Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement

Ishibashi,Rei; Yoshida,Shuntaro; Odawara,Nariaki; Kishikawa,Takahiro; Kondo,Ryo; Nakada,Ayako; Hakuta,Ryunosuke; Takahara,Naminatsu; Tanaka,Eri; Sekiba,Kazuma; Seimiya,Takahiro; Ohnaga,Takashi; Otsuka,Motoyuki; Koike,Kazuhiko

doi:10.3892/ol.2019.11047

Detection of circulating colorectal cancer cells by a custom microfluid system before and after endoscopic metallic stent placement

Authors:
- Rei Ishibashi
- Shuntaro Yoshida
- Nariaki Odawara
- Takahiro Kishikawa
- Ryo Kondo
- Ayako Nakada
- Ryunosuke Hakuta
- Naminatsu Takahara
- Eri Tanaka
- Kazuma Sekiba
- Takahiro Seimiya
- Takashi Ohnaga
- Motoyuki Otsuka
- Kazuhiko Koike
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Affiliations: Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo 113‑8655, Japan, Central Research Institute, Toyama Industrial Technology Center, Toyama 933‑0981, Japan
Published online on: November 4, 2019 https://doi.org/10.3892/ol.2019.11047
Pages: 6397-6404
Copyright: © Ishibashi et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although the detection of circulating tumor cells (CTCs) should be crucial for future personalized medicine, no efficient and flexible methods have been established. The current study established a polymeric custom‑made chip for capturing CTCs with a high efficiency and flexibility. As an example of clinical application, the effects of self‑expandable metallic stent (SEMS) placement on the release of cancer cells into the blood of patients with colorectal cancer and bowel obstruction were analyzed. This was assessed as the placement of SEMS may cause mechanical damage and physical force to malignant tissue, increasing the risk of cancer cell release into the bloodstream. The present study examined the number of CTCs using a custom‑made chip, before, at 24 h after and at 4 days after SEMS placement in patients with colorectal cancer. The results revealed that, among the 13 patients examined, the number of CTCs was increased in three cases at 24 h after SEMS placement. However, this increase was temporary. The number of CTCs also decreased at 4 days after stent placement in most cases. The CTC chip of the current study detected the number of CD133‑positive cancer stem‑like cells, which did not change, even in the patient whose total number of CTCs temporarily increased. The results indicated that this custom‑made microfluid system can efficiently and flexibly detect CTCs, demonstrating its potential for obtaining information during the management of patients with cancer.

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