine as a PET tracer to monitor response to gene therapy in a xenograft model of head and neck carcinoma. Am J Nucl Med Mol Imaging. 3:16–31. 2013.PubMed/NCBI

26 

Miyawaki A, Ikeda R, Hijioka H, Ishida T, Ushiyama M, Nozoe E and Nakamura N: SUVmax of FDG-PET correlates with the effects of neoadjuvant chemoradiotherapy for oral squamous cell carcinoma. Oncol Rep. 23:1205–1212. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Shen LF, Zhao X, Zhou SH, Lu ZJ, Zhao K, Fan J and Zhou ML: In vivo evaluation of the effects of simultaneous inhibition of GLUT-1 and HIF-1alpha by antisense oligodeoxynucleotides on the radiosensitivity of laryngeal carcinoma using micro 18F-FDG PET/CT. Oncotarget. 8:34709–34726. 2017.PubMed/NCBI

28 

Starska K, Forma E, Jozwiak P, Bryś M, Lewy-Trenda I, Brzezińska-Błaszczyk E and Krześlak A: Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer-the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis. Tumour Biol. 36:2309–2321. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Wiechec E, Hansson KT, Alexandersson L, Jonsson JI and Roberg K: Hypoxia mediates differential response to anti-EGFR therapy in HNSCC cells. Int J Mol Sci. 18:E9432017. View Article : Google Scholar : PubMed/NCBI

30 

Ben-Haim S and Ell P: 18F-FDG PET and PET/CT in the evaluation of cancer treatment response. J Nucl Med. 50:88–99. 2009. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Matic N, Ressner M, Wiechec E and Roberg K: In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT. Oncol Lett 18: 5155-5162, 2019.
APA
Matic, N., Ressner, M., Wiechec, E., & Roberg, K. (2019). In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT. Oncology Letters, 18, 5155-5162. https://doi.org/10.3892/ol.2019.10916
MLA
Matic, N., Ressner, M., Wiechec, E., Roberg, K."In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT". Oncology Letters 18.5 (2019): 5155-5162.
Chicago
Matic, N., Ressner, M., Wiechec, E., Roberg, K."In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT". Oncology Letters 18, no. 5 (2019): 5155-5162. https://doi.org/10.3892/ol.2019.10916
Copy and paste a formatted citation
x
Spandidos Publications style
Matic N, Ressner M, Wiechec E and Roberg K: In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT. Oncol Lett 18: 5155-5162, 2019.
APA
Matic, N., Ressner, M., Wiechec, E., & Roberg, K. (2019). In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT. Oncology Letters, 18, 5155-5162. https://doi.org/10.3892/ol.2019.10916
MLA
Matic, N., Ressner, M., Wiechec, E., Roberg, K."In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT". Oncology Letters 18.5 (2019): 5155-5162.
Chicago
Matic, N., Ressner, M., Wiechec, E., Roberg, K."In vitro measurement of glucose uptake after radiation and cetuximab treatment in head and neck cancer cell lines using 18F‑FDG, gamma spectrometry and PET/CT". Oncology Letters 18, no. 5 (2019): 5155-5162. https://doi.org/10.3892/ol.2019.10916
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team