Oridonin induces apoptosis in HGC‑27 cells by activating the JNK signaling pathway

Ren,Dong‑Lin; Ghoorun,Roshan  Ara; Wu,Xiao‑Hua; Chen,Hong‑Lei; Zhou,Qian; Wu,Xiao‑Bin

doi:10.3892/ol.2019.11104

Oridonin induces apoptosis in HGC‑27 cells by activating the JNK signaling pathway

Authors:
- Dong‑Lin Ren
- Roshan Ara Ghoorun
- Xiao‑Hua Wu
- Hong‑Lei Chen
- Qian Zhou
- Xiao‑Bin Wu
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Affiliations: Department of Colorectal Surgery, The Sixth Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China, Department of Oncology, Longhu People's Hospital, Shantou, Guangdong 515000, P.R. China, Department of Endoscopy, The Eighth Affiliated Hospital of Sun Yat‑sen University, Shenzhen, Guangdong 518033, P.R. China, Department of Gastrointestinal Surgery, The Eighth Affiliated Hospital of Sun Yat‑sen University, Shenzhen, Guangdong 518033, P.R. China
Published online on: November 14, 2019 https://doi.org/10.3892/ol.2019.11104
Pages: 255-260
Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Gastric cancer (GC) is a very common type of cancer. Although current treatment modalities include surgical resection and chemotherapy, many patients are either not eligible for radical resection or have a poor response to chemotherapy. Due to the complex features of the disease, there is a need for complementary therapy. In the present study, the effects of oridonin on cell proliferation, invasion and apoptosis were assessed in the HGC‑27 cell line using the Cell Counting Kit‑8 assay, real‑time cell analysis, and an Annexin V‑FITC/propidium iodide (PI) detection kit, respectively. The effect of oridonin on apoptosis, through the JNK pathway, was also investigated using western blotting. The present study demonstrated that oridonin can suppress cell viability and inhibit cell proliferation by inducing G2/M arrest. Oridonin also induced caspase‑dependent apoptosis in cells by activating the phosphorylated‑JNK/C‑JUN pathway. These results demonstrate the potential of oridonin as a potential therapeutic compound for the treatment of GC.

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